Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M0D46_RS11745 Genome accession   NZ_CP096143
Coordinates   2703844..2704977 (-) Length   377 a.a.
NCBI ID   WP_260813554.1    Uniprot ID   -
Organism   Xanthomonas prunicola strain CIX97     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2698844..2709977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0D46_RS11720 (M0D46_11710) - 2698941..2699276 (-) 336 WP_252162012.1 phasin family protein -
  M0D46_RS11725 (M0D46_11715) queD 2699469..2699822 (-) 354 WP_101362487.1 6-carboxytetrahydropterin synthase QueD -
  M0D46_RS11730 (M0D46_11720) - 2699940..2701325 (-) 1386 WP_101362151.1 DEAD/DEAH box helicase -
  M0D46_RS11735 (M0D46_11725) - 2701636..2702694 (+) 1059 WP_260808277.1 fumarylacetoacetate hydrolase family protein -
  M0D46_RS11740 (M0D46_11730) maiA 2702738..2703400 (+) 663 WP_260807319.1 maleylacetoacetate isomerase -
  M0D46_RS11745 (M0D46_11735) pilU 2703844..2704977 (-) 1134 WP_260813554.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M0D46_RS11750 (M0D46_11740) - 2705076..2705459 (+) 384 WP_010371890.1 DUF4398 domain-containing protein -
  M0D46_RS11755 (M0D46_11745) - 2705456..2706346 (+) 891 WP_252162015.1 hypothetical protein -
  M0D46_RS11760 (M0D46_11750) - 2706698..2706916 (+) 219 WP_003484103.1 YdcH family protein -
  M0D46_RS11765 (M0D46_11755) - 2707156..2708526 (+) 1371 WP_252162016.1 pyridoxal-phosphate dependent enzyme -
  M0D46_RS11770 (M0D46_11760) - 2708615..2709811 (+) 1197 WP_260807322.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41793.14 Da        Isoelectric Point: 6.1401

>NTDB_id=679684 M0D46_RS11745 WP_260813554.1 2703844..2704977(-) (pilU) [Xanthomonas prunicola strain CIX97]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYEVASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=679684 M0D46_RS11745 WP_260813554.1 2703844..2704977(-) (pilU) [Xanthomonas prunicola strain CIX97]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTATCCGCTCGGCAATACGGGGCTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCCGACGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTTGGTTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCATGCCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCAGAGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGTCACCTGTGCCTGGCGACGCTGCACTCCAACAATGCCGACCAGACCATCGAACGCATC
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAATCTGGCGCTGAATCTGCGCGCAGTGGTCTCGCAGCG
CCTGGTCAAGGGCGTGGACGGACGCCGCCGCCCCGCCACCGAGGTGCTGCTCAATACCCCGATGATCCGCGACCTGCTGC
GGCGCGGCCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAATCGCTGGAAGAAGGCATGCAAACCTTCGACCAATGTCTG
TTCCGCATGGTCAAATCCGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCTGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTCTCCGAAGGCGGCTCCGGCGAACACGACCCGTATGCGGATTACGAAGTGGCCTCGGCGCCGAAGATCAGTC
ACGGGTTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.503

100

0.451
  pilT Pseudomonas aeruginosa PAK

42.899

89.655

0.385
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Vibrio cholerae strain A1552

41.141

88.329

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.329

0.363
  pilT Neisseria gonorrhoeae MS11

39.193

92.042

0.361
  pilT Neisseria meningitidis 8013

39.193

92.042

0.361