Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   M0644_RS02470 Genome accession   NZ_CP096573
Coordinates   499727..501751 (+) Length   674 a.a.
NCBI ID   WP_149817849.1    Uniprot ID   -
Organism   Thermosynechococcus sp. B1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 494727..506751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0644_RS02440 (M0644_02435) leuD 494940..495530 (+) 591 WP_286626447.1 3-isopropylmalate dehydratase small subunit -
  M0644_RS02445 (M0644_02440) - 495523..496479 (-) 957 WP_286626448.1 glycosyltransferase family 2 protein -
  M0644_RS02450 (M0644_02445) - 496570..497535 (+) 966 WP_286626449.1 hypothetical protein -
  M0644_RS02455 (M0644_02450) - 497532..499007 (-) 1476 WP_286626450.1 right-handed parallel beta-helix repeat-containing protein -
  M0644_RS02460 (M0644_02455) - 499055..499252 (-) 198 WP_024125597.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  M0644_RS02465 (M0644_02460) - 499253..499465 (-) 213 WP_286626452.1 hypothetical protein -
  M0644_RS02470 (M0644_02465) pilB/pilB1 499727..501751 (+) 2025 WP_149817849.1 GspE/PulE family protein Machinery gene
  M0644_RS02475 (M0644_02470) pilT 501819..502916 (+) 1098 WP_149817851.1 type IV pilus twitching motility protein PilT Machinery gene
  M0644_RS02480 (M0644_02475) - 502953..504173 (+) 1221 WP_286626454.1 type II secretion system F family protein -
  M0644_RS02485 (M0644_02480) - 504182..504655 (+) 474 WP_206201315.1 J domain-containing protein -
  M0644_RS02490 (M0644_02485) - 504652..504924 (+) 273 WP_149817855.1 DUF3143 domain-containing protein -
  M0644_RS02495 (M0644_02490) - 504921..505274 (-) 354 WP_149817857.1 hypothetical protein -
  M0644_RS02500 (M0644_02495) - 505569..506714 (-) 1146 WP_286626455.1 cysteine desulfurase family protein -

Sequence

Protein


Download         Length: 674 a.a.        Molecular weight: 74306.57 Da        Isoelectric Point: 5.5500

>NTDB_id=681810 M0644_RS02470 WP_149817849.1 499727..501751(+) (pilB/pilB1) [Thermosynechococcus sp. B1]
MVNTSSSSSRKALTVRGRAASPTERAIVASGYASIDQVREAMNTARKTGKSLVTVLQEITGSTMPPDVLRQYHKQQLFEL
KVIYGVDCLDPELNRFPTEQIEELINTIVPIDTCRTYQVIPIAKHLDADPPYLLVAMVDPDNLQAIDNLTRLLRAHNLTL
KRMVITLEDYQRLIDPILNKQVAETTAGKNAPAAIGDINIEEDIEAMGGLEELEGEQEVDLAEALKGAEDAPIIALVNKI
LAKALTEGVSDIHIEPQEEYLRIRFRKDGVLHQAFDPLPKKIVPAVVSRFKILADLDIAERRAPQDGRIRKMFQGRRVDF
RVNTLPSRWGEKVVLRILDNSATQLGLDKLITDPESLAIVREMTKRPFGLILVTGPTGSGKTTTLYSALAECNSPGVNIS
TAEDPIEYTLPGLTQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAVAR
LSEMGVEPFMVSASLIGVVAQRLMRRVCSECRIPYTPTREELARFGLSASKDVNLTLYKANKLTPEQIQAAKASGQPICS
KCGGVGYKGRVGVYEIMRVTERLQSLITEGAPTERIKEAAVEEGMKTLLAYSLNLVKEGVTTLEEVERVTFTDTGLESEL
KAKRKSSLTCRTCGAEAQPEWLECPYCMTPRFVD

Nucleotide


Download         Length: 2025 bp        

>NTDB_id=681810 M0644_RS02470 WP_149817849.1 499727..501751(+) (pilB/pilB1) [Thermosynechococcus sp. B1]
ATGGTCAACACATCGTCATCTTCTTCTCGGAAAGCACTTACCGTGCGCGGCAGAGCCGCTAGCCCCACAGAACGAGCCAT
TGTTGCCTCCGGTTACGCCAGTATCGATCAGGTGCGAGAAGCCATGAACACCGCCCGCAAAACGGGCAAGTCCCTCGTAA
CCGTGTTACAGGAGATTACCGGCAGCACCATGCCCCCCGATGTGTTACGCCAGTACCACAAGCAACAGCTTTTTGAACTC
AAAGTGATCTACGGCGTTGATTGCCTCGATCCCGAACTCAATCGTTTCCCCACCGAGCAAATCGAGGAACTGATCAACAC
GATTGTACCGATTGACACCTGCCGCACCTACCAAGTCATCCCCATTGCCAAGCACCTCGACGCTGATCCCCCCTATCTGC
TCGTGGCGATGGTGGATCCAGATAACCTCCAAGCCATTGACAACCTCACCCGCTTACTCCGCGCCCACAACCTCACCCTC
AAGCGAATGGTGATCACCCTAGAGGATTACCAACGCCTCATTGACCCGATTCTCAATAAACAGGTAGCTGAAACCACTGC
TGGCAAAAACGCCCCGGCTGCGATCGGTGACATCAACATCGAAGAAGACATCGAAGCCATGGGCGGCCTCGAAGAGCTGG
AAGGGGAGCAGGAGGTTGACCTTGCCGAGGCGCTGAAAGGCGCAGAAGATGCTCCGATTATTGCCCTTGTCAACAAAATT
TTGGCTAAAGCCCTCACCGAAGGCGTTTCCGACATTCACATCGAACCTCAAGAGGAATACCTGCGGATTCGTTTCCGCAA
AGATGGGGTGCTCCACCAAGCCTTTGATCCACTGCCGAAGAAAATCGTGCCAGCCGTCGTTTCCCGCTTCAAAATCTTGG
CTGACCTTGATATTGCCGAGCGGCGTGCCCCCCAAGATGGTCGTATCCGCAAAATGTTCCAAGGGCGACGGGTGGACTTC
CGAGTGAATACCCTCCCCAGCCGCTGGGGCGAAAAAGTCGTACTGCGGATTCTCGATAACTCCGCCACCCAACTCGGTCT
CGACAAGCTGATTACTGACCCTGAGAGCCTTGCTATTGTGCGGGAGATGACCAAGCGCCCCTTTGGCCTCATCCTCGTTA
CAGGTCCCACTGGTTCAGGGAAAACCACCACGCTTTACTCAGCGCTGGCTGAATGTAATAGTCCCGGTGTCAACATCAGT
ACAGCGGAAGACCCGATTGAATACACGCTCCCCGGCCTAACGCAGGTACAGGTTATTCGCGAAAAAGGCATGGACTTTGC
CTCCATTCTGCGTGCCTTTCTCCGCCAAGACCCCGATGTGATTCTGGTGGGGGAAACCCGCGACAAGGAAACAGCAAAAA
CCGCTATTGAAGCTGCCTTGACAGGTCACTTGGTGTTAACCACCCTGCACACCAACGATGCCGCCAGTGCCGTTGCCCGT
CTCTCGGAAATGGGGGTGGAACCCTTCATGGTCTCCGCTTCCTTAATTGGGGTCGTTGCCCAGCGACTGATGCGGCGGGT
ATGCAGTGAGTGTCGCATTCCCTATACGCCCACCCGTGAGGAACTGGCACGCTTTGGCTTGTCTGCCTCGAAGGATGTGA
ACCTCACCCTCTACAAAGCCAATAAACTCACACCCGAGCAGATTCAAGCAGCCAAAGCCAGTGGCCAGCCGATTTGTAGC
AAGTGTGGCGGTGTTGGCTACAAAGGGCGGGTCGGGGTCTACGAAATTATGCGGGTGACGGAGCGCCTGCAAAGCCTGAT
TACTGAGGGTGCCCCCACGGAGCGGATCAAGGAAGCGGCGGTTGAAGAGGGCATGAAAACCCTGTTGGCCTATAGCCTGA
ACCTTGTCAAAGAAGGGGTCACCACCCTCGAGGAAGTCGAGCGCGTCACCTTTACAGACACGGGTCTCGAATCAGAACTC
AAGGCCAAACGTAAGAGTTCTTTAACCTGCCGTACCTGCGGTGCCGAGGCGCAGCCCGAATGGTTAGAATGCCCCTATTG
CATGACACCCCGCTTTGTGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(74-178)

Type II/IV secretion system protein (T2SSE)

(239-506)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.549

97.329

0.638
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.148

94.51

0.361