Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M1B72_RS13165 Genome accession   NZ_CP096574
Coordinates   3113833..3114936 (-) Length   367 a.a.
NCBI ID   WP_183347392.1    Uniprot ID   A0A6V8MW24
Organism   Geomonas paludis strain RG22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3108833..3119936
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1B72_RS22260 - 3109079..3109306 (-) 228 WP_183347385.1 type IV pilin protein -
  M1B72_RS13150 (M1B72_13150) - 3109522..3110898 (-) 1377 WP_183347387.1 sigma-54 dependent transcriptional regulator -
  M1B72_RS13155 (M1B72_13155) - 3110942..3112564 (-) 1623 WP_183347389.1 nitrogen regulation protein NR(II) -
  M1B72_RS13160 (M1B72_13160) - 3112569..3113792 (-) 1224 WP_183347391.1 type II secretion system F family protein -
  M1B72_RS13165 (M1B72_13165) pilT 3113833..3114936 (-) 1104 WP_183347392.1 type IV pilus twitching motility protein PilT Machinery gene
  M1B72_RS13170 (M1B72_13170) pilB 3114969..3116675 (-) 1707 WP_183347394.1 type IV-A pilus assembly ATPase PilB -
  M1B72_RS13175 (M1B72_13175) - 3116748..3117602 (-) 855 WP_183347396.1 shikimate dehydrogenase -
  M1B72_RS13180 (M1B72_13180) - 3117602..3119458 (-) 1857 WP_183347398.1 DUF2723 domain-containing protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40836.16 Da        Isoelectric Point: 7.5089

>NTDB_id=681831 M1B72_RS13165 WP_183347392.1 3113833..3114936(-) (pilT) [Geomonas paludis strain RG22]
MINFHQMLKELVERGGSDLHITTNTSPQIRIDGRLTPMDVPPLTAIETKQLCYSILTDAQKHRFEEENELDLSFGVKGLS
RFRGNIFVQRGAVAGVFRVIPYKILTFEELGLPPVARALCDKPRGLILVTGPTGSGKSTTLASMIDHININRHDHIVTVE
DPIEYLHPHKGCIVNQREVGADTKSFKHALKYVLRQDPDVVLIGELRDLETIEAALTLAETGHLCFATLHTNSCAQTINR
IIDVFPPYQQTQVRTQLSFVLEGVMSQTLIPRATGPGRALALEVMVPNPAIRNLIREDKVHQIYSQMQVGQEKFGMQTMN
QCLMGLLSRRLITVDDALGRSSEPDELKQMLAPGGSGAAQIRRPPQR

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=681831 M1B72_RS13165 WP_183347392.1 3113833..3114936(-) (pilT) [Geomonas paludis strain RG22]
ATGATCAACTTTCATCAAATGCTCAAGGAACTGGTGGAGAGGGGCGGCTCCGACCTCCACATCACCACCAATACCTCGCC
GCAGATCCGCATCGACGGTAGACTCACCCCGATGGACGTCCCGCCGCTCACCGCCATCGAGACCAAGCAGCTCTGCTACA
GCATCCTGACCGATGCGCAGAAGCACAGGTTCGAAGAGGAAAACGAGCTGGACCTCTCCTTCGGCGTGAAGGGGCTGTCG
CGTTTCAGGGGGAACATCTTCGTGCAGCGCGGCGCGGTCGCCGGCGTCTTCAGGGTGATCCCCTACAAGATCCTCACCTT
CGAGGAACTGGGGCTTCCCCCGGTCGCCAGGGCCCTGTGCGACAAGCCGCGCGGCCTGATCCTGGTCACCGGGCCTACCG
GCTCCGGCAAGTCGACCACGCTCGCCTCCATGATCGACCACATCAACATCAACCGCCACGACCACATCGTGACCGTGGAG
GACCCGATCGAGTACCTGCACCCGCACAAGGGGTGCATCGTGAACCAGCGCGAAGTCGGCGCCGACACCAAGAGCTTCAA
GCACGCCCTGAAGTACGTGCTGCGCCAGGACCCGGACGTGGTCCTGATCGGCGAGTTGCGCGACCTGGAGACCATCGAGG
CGGCGCTGACTCTCGCCGAGACCGGCCACCTCTGCTTCGCCACGCTGCACACCAACTCCTGCGCCCAGACCATCAACAGG
ATCATCGACGTCTTCCCTCCCTACCAGCAGACCCAGGTGAGGACCCAGCTCTCCTTCGTGCTGGAAGGGGTCATGTCCCA
GACCCTGATACCGAGGGCGACCGGGCCGGGACGTGCCCTGGCGCTCGAGGTCATGGTTCCCAACCCGGCGATCAGGAACC
TGATCCGTGAAGACAAGGTGCACCAGATCTACTCGCAGATGCAGGTCGGACAGGAGAAGTTCGGCATGCAGACCATGAAC
CAGTGTCTGATGGGGCTTCTGTCGCGGCGCCTCATCACCGTGGACGATGCCCTGGGCCGCTCCTCCGAACCGGACGAGCT
GAAGCAGATGCTGGCCCCGGGGGGCTCGGGCGCGGCGCAGATCCGGCGCCCGCCGCAAAGATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6V8MW24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.829

98.638

0.501
  pilT Acinetobacter baylyi ADP1

50.867

94.278

0.48
  pilT Acinetobacter nosocomialis M2

50.578

94.278

0.477
  pilT Pseudomonas aeruginosa PAK

50.578

94.278

0.477
  pilT Legionella pneumophila strain Lp02

52.41

90.463

0.474
  pilT Legionella pneumophila strain ERS1305867

52.41

90.463

0.474
  pilT Pseudomonas stutzeri DSM 10701

50.289

94.278

0.474
  pilT Acinetobacter baumannii D1279779

50.289

94.278

0.474
  pilT Acinetobacter baumannii strain A118

50.289

94.278

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.64

90.191

0.439
  pilT Vibrio cholerae strain A1552

48.64

90.191

0.439
  pilT Neisseria meningitidis 8013

46.407

91.008

0.422
  pilT Neisseria gonorrhoeae MS11

45.946

90.736

0.417
  pilU Vibrio cholerae strain A1552

42.033

99.183

0.417
  pilU Pseudomonas stutzeri DSM 10701

41.643

96.185

0.401
  pilU Acinetobacter baylyi ADP1

41.954

94.823

0.398