Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M0M42_RS19200 Genome accession   NZ_CP096208
Coordinates   4143350..4144384 (-) Length   344 a.a.
NCBI ID   WP_248494041.1    Uniprot ID   -
Organism   Pseudomonas knackmussii strain B09-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4138350..4149384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0M42_RS19180 (M0M42_19180) - 4139532..4139930 (-) 399 WP_222909776.1 TM2 domain-containing protein -
  M0M42_RS19185 (M0M42_19185) - 4140069..4141286 (+) 1218 WP_248494037.1 putative DNA modification/repair radical SAM protein -
  M0M42_RS19190 (M0M42_19190) - 4141286..4142125 (+) 840 WP_248494039.1 TIGR03915 family putative DNA repair protein -
  M0M42_RS19195 (M0M42_19195) pilU 4142126..4143271 (-) 1146 WP_045164002.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M0M42_RS19200 (M0M42_19200) pilT 4143350..4144384 (-) 1035 WP_248494041.1 type IV pilus twitching motility protein PilT Machinery gene
  M0M42_RS19205 (M0M42_19205) - 4144451..4145164 (+) 714 WP_248494043.1 YggS family pyridoxal phosphate-dependent enzyme -
  M0M42_RS19210 (M0M42_19210) proC 4145177..4146004 (+) 828 WP_248494045.1 pyrroline-5-carboxylate reductase -
  M0M42_RS19215 (M0M42_19215) - 4146281..4146871 (+) 591 WP_222909782.1 YggT family protein -
  M0M42_RS19220 (M0M42_19220) - 4146993..4148954 (+) 1962 WP_248494047.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37947.66 Da        Isoelectric Point: 6.8360

>NTDB_id=680429 M0M42_RS19200 WP_248494041.1 4143350..4144384(-) (pilT) [Pseudomonas knackmussii strain B09-2]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNSTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKRVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDSCL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=680429 M0M42_RS19200 WP_248494041.1 4143350..4144384(-) (pilT) [Pseudomonas knackmussii strain B09-2]
ATGGATATCACAGAGCTGCTGGCCTTCAGTGCCAAGCAGGGTGCATCGGATTTGCACCTGTCTTCCGGCTTGCCGCCGAT
GATCCGCGTCGATGGCGATGTACGCCGGATCAACCTCCCCGCGATGGACCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGTAAGGATTTCGAGGAATTTCTCGAGACCGACTTCTCGTTCGAAGTTCCCGGTGTCGCGCGT
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCTGGCGCGGTGTTCCGGACCATTCCGTCGAAGGTCCTGACCATGGA
AGACCTCGGGATGGGTGAGGTCTTCCGCAAGATCACCGATGTGCCGCGCGGCCTGGTGCTGGTGACAGGTCCAACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCCATGCTCGACTACCTCAACAGCACCAAGTATCACCACATTCTCACCATCGAAGAC
CCGATCGAATTCGTCCATGAATCGAAAAAGTGTCTGGTCAACCAACGCGAAGTGCACCGTGACACGCTCGGCTTCTCTGA
GGCCTTGCGCTCGGCTCTGCGGGAAGACCCGGACATCATCCTCGTCGGCGAAATGCGTGACCTGGAGACCATCCGTTTGG
CGCTGACTGCTGCAGAAACAGGCCACCTGGTGTTTGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGCGTA
GTTGACGTATTCCCGGCGGAAGAAAAATCCATGGTCCGCTCGATGCTTTCCGAGTCGCTGCAGGCGGTGATTTCCCAGAC
GTTGCTCAAGAGGGTCGGCGGTGGCCGCGTTGCGGCACACGAGATCATGATTGGCACGCCGGCGATCCGGAACCTGATCC
GTGAAGACAAAGTCGCTCAGATGTACTCTTCGATCCAGACCGGTGGTTCGTTGGGCATGCAAACGCTCGATTCCTGCCTA
AAAGGCCTGCTTGCCAAGGGCCTGATTTCACGCGATAGCGCAAAAGAAAAAGCCAAACAGCCAGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

99.419

100

0.994
  pilT Pseudomonas aeruginosa PAK

93.605

100

0.936
  pilT Acinetobacter baumannii D1279779

83.721

100

0.837
  pilT Acinetobacter baumannii strain A118

83.721

100

0.837
  pilT Acinetobacter nosocomialis M2

83.43

100

0.834
  pilT Acinetobacter baylyi ADP1

82.849

100

0.828
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392