Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M1I95_RS07005 Genome accession   NZ_CP096885
Coordinates   1439936..1440973 (+) Length   345 a.a.
NCBI ID   WP_318549570.1    Uniprot ID   -
Organism   Rossellomorea marisflavi strain B3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1434936..1445973
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1I95_RS06990 (M1I95_06990) - 1436080..1436847 (+) 768 WP_318549567.1 PRC-barrel domain-containing protein -
  M1I95_RS06995 (M1I95_06995) - 1436861..1438258 (+) 1398 WP_318549568.1 VanW family protein -
  M1I95_RS07000 (M1I95_07000) - 1438272..1439927 (+) 1656 WP_318549569.1 GspE/PulE family protein -
  M1I95_RS07005 (M1I95_07005) pilT 1439936..1440973 (+) 1038 WP_318549570.1 type IV pilus twitching motility protein PilT Machinery gene
  M1I95_RS07010 (M1I95_07010) - 1440974..1442185 (+) 1212 WP_318549571.1 type II secretion system F family protein -
  M1I95_RS07015 (M1I95_07015) - 1442293..1442736 (+) 444 WP_318549572.1 type II secretion system protein -
  M1I95_RS07020 (M1I95_07020) - 1442738..1443481 (+) 744 WP_318549573.1 prepilin peptidase -
  M1I95_RS07025 (M1I95_07025) pilM 1443496..1444431 (+) 936 WP_318549574.1 type IV pilus biogenesis protein PilM -
  M1I95_RS07030 (M1I95_07030) - 1444428..1445018 (+) 591 WP_318549575.1 PilN domain-containing protein -
  M1I95_RS07035 (M1I95_07035) - 1445015..1445692 (+) 678 WP_318549576.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37684.33 Da        Isoelectric Point: 7.6729

>NTDB_id=684754 M1I95_RS07005 WP_318549570.1 1439936..1440973(+) (pilT) [Rossellomorea marisflavi strain B3]
MKDKIDALLKSAYELGASDIHLTVGAPPVFRIHGDLKRYGKDALSPETTEGMAKALIAPPMYTSFEDAGELDFSYGIPGV
SRFRINAFRQRSCVSLAIRVIPTSIPTMEDLFLPHALKGIAGRPQGLFLVTGPTGSGKSTTLASIIDHMNRSMRKHIITL
EDPIEYLHRHGSSIIDQREVGFDTGSFSRGLRSALRQDPDIILVGEMRDLETIHTAITAAETGHLVLATLHTSSAPATIE
RIVDVFPPEQQAQIRVQLASVLTGILSQRLFPTVDRKGRRAATELLLNNAAVANLIRSEKVHQIQNVMQTSRASGMHLMS
DSVQGLYEAGIVSKEMALPYIREGA

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=684754 M1I95_RS07005 WP_318549570.1 1439936..1440973(+) (pilT) [Rossellomorea marisflavi strain B3]
ATGAAAGATAAAATTGATGCGCTCCTCAAAAGTGCATATGAACTCGGGGCCTCGGACATCCACCTGACCGTCGGGGCACC
TCCGGTATTCCGCATCCACGGTGACCTGAAACGGTACGGGAAAGATGCCCTCTCCCCGGAAACGACCGAGGGGATGGCCA
AAGCCTTGATTGCCCCGCCCATGTATACGAGTTTTGAAGACGCAGGCGAACTTGATTTTTCCTATGGCATCCCGGGCGTA
TCCCGGTTCAGGATCAATGCTTTCCGGCAGCGCTCCTGTGTATCCCTGGCCATCCGGGTGATCCCGACATCGATTCCGAC
AATGGAGGACCTGTTCCTTCCCCATGCGCTGAAAGGGATTGCCGGAAGGCCCCAGGGCCTCTTCCTCGTGACGGGTCCGA
CGGGCAGTGGGAAATCCACGACCCTTGCGTCCATCATCGATCATATGAACAGGTCGATGAGGAAGCACATCATCACCCTC
GAGGACCCCATCGAGTATCTTCATCGCCACGGCAGTTCCATCATCGATCAGCGGGAGGTCGGATTTGACACGGGCTCTTT
CTCAAGGGGGTTGAGGAGTGCCTTGAGGCAGGATCCAGATATCATCCTCGTAGGGGAAATGAGGGATCTTGAAACGATCC
ATACGGCCATCACGGCTGCTGAGACGGGTCACCTCGTGCTGGCGACCCTTCATACCTCCAGTGCGCCGGCAACCATCGAG
CGCATCGTGGACGTGTTCCCGCCTGAGCAACAGGCCCAGATCCGCGTCCAGCTTGCGTCCGTCCTGACGGGCATCCTTTC
GCAGCGCCTGTTCCCGACCGTGGACCGGAAAGGCCGCCGTGCTGCGACTGAGCTTCTATTGAATAATGCGGCAGTGGCCA
ACCTCATCCGCTCGGAGAAAGTGCATCAGATCCAAAACGTCATGCAGACGTCGCGGGCTTCGGGCATGCATCTTATGAGC
GACAGCGTCCAGGGACTGTACGAGGCGGGGATCGTCTCCAAGGAAATGGCGCTGCCTTATATCCGGGAGGGGGCGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.497

96.812

0.499
  pilT Pseudomonas stutzeri DSM 10701

46.547

96.522

0.449
  pilT Pseudomonas aeruginosa PAK

46.547

96.522

0.449
  pilT Neisseria meningitidis 8013

46.407

96.812

0.449
  pilT Neisseria gonorrhoeae MS11

46.108

96.812

0.446
  pilT Acinetobacter nosocomialis M2

45.946

96.522

0.443
  pilT Acinetobacter baumannii D1279779

45.946

96.522

0.443
  pilT Acinetobacter baumannii strain A118

45.946

96.522

0.443
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.921

95.942

0.441
  pilT Vibrio cholerae strain A1552

45.921

95.942

0.441
  pilT Legionella pneumophila strain Lp02

45.045

96.522

0.435
  pilT Legionella pneumophila strain ERS1305867

45.045

96.522

0.435
  pilT Acinetobacter baylyi ADP1

44.377

95.362

0.423
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.101

0.403
  pilU Vibrio cholerae strain A1552

40.683

93.333

0.38
  pilU Acinetobacter baylyi ADP1

39.385

94.203

0.371