Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GsYM18_RS07325 Genome accession   NZ_AP017912
Coordinates   1593600..1594697 (+) Length   365 a.a.
NCBI ID   WP_119332459.1    Uniprot ID   -
Organism   Geobacter sulfurreducens strain YM18     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1588600..1599697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GsYM18_RS07310 (YM18_1450) - 1588965..1590836 (+) 1872 WP_119332457.1 DUF2723 domain-containing protein -
  GsYM18_RS07315 (YM18_1451) aroE 1590839..1591699 (+) 861 WP_119332458.1 shikimate dehydrogenase -
  GsYM18_RS07320 (YM18_1452) pilB 1591765..1593471 (+) 1707 WP_045668408.1 type IV-A pilus assembly ATPase PilB -
  GsYM18_RS07325 (YM18_1453) pilT 1593600..1594697 (+) 1098 WP_119332459.1 type IV pilus twitching motility protein PilT Machinery gene
  GsYM18_RS07330 (YM18_1454) - 1594734..1595951 (+) 1218 WP_119332460.1 type II secretion system F family protein -
  GsYM18_RS07335 (YM18_1455) - 1595955..1597622 (+) 1668 WP_119334060.1 two-component system sensor histidine kinase NtrB -
  GsYM18_RS07340 (YM18_1456) - 1597641..1599017 (+) 1377 WP_119332461.1 sigma-54-dependent transcriptional regulator -
  GsYM18_RS17365 (YM18_1457) - 1599237..1599458 (+) 222 WP_119332462.1 type IV pilin protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40715.09 Da        Isoelectric Point: 7.9038

>NTDB_id=68469 GsYM18_RS07325 WP_119332459.1 1593600..1594697(+) (pilT) [Geobacter sulfurreducens strain YM18]
MANMHQLLTELVNRGGSDLHITTNSPPQIRVDGQLIPLEMPPLNAVDTKQLCYSILTEQQKHKFEEANELDLSFGIKGLS
RFRGNVFIQRGAVAGVFRVIPYKILTFEELGLPAVVKELAEKPRGLILVTGPTGSGKSTTLAAIIDKINTERHDHIVTIE
DPIEYLHPHKSCVVNQREVGADTKSFKNALKYILRQDPDVVLVGELRDLETIEAALTLAETGHLCLATLHTNSAVQTINR
IVDVFPPYQQPQVRAQLSFVLEGVMSQTLLPNVSGKGRVLALEVMVPNPAIRNLIREDKIHQIYSQMQVGQEKFGMQTMN
QSLFSLLQKRRISLDVAMARSSDPDELKQMLTSAQRPPGQRPQMR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=68469 GsYM18_RS07325 WP_119332459.1 1593600..1594697(+) (pilT) [Geobacter sulfurreducens strain YM18]
ATGGCCAACATGCATCAACTCCTCACGGAACTCGTCAATCGCGGGGGCTCGGACCTTCACATCACCACTAACTCGCCCCC
GCAGATCCGGGTCGACGGCCAGTTGATCCCTCTCGAGATGCCACCGCTCAATGCGGTGGACACCAAGCAGCTCTGCTACA
GCATCCTTACCGAGCAGCAGAAGCACAAATTCGAGGAAGCCAACGAACTCGACCTTTCTTTCGGCATCAAGGGGCTTTCC
CGTTTCCGGGGTAACGTGTTCATTCAGCGGGGCGCAGTTGCCGGCGTGTTCCGGGTGATCCCCTACAAGATTCTCACCTT
TGAAGAGCTGGGTCTGCCGGCGGTGGTGAAAGAACTGGCGGAGAAGCCGCGAGGGCTGATCCTCGTTACCGGTCCCACCG
GCAGCGGCAAGTCGACCACCCTGGCCGCCATCATCGACAAGATCAACACCGAGCGGCACGATCATATCGTAACTATTGAG
GATCCCATCGAATACCTGCATCCTCACAAAAGTTGCGTGGTGAACCAGCGCGAGGTGGGGGCCGATACGAAAAGCTTCAA
GAACGCTCTTAAATACATCCTGCGCCAGGATCCGGACGTGGTCCTCGTGGGGGAGCTCCGGGATCTGGAGACCATCGAGG
CGGCCCTGACCCTTGCCGAGACCGGTCACCTCTGCCTCGCCACGCTCCACACAAACTCGGCGGTGCAGACCATCAACCGG
ATTGTGGACGTATTCCCCCCCTACCAGCAGCCTCAGGTCCGTGCTCAGCTTTCTTTCGTTCTGGAAGGGGTCATGTCCCA
GACCCTGCTCCCGAACGTATCGGGCAAGGGGAGGGTGCTGGCCCTGGAGGTGATGGTGCCCAATCCGGCCATCCGTAACC
TGATCCGTGAAGACAAGATCCACCAGATATATTCCCAGATGCAGGTGGGGCAGGAGAAATTCGGCATGCAGACCATGAAC
CAGTCGCTGTTCAGTCTGTTGCAGAAGAGACGGATTTCGCTTGATGTGGCCATGGCCCGTTCGTCCGATCCAGACGAACT
CAAGCAGATGCTGACCAGCGCCCAGCGGCCACCGGGCCAGCGCCCTCAGATGAGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

55.891

90.685

0.507
  pilT Legionella pneumophila strain Lp02

55.891

90.685

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.705

96.438

0.499
  pilT Acinetobacter baylyi ADP1

51.014

94.521

0.482
  pilT Pseudomonas stutzeri DSM 10701

51.014

94.521

0.482
  pilT Pseudomonas aeruginosa PAK

51.014

94.521

0.482
  pilT Acinetobacter baumannii D1279779

50.435

94.521

0.477
  pilT Acinetobacter baumannii strain A118

50.435

94.521

0.477
  pilT Acinetobacter nosocomialis M2

50.435

94.521

0.477
  pilT Neisseria meningitidis 8013

49.102

91.507

0.449
  pilT Vibrio cholerae strain A1552

49.541

89.589

0.444
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

89.589

0.444
  pilT Neisseria gonorrhoeae MS11

48.048

91.233

0.438
  pilU Vibrio cholerae strain A1552

42.571

95.89

0.408
  pilU Pseudomonas stutzeri DSM 10701

41.69

97.26

0.405
  pilU Acinetobacter baylyi ADP1

39.344

100

0.395

Multiple sequence alignment