Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TTHT_RS08100 Genome accession   NZ_AP017470
Coordinates   1753487..1754620 (-) Length   377 a.a.
NCBI ID   WP_201327471.1    Uniprot ID   A0A7R6SZ13
Organism   Thermotomaculum hydrothermale strain AC55     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1748487..1759620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TTHT_RS08075 (TTHT_1686) - 1748606..1750438 (-) 1833 WP_201327466.1 cytochrome c3 family protein -
  TTHT_RS08080 (TTHT_1687) - 1750553..1751338 (-) 786 WP_201327467.1 thioesterase family protein -
  TTHT_RS08085 (TTHT_1688) - 1751319..1751777 (-) 459 WP_201327468.1 MauE/DoxX family redox-associated membrane protein -
  TTHT_RS08090 (TTHT_1689) - 1751711..1752184 (-) 474 WP_201327469.1 rhodanese-like domain-containing protein -
  TTHT_RS08095 (TTHT_1690) - 1752276..1753472 (-) 1197 WP_201327470.1 type II secretion system F family protein -
  TTHT_RS08100 (TTHT_1692) pilT 1753487..1754620 (-) 1134 WP_201327471.1 type IV pilus twitching motility protein PilT Machinery gene
  TTHT_RS08105 (TTHT_1693) pilB 1754658..1756379 (-) 1722 WP_201327472.1 type IV-A pilus assembly ATPase PilB -
  TTHT_RS08110 (TTHT_1694) glpX 1756460..1757437 (-) 978 WP_269089380.1 class II fructose-bisphosphatase -
  TTHT_RS08115 (TTHT_1695) - 1757450..1758619 (-) 1170 WP_201327473.1 acyl-CoA dehydrogenase family protein -
  TTHT_RS08120 (TTHT_1696) - 1758616..1759524 (-) 909 WP_201327474.1 outer membrane protein assembly factor BamD -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42071.98 Da        Isoelectric Point: 10.1139

>NTDB_id=67982 TTHT_RS08100 WP_201327471.1 1753487..1754620(-) (pilT) [Thermotomaculum hydrothermale strain AC55]
MDITLSQLLKKMVESGGTDLHITTNSPPQIRKDGKLIPLKMPPLTPAETKKLIYAIMTDAQKQKFEQDLEVDFSFGIKKL
ARFRANVFHQRGAVAGAFRLIPYEIRGFRELGIPMVVEKLCELPRGLVLVTGPTGSGKSTTLAAMIDKVNRERNAHIITI
EDPVEYLHSHKKCIVNQREVNTDTKSFSRALRAALRQDPDVVLIGEMRDLETVETALKIAETGHLTFATLHTNSAVQTIN
RIIDIFPAHQQQQIRTQLSFVLEGVICQTLLPKASGRGRVLALEILIPNAAVRNLIREDKIHQIYATMQTGQMKHGMQTF
NQSLASLFLQGLITKEVALAASSNKDELIDMINRGVGTVIEQPTQPTTKRNPKIKYT

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=67982 TTHT_RS08100 WP_201327471.1 1753487..1754620(-) (pilT) [Thermotomaculum hydrothermale strain AC55]
ATGGATATTACTCTATCGCAGTTGCTGAAAAAAATGGTTGAATCGGGAGGAACAGACCTTCACATAACAACCAATAGTCC
TCCTCAAATAAGAAAAGACGGGAAACTTATCCCCTTGAAAATGCCTCCTTTAACCCCGGCTGAAACAAAGAAATTAATTT
ACGCAATTATGACAGATGCTCAAAAACAAAAGTTTGAACAGGATTTAGAAGTTGACTTTTCATTTGGAATAAAGAAACTT
GCAAGGTTCAGGGCAAATGTGTTTCATCAAAGAGGTGCAGTTGCAGGTGCATTCAGGCTTATCCCGTATGAAATAAGGGG
GTTTAGAGAGCTTGGAATTCCAATGGTTGTTGAAAAATTGTGTGAGCTTCCCAGAGGGCTTGTGCTTGTTACAGGGCCAA
CAGGTTCAGGTAAATCAACCACTCTGGCGGCAATGATAGACAAAGTAAACAGAGAGAGAAATGCCCATATTATTACAATT
GAAGACCCGGTAGAGTATTTACATTCACATAAAAAGTGTATTGTTAATCAGAGGGAAGTCAATACAGACACAAAATCATT
TAGCAGGGCTTTAAGAGCAGCATTGAGACAGGATCCTGACGTCGTTCTTATTGGTGAGATGAGGGATTTAGAAACTGTTG
AAACAGCCTTAAAGATTGCGGAAACAGGCCATTTAACCTTTGCTACATTGCATACTAACTCGGCAGTTCAAACTATAAAC
AGGATTATAGATATTTTCCCTGCTCACCAGCAACAGCAGATAAGAACACAGCTTTCTTTTGTATTGGAAGGTGTAATTTG
TCAAACGCTTCTTCCCAAGGCTAGCGGTAGAGGAAGGGTTCTTGCACTGGAAATACTGATACCAAATGCGGCTGTGAGAA
ACTTGATTAGAGAGGACAAAATACATCAGATTTACGCTACAATGCAGACAGGTCAGATGAAACATGGTATGCAAACATTT
AACCAATCCCTTGCTTCACTGTTTTTGCAGGGATTGATTACAAAAGAGGTAGCTCTTGCAGCATCGTCAAATAAAGATGA
ATTAATTGATATGATAAATAGAGGTGTTGGTACTGTTATTGAACAGCCTACTCAACCCACTACAAAGAGAAATCCAAAAA
TTAAATATACATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-263)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7R6SZ13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

53.913

91.512

0.493
  pilT Legionella pneumophila strain ERS1305867

53.913

91.512

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.319

97.347

0.48
  pilT Acinetobacter baylyi ADP1

51.724

92.308

0.477
  pilT Pseudomonas aeruginosa PAK

52.663

89.655

0.472
  pilT Pseudomonas stutzeri DSM 10701

52.071

89.655

0.467
  pilT Acinetobacter baumannii strain A118

49.857

92.573

0.462
  pilT Acinetobacter nosocomialis M2

49.857

92.573

0.462
  pilT Acinetobacter baumannii D1279779

49.857

92.573

0.462
  pilT Neisseria meningitidis 8013

49.419

91.247

0.451
  pilT Neisseria gonorrhoeae MS11

48.837

91.247

0.446
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.45

88.329

0.446
  pilT Vibrio cholerae strain A1552

50.45

88.329

0.446
  pilU Pseudomonas stutzeri DSM 10701

42.265

96.021

0.406
  pilU Vibrio cholerae strain A1552

42.486

91.777

0.39
  pilU Acinetobacter baylyi ADP1

39.943

92.308

0.369

Multiple sequence alignment