Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MY052_RS03965 Genome accession   NZ_CP096201
Coordinates   823867..824847 (+) Length   326 a.a.
NCBI ID   WP_248538240.1    Uniprot ID   -
Organism   Kluyvera ascorbata strain SK     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 818867..829847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MY052_RS03930 (MY052_03930) - 819303..819629 (+) 327 WP_035892034.1 YggL family protein -
  MY052_RS03935 (MY052_03935) - 819682..820401 (+) 720 WP_035892033.1 DUF2884 domain-containing protein -
  MY052_RS03940 (MY052_03940) hemW 820543..821679 (-) 1137 WP_248538238.1 radical SAM family heme chaperone HemW -
  MY052_RS03945 (MY052_03945) - 821672..822265 (-) 594 WP_248538239.1 XTP/dITP diphosphatase -
  MY052_RS03950 (MY052_03950) yggU 822274..822564 (-) 291 WP_035892030.1 DUF167 family protein YggU -
  MY052_RS03955 (MY052_03955) - 822561..823127 (-) 567 WP_035892029.1 YggT family protein -
  MY052_RS03960 (MY052_03960) - 823146..823850 (-) 705 WP_123652548.1 YggS family pyridoxal phosphate-dependent enzyme -
  MY052_RS03965 (MY052_03965) pilT 823867..824847 (+) 981 WP_248538240.1 type IV pilus twitching motility protein PilT Machinery gene
  MY052_RS03970 (MY052_03970) - 825371..826024 (+) 654 WP_035892026.1 LuxR C-terminal-related transcriptional regulator -
  MY052_RS03975 (MY052_03975) ruvX 826126..826545 (-) 420 WP_035892025.1 Holliday junction resolvase RuvX -
  MY052_RS03980 (MY052_03980) - 826545..827108 (-) 564 WP_248538241.1 YqgE/AlgH family protein -
  MY052_RS03985 (MY052_03985) gshB 827254..828201 (-) 948 WP_035892023.1 glutathione synthase -
  MY052_RS03990 (MY052_03990) rsmE 828214..828945 (-) 732 WP_123652550.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MY052_RS03995 (MY052_03995) endA 829024..829731 (-) 708 WP_064542663.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35539.68 Da        Isoelectric Point: 6.5512

>NTDB_id=680278 MY052_RS03965 WP_248538240.1 823867..824847(+) (pilT) [Kluyvera ascorbata strain SK]
MDIQEMVSLSVKHNVSDLHLCSSGMARWRQAGQLLIAPFPSPEPQILLDAWLSETQRREWQQRQQLDFALSLAGCPGLRA
SAFAHSGGVSLVLRLLSTHCPSLDELHTPVALAELLREESGLILVTGSTGSGKSTTLAAMVDHLNHQLAGHILTLEDPVE
FLHESGSCLIHQREVGLHTSSFSEALRAALRQDPDVILLGELRDSDTIRLALTAAETGHLVLATLHTRGAAQAIERLIDV
FPAQEKDPVRNQLASSLCAVLSQKLQSDKHGGRVALFEMLINTPAVGNLIREGKTHQLAGVMQTGLQSGMQTFSQSYQQR
VKAWML

Nucleotide


Download         Length: 981 bp        

>NTDB_id=680278 MY052_RS03965 WP_248538240.1 823867..824847(+) (pilT) [Kluyvera ascorbata strain SK]
ATGGATATACAAGAAATGGTGTCGCTTAGTGTAAAGCATAACGTATCGGATCTACACCTATGTAGCAGCGGGATGGCACG
CTGGCGCCAGGCCGGTCAGCTGTTAATTGCGCCATTTCCGTCGCCTGAGCCGCAAATACTGCTGGATGCATGGTTAAGTG
AAACACAGCGGCGCGAGTGGCAGCAGCGCCAACAGCTTGATTTTGCGCTCTCCCTTGCCGGTTGCCCGGGCCTGAGGGCC
AGCGCTTTTGCCCATAGCGGCGGGGTTTCATTGGTACTGCGGCTGTTATCAACACATTGTCCGAGTCTTGATGAACTGCA
CACGCCGGTAGCACTCGCGGAGTTACTGCGAGAAGAGAGCGGGCTGATTCTGGTGACGGGGTCCACCGGTAGCGGCAAAT
CAACCACGCTCGCCGCGATGGTTGATCACCTCAATCATCAGCTTGCCGGGCATATTTTAACGCTTGAAGACCCCGTCGAA
TTTCTGCATGAAAGCGGAAGCTGTTTGATACATCAGCGGGAAGTCGGCCTGCACACCTCCTCATTTTCCGAGGCGCTACG
GGCAGCGCTACGTCAGGACCCCGATGTGATTCTACTGGGTGAGCTACGTGACAGCGACACCATCCGGCTTGCGCTAACGG
CTGCGGAAACCGGGCATCTGGTGCTGGCAACGCTGCACACGCGAGGGGCTGCTCAGGCTATCGAACGGTTGATTGACGTG
TTTCCCGCGCAGGAAAAAGATCCGGTGCGTAATCAACTGGCGAGCAGTTTATGCGCGGTGCTATCCCAAAAGCTGCAGTC
GGATAAACACGGAGGGCGCGTCGCGCTGTTTGAAATGCTGATTAACACGCCGGCCGTCGGCAACCTGATTCGTGAAGGTA
AGACGCATCAGCTTGCTGGCGTGATGCAAACCGGGTTACAGAGTGGGATGCAGACTTTTAGCCAGAGCTATCAACAAAGA
GTAAAAGCCTGGATGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.379

98.773

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.379

98.773

0.488
  pilT Neisseria meningitidis 8013

46.97

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.667

100

0.472
  pilT Acinetobacter baylyi ADP1

47.516

98.773

0.469
  pilT Legionella pneumophila strain ERS1305867

46.061

100

0.466
  pilT Legionella pneumophila strain Lp02

46.061

100

0.466
  pilT Pseudomonas aeruginosa PAK

47.188

98.16

0.463
  pilT Acinetobacter baumannii D1279779

46.729

98.466

0.46
  pilT Acinetobacter nosocomialis M2

46.729

98.466

0.46
  pilT Acinetobacter baumannii strain A118

46.729

98.466

0.46
  pilT Pseudomonas stutzeri DSM 10701

47.468

96.933

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.304

96.933

0.429
  pilU Pseudomonas stutzeri DSM 10701

40.938

98.16

0.402
  pilU Acinetobacter baylyi ADP1

39.385

99.693

0.393
  pilU Vibrio cholerae strain A1552

39.198

99.387

0.39