Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CPY96_RS09095 Genome accession   NZ_AP017630
Coordinates   2062672..2063724 (-) Length   350 a.a.
NCBI ID   WP_096516436.1    Uniprot ID   A0AAP4A4N9
Organism   Clostridium perfringens strain CBA7123     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2057672..2068724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPY96_RS09070 sigE 2058265..2058972 (-) 708 WP_003449468.1 RNA polymerase sporulation sigma factor SigE -
  CPY96_RS09075 spoIIGA 2058986..2059792 (-) 807 WP_096516432.1 sigma-E processing peptidase SpoIIGA -
  CPY96_RS09080 - 2059818..2060015 (-) 198 WP_157734682.1 hypothetical protein -
  CPY96_RS09085 ftsZ 2060197..2061342 (-) 1146 WP_096516434.1 cell division protein FtsZ -
  CPY96_RS09090 - 2061356..2062594 (-) 1239 WP_096516435.1 cell division FtsA domain-containing protein -
  CPY96_RS09095 pilT 2062672..2063724 (-) 1053 WP_096516436.1 type IV pilus twitching motility protein PilT Machinery gene
  CPY96_RS09100 - 2063905..2064177 (-) 273 Protein_1784 sigma-70 family RNA polymerase sigma factor -
  CPY96_RS09105 - 2064283..2064684 (-) 402 WP_096516437.1 hypothetical protein -
  CPY96_RS09110 - 2064773..2065828 (-) 1056 WP_096516438.1 GH25 family lysozyme -
  CPY96_RS09115 - 2065878..2066315 (-) 438 WP_096516439.1 phage holin family protein -
  CPY96_RS09120 - 2066357..2068048 (-) 1692 WP_096516440.1 phage tail spike protein -
  CPY96_RS09125 - 2068060..2068623 (-) 564 WP_096516441.1 hypothetical protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38992.62 Da        Isoelectric Point: 5.4454

>NTDB_id=68256 CPY96_RS09095 WP_096516436.1 2062672..2063724(-) (pilT) [Clostridium perfringens strain CBA7123]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPDTLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINSNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQTIISQQLVETVDGDRTAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
SNLYREGVITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=68256 CPY96_RS09095 WP_096516436.1 2062672..2063724(-) (pilT) [Clostridium perfringens strain CBA7123]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAAGCGTATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGGGTTATATCTTTAGATATGCCAACTTTAGA
TAGCTTAGGATATCCAGATACTTTAAAGGATATATGCAATCTTAAAAGAGGCCTTGTATTAGTTACAGGTCCTACTGGAA
GTGGTAAAAGTACAACTTTAGCTGCTTTAATAAATGAAATAAATAGTAATAGAGAATCTCATATAATAACAATAGAGGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAAAGAGAGATAGGTAAGGATACTTTAAGTTATGAAAG
AGCTTTAAAAGCAGCTCTTAGAGAAGATCCTGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACGGCGGCAGAGACTGGACATTTAGTATTTTCAACTCTTCACACCATAGGAGCTGCTAAGACTATAGATAGAATA
GTGGATGTTTTTCCACCACATCAACAGGAGCAAATAAAAATACAATTAGCTTCTGTTTTACAAACAATTATATCACAGCA
ATTAGTAGAAACAGTTGATGGAGATAGAACTGCCGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GGGAAGGGAAAACTCATCAAATAGAATCATCCATACAAACAGGAAGTAAATATGGAATGAGAACAATGGATATGGAACTT
TCAAATCTTTACAGAGAAGGTGTAATAACACAAGAAACTGCAATGAACTCTGCTATAGATAGGGAAATTCTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.422

98.857

0.489
  pilT Neisseria meningitidis 8013

49.709

98.286

0.489
  pilT Neisseria gonorrhoeae MS11

49.709

98.286

0.489
  pilT Legionella pneumophila strain ERS1305867

49.123

97.714

0.48
  pilT Legionella pneumophila strain Lp02

49.123

97.714

0.48
  pilT Acinetobacter baumannii strain A118

50

96

0.48
  pilT Acinetobacter nosocomialis M2

50

96

0.48
  pilT Pseudomonas aeruginosa PAK

50

96

0.48
  pilT Acinetobacter baumannii D1279779

50

96

0.48
  pilT Acinetobacter baylyi ADP1

50.301

94.857

0.477
  pilT Pseudomonas stutzeri DSM 10701

49.702

96

0.477
  pilT Vibrio cholerae strain A1552

50.765

93.429

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

93.429

0.474
  pilU Pseudomonas stutzeri DSM 10701

44.277

94.857

0.42
  pilU Vibrio cholerae strain A1552

40.356

96.286

0.389
  pilU Acinetobacter baylyi ADP1

39.823

96.857

0.386

Multiple sequence alignment