Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M0644_RS02475 Genome accession   NZ_CP096573
Coordinates   501819..502916 (+) Length   365 a.a.
NCBI ID   WP_149817851.1    Uniprot ID   -
Organism   Thermosynechococcus sp. B1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496819..507916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0644_RS02455 (M0644_02450) - 497532..499007 (-) 1476 WP_286626450.1 right-handed parallel beta-helix repeat-containing protein -
  M0644_RS02460 (M0644_02455) - 499055..499252 (-) 198 WP_024125597.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  M0644_RS02465 (M0644_02460) - 499253..499465 (-) 213 WP_286626452.1 hypothetical protein -
  M0644_RS02470 (M0644_02465) pilB/pilB1 499727..501751 (+) 2025 WP_149817849.1 GspE/PulE family protein Machinery gene
  M0644_RS02475 (M0644_02470) pilT 501819..502916 (+) 1098 WP_149817851.1 type IV pilus twitching motility protein PilT Machinery gene
  M0644_RS02480 (M0644_02475) - 502953..504173 (+) 1221 WP_286626454.1 type II secretion system F family protein -
  M0644_RS02485 (M0644_02480) - 504182..504655 (+) 474 WP_206201315.1 J domain-containing protein -
  M0644_RS02490 (M0644_02485) - 504652..504924 (+) 273 WP_149817855.1 DUF3143 domain-containing protein -
  M0644_RS02495 (M0644_02490) - 504921..505274 (-) 354 WP_149817857.1 hypothetical protein -
  M0644_RS02500 (M0644_02495) - 505569..506714 (-) 1146 WP_286626455.1 cysteine desulfurase family protein -
  M0644_RS02510 (M0644_02505) - 506990..507361 (+) 372 WP_286626456.1 ferredoxin-thioredoxin reductase catalytic domain-containing protein -
  M0644_RS02515 (M0644_02510) - 507358..507813 (-) 456 WP_286626457.1 spore coat U domain-containing protein -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40366.43 Da        Isoelectric Point: 6.7579

>NTDB_id=681811 M0644_RS02475 WP_149817851.1 501819..502916(+) (pilT) [Thermosynechococcus sp. B1]
MELMIEDLMEQVVANGGSDLHISAGLPPYIRISGKLTPTDYEPLTPEQCQRLIFSMLNNTQRKHLEQNWELDCSYGVRGL
ARFRVNVYKDRGTYAACLRALSSKIPTFEQLGLPNIVREMSERPRGLILVTGPTGSGKTTTLAAMIDLINKTRAEHILTI
EDPIEFVYEPIKSLIHQRQVGEDTKSFANALRAALREDPDIILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPEQQQQIRVQLSNSLVAVFSQTLVPKKNPKPGEFGRIMAQEIMVVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYRAGIITYEAAMAKSSRQDELQRLIGTGTPAAAAR

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=681811 M0644_RS02475 WP_149817851.1 501819..502916(+) (pilT) [Thermosynechococcus sp. B1]
ATGGAGCTAATGATTGAAGACCTAATGGAGCAAGTGGTGGCCAATGGTGGCTCCGATTTGCATATCTCCGCTGGCTTACC
CCCCTACATTCGCATTAGCGGCAAATTAACCCCGACGGACTACGAACCACTCACCCCAGAGCAATGCCAGCGCCTGATCT
TCAGCATGCTCAACAATACCCAACGCAAGCACCTAGAGCAAAACTGGGAGTTGGACTGCTCCTATGGTGTACGTGGCCTC
GCCCGGTTCCGCGTCAATGTGTATAAAGACCGGGGAACCTATGCTGCTTGCTTGCGAGCACTCAGTTCCAAAATTCCCAC
GTTTGAGCAGTTGGGATTGCCCAACATTGTCCGCGAAATGAGTGAGCGGCCACGGGGCTTGATTCTGGTGACTGGGCCGA
CAGGATCAGGGAAAACCACAACCTTGGCGGCGATGATTGACTTGATCAACAAAACCCGCGCTGAACATATCCTGACGATT
GAAGACCCCATTGAGTTTGTCTATGAACCCATCAAGAGCCTGATCCACCAACGGCAGGTGGGGGAAGATACCAAGAGCTT
TGCCAATGCTCTGCGGGCAGCGCTGCGGGAAGACCCCGATATTATCCTTGTGGGTGAGATGCGTGACTTGGAAACAATTC
AGCTTGCCATCTCAGCAGCGGAAACAGGGCACTTGGTCTTTGGTACCTTGCACACCAGTTCAGCTGCCCAAACCGTTGAC
CGTATGGTGGATGTGTTCCCGCCAGAGCAGCAGCAGCAAATTCGCGTCCAGTTGTCCAACTCGTTAGTGGCGGTCTTTAG
CCAAACGCTGGTTCCTAAGAAAAATCCCAAGCCCGGTGAATTTGGGCGGATTATGGCACAAGAGATTATGGTAGTGACCC
CTGCTATCTCCAACCTGATTCGCGAAGGCAAGACCTCGCAGATTTATTCGGCAATTCAAACGGGCGGCAAGCTGGGGATG
CAGACCCTCGAAAAAGTCTTGGCCGATTATTATCGTGCGGGCATCATCACCTATGAAGCAGCAATGGCCAAGTCCTCGCG
TCAGGACGAGCTGCAACGTCTGATTGGTACGGGTACACCTGCGGCAGCAGCACGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(36-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.425

93.425

0.518
  pilT Legionella pneumophila strain ERS1305867

54.203

94.521

0.512
  pilT Legionella pneumophila strain Lp02

54.203

94.521

0.512
  pilT Pseudomonas stutzeri DSM 10701

53.936

93.973

0.507
  pilT Acinetobacter baylyi ADP1

54.572

92.877

0.507
  pilT Acinetobacter baumannii strain A118

53.412

92.329

0.493
  pilT Acinetobacter nosocomialis M2

53.412

92.329

0.493
  pilT Acinetobacter baumannii D1279779

53.412

92.329

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.286

95.89

0.482
  pilT Neisseria meningitidis 8013

49.284

95.616

0.471
  pilT Neisseria gonorrhoeae MS11

49.284

95.616

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

93.151

0.466
  pilT Vibrio cholerae strain A1552

50

93.151

0.466
  pilU Pseudomonas stutzeri DSM 10701

39.155

97.26

0.381
  pilU Acinetobacter baylyi ADP1

38.109

95.616

0.364