Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LGT36_RS04725 Genome accession   NZ_CP096184
Coordinates   975968..977095 (+) Length   375 a.a.
NCBI ID   WP_226097314.1    Uniprot ID   -
Organism   Demequina sp. TMPB413     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 970968..982095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGT36_RS04705 (LGT36_004705) ruvX 971490..971957 (+) 468 WP_226097311.1 Holliday junction resolvase RuvX -
  LGT36_RS04710 (LGT36_004710) mltG 971950..973068 (+) 1119 WP_226097312.1 endolytic transglycosylase MltG -
  LGT36_RS04715 (LGT36_004715) - 973047..973922 (+) 876 WP_370634355.1 shikimate dehydrogenase -
  LGT36_RS04720 (LGT36_004720) - 974002..975669 (+) 1668 WP_226097313.1 GspE/PulE family protein -
  LGT36_RS04725 (LGT36_004725) pilT 975968..977095 (+) 1128 WP_226097314.1 type IV pilus twitching motility protein PilT Machinery gene
  LGT36_RS04730 (LGT36_004730) - 977115..978344 (+) 1230 WP_226097315.1 type II secretion system F family protein -
  LGT36_RS04735 (LGT36_004735) - 978452..978850 (+) 399 WP_226097316.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LGT36_RS04740 (LGT36_004740) - 978946..979395 (+) 450 WP_226095343.1 hypothetical protein -
  LGT36_RS04745 (LGT36_004745) - 979392..980015 (+) 624 WP_226095342.1 hypothetical protein -
  LGT36_RS04750 (LGT36_004750) - 980033..981913 (+) 1881 WP_226095341.1 hypothetical protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 40954.81 Da        Isoelectric Point: 6.9309

>NTDB_id=680206 LGT36_RS04725 WP_226097314.1 975968..977095(+) (pilT) [Demequina sp. TMPB413]
MNAAPRQQRVGMSQEMQPGDLDISDALRMMLRTGASDLHLTTGAPPMVRLDGQLAALPGFDVLKPDALQRSLYSMLTQKQ
REKFEEDFELDFSHALVGEARFRVNIYRQRDSLGAAFRVIPYEIKPLEALGIPPTVSAFAGLARGLVLVTGPTGSGKSTT
LASLVDLANRSRSAHIMTVEDPIEFLHRHKRSLVNQREVGSDTHSFQAALRHVLRQDPDIILIGEMRDLETIQVALTAAE
TGHLVFATLHTQDAAQTIDRVIDVFPSGQQGQIRTQLATAVQGIVCQTLLRRADGPGRAAAVEVLIATPAIRNLIREGKT
HQIYTALQAGASLGMQTMDQNLAELVKHGTVSYENAVDVCHSVEEFKRLCGRASG

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=680206 LGT36_RS04725 WP_226097314.1 975968..977095(+) (pilT) [Demequina sp. TMPB413]
ATGAACGCCGCTCCCAGGCAGCAACGCGTCGGCATGTCTCAGGAGATGCAACCGGGTGACCTCGACATCTCGGACGCGTT
GAGGATGATGTTGCGCACCGGCGCATCCGACTTGCACTTGACTACTGGCGCGCCGCCCATGGTGCGTCTTGACGGTCAGC
TCGCGGCGTTGCCCGGCTTTGATGTGCTGAAGCCCGACGCGCTCCAGCGCTCGCTCTACTCGATGCTGACGCAAAAGCAG
AGGGAGAAGTTCGAGGAGGACTTCGAACTCGACTTCTCTCACGCCCTCGTAGGCGAGGCGCGCTTTCGCGTGAACATTTA
CCGCCAGCGCGACTCGCTTGGCGCGGCGTTCCGGGTGATTCCTTATGAGATCAAGCCGCTTGAGGCACTAGGGATCCCGC
CGACGGTCTCGGCCTTCGCTGGGCTCGCTCGCGGACTTGTGCTGGTCACGGGTCCCACGGGTTCTGGAAAGTCGACCACG
CTCGCGTCGCTGGTCGATCTTGCCAACCGGTCGCGCAGCGCCCACATCATGACAGTCGAGGACCCGATTGAGTTCCTTCA
CCGTCACAAGCGGAGTCTGGTCAACCAGCGTGAGGTCGGCTCGGACACCCATTCTTTCCAGGCGGCTTTGCGACATGTCC
TGCGCCAGGACCCCGACATCATCCTCATCGGCGAGATGCGCGACCTGGAGACGATCCAGGTCGCGTTGACTGCGGCAGAG
ACCGGACACCTCGTGTTCGCGACTCTTCACACGCAAGACGCCGCGCAAACGATCGACCGCGTGATTGACGTTTTCCCGTC
GGGGCAACAAGGTCAGATTCGCACCCAACTGGCGACAGCGGTACAAGGAATCGTTTGCCAGACGCTGCTCAGAAGGGCCG
ACGGTCCCGGCCGCGCAGCTGCGGTCGAGGTGCTCATCGCGACGCCAGCTATCAGGAACCTGATCCGCGAGGGCAAGACT
CACCAGATTTATACGGCGCTGCAAGCGGGGGCCAGTCTCGGCATGCAGACCATGGATCAGAACCTCGCGGAACTGGTCAA
ACATGGCACGGTGTCTTACGAGAACGCCGTTGACGTGTGCCACAGCGTCGAGGAATTCAAGCGGCTCTGTGGCCGTGCGT
CAGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(32-291)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.542

94.4

0.496
  pilT Pseudomonas aeruginosa PAK

52.874

92.8

0.491
  pilT Pseudomonas stutzeri DSM 10701

52.299

92.8

0.485
  pilT Acinetobacter baylyi ADP1

51.724

92.8

0.48
  pilT Acinetobacter nosocomialis M2

51.734

92.267

0.477
  pilT Acinetobacter baumannii D1279779

51.734

92.267

0.477
  pilT Acinetobacter baumannii strain A118

51.734

92.267

0.477
  pilT Legionella pneumophila strain Lp02

52.083

89.6

0.467
  pilT Legionella pneumophila strain ERS1305867

52.083

89.6

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.36

88.267

0.453
  pilT Vibrio cholerae strain A1552

51.36

88.267

0.453
  pilT Neisseria meningitidis 8013

48.512

89.6

0.435
  pilT Neisseria gonorrhoeae MS11

48.214

89.6

0.432
  pilU Vibrio cholerae strain A1552

40.73

94.933

0.387
  pilU Acinetobacter baylyi ADP1

41.471

90.667

0.376
  pilU Pseudomonas stutzeri DSM 10701

41.716

90.133

0.376