Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M0M92_RS21820 Genome accession   NZ_CP096263
Coordinates   4503809..4504789 (-) Length   326 a.a.
NCBI ID   WP_049014209.1    Uniprot ID   -
Organism   Klebsiella quasipneumoniae strain KP20-558     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4498809..4509789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0M92_RS21790 (M0M92_21795) endA 4499100..4499807 (+) 708 WP_032454045.1 deoxyribonuclease I -
  M0M92_RS21795 (M0M92_21800) rsmE 4499899..4500630 (+) 732 WP_023290991.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  M0M92_RS21800 (M0M92_21805) gshB 4500651..4501601 (+) 951 WP_004205176.1 glutathione synthase -
  M0M92_RS21805 (M0M92_21810) - 4501776..4502339 (+) 564 WP_002916603.1 YqgE/AlgH family protein -
  M0M92_RS21810 (M0M92_21815) ruvX 4502339..4502755 (+) 417 WP_004205178.1 Holliday junction resolvase RuvX -
  M0M92_RS21815 (M0M92_21820) - 4502807..4503460 (-) 654 WP_044524701.1 LuxR C-terminal-related transcriptional regulator -
  M0M92_RS21820 (M0M92_21825) pilT 4503809..4504789 (-) 981 WP_049014209.1 type IV pilus twitching motility protein PilT Machinery gene
  M0M92_RS21825 (M0M92_21830) - 4504806..4505507 (+) 702 WP_004205181.1 YggS family pyridoxal phosphate-dependent enzyme -
  M0M92_RS21830 (M0M92_21835) - 4505528..4506094 (+) 567 WP_002916613.1 YggT family protein -
  M0M92_RS21835 (M0M92_21840) yggU 4506091..4506381 (+) 291 WP_004205183.1 DUF167 family protein YggU -
  M0M92_RS21840 (M0M92_21845) - 4506394..4506987 (+) 594 WP_023290995.1 XTP/dITP diphosphatase -
  M0M92_RS21845 (M0M92_21850) hemW 4506980..4508116 (+) 1137 WP_023319135.1 radical SAM family heme chaperone HemW -
  M0M92_RS21850 (M0M92_21855) - 4508421..4509407 (+) 987 WP_072421875.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35367.79 Da        Isoelectric Point: 6.8575

>NTDB_id=680759 M0M92_RS21820 WP_049014209.1 4503809..4504789(-) (pilT) [Klebsiella quasipneumoniae strain KP20-558]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLEAAPFPAPDIADLLNDWLDAAQFQHWQEHGQIDFALTLACGSRLRA
SAFAHTRGISLVLRLLPVQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFSAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGQL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=680759 M0M92_RS21820 WP_049014209.1 4503809..4504789(-) (pilT) [Klebsiella quasipneumoniae strain KP20-558]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGGCCGCTCCCTTTCCCGCGCCGGATATCGCGGATTTACTCAACGACTGGCTCGATG
CCGCACAGTTCCAGCACTGGCAGGAGCACGGCCAGATTGACTTTGCGCTGACTCTGGCCTGCGGTTCGCGGCTACGCGCC
AGCGCTTTCGCCCATACGCGCGGAATATCGCTGGTGCTGCGCCTGCTGCCCGTCCAGTGCCCGCGTCTGGAGACGCTGGG
CGCGCCGCCTGCGCTAAGTGAACTACTGGCGGAAGAGAGTGGCTTGCTGCTGGTCACCGGGGCGACGGGCAGCGGCAAGT
CGACTACCCTGGCGGCGATGGTCGGGCATCTCAACCAGCATCTTGATGGCCATATTCTGACGCTGGAGGATCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCAGCAGCGGGAGATTGGCCGTCACTGTCCTTCGTTCTCCGCGGCGCTTCG
GGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTTGGGGAGTTACGCGACAGCGAAACCATCCGTCTGGCGTTGACGG
CTGCGGAAACCGGGCATCTGGTGATGGCGACATTACACACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCAGAGGAGAAAGATCAGGTTCGGAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTATTACC
CGCACGTCAGGGCGGCAGAGTTGCGCTATACGAGCTTCTGGTCAATACGCCGGCGGTGGCGAATTTGATCCGCGAAGGAA
AAGTGCACCAGCTGCCCGGTGTTATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACCCAGAGTTTTCAGCAACGT
GTGGCCGCAGGCCAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.988

100

0.521
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.988

100

0.521
  pilT Acinetobacter baylyi ADP1

48.624

100

0.488
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

47.706

100

0.479
  pilT Legionella pneumophila strain ERS1305867

47.84

99.387

0.475
  pilT Legionella pneumophila strain Lp02

47.84

99.387

0.475
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Vibrio cholerae strain A1552

40.729

100

0.411
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.252

98.466

0.386