Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CPY98_RS02230 Genome accession   NZ_AP017469
Coordinates   484892..485908 (-) Length   338 a.a.
NCBI ID   WP_096386197.1    Uniprot ID   -
Organism   Hafnia sp. CBA7124     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 479892..490908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPY98_RS02190 - 479940..480473 (+) 534 WP_096388704.1 SprT family zinc-dependent metalloprotease -
  CPY98_RS02195 endA 480603..481313 (+) 711 WP_043490630.1 deoxyribonuclease I -
  CPY98_RS02200 rsmE 481436..482167 (+) 732 WP_096386194.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CPY98_RS02205 gshB 482177..483130 (+) 954 WP_040046342.1 glutathione synthase -
  CPY98_RS02210 - 483338..483901 (+) 564 WP_043490633.1 YqgE/AlgH family protein -
  CPY98_RS02215 ruvX 483901..484323 (+) 423 WP_046458214.1 Holliday junction resolvase RuvX -
  CPY98_RS02220 - 484329..484583 (-) 255 WP_025798702.1 Txe/YoeB family addiction module toxin -
  CPY98_RS02225 - 484576..484827 (-) 252 WP_025798704.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  CPY98_RS02230 pilT 484892..485908 (-) 1017 WP_096386197.1 type IV pilus twitching motility protein PilT Machinery gene
  CPY98_RS02235 - 485931..486644 (+) 714 WP_096386199.1 YggS family pyridoxal phosphate-dependent enzyme -
  CPY98_RS02240 - 486718..487272 (+) 555 WP_025798710.1 YggT family protein -
  CPY98_RS02245 yggU 487278..487580 (+) 303 WP_025798712.1 DUF167 family protein YggU -
  CPY98_RS02250 rdgB 487634..488227 (+) 594 WP_043490641.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  CPY98_RS02255 hemW 488220..489350 (+) 1131 WP_046359444.1 radical SAM family heme chaperone HemW -
  CPY98_RS02260 epmB 489411..490445 (-) 1035 WP_043490645.1 EF-P beta-lysylation protein EpmB -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37091.49 Da        Isoelectric Point: 6.7216

>NTDB_id=67960 CPY98_RS02230 WP_096386197.1 484892..485908(-) (pilT) [Hafnia sp. CBA7124]
MDFCDLLARSVKQNASDLHLCTGYPPILRIDGSLQTIEAEPITSRQIDSWLAMHLPTEADTLWRQHHQVDFALNLHGGIR
LRVNAFQQLHGPSLALRIIPNTIPTLHDLSAPAALPHLLEVGSGLILVCGATGSGKSTTLAAMLNHINHHQPWHVITLED
PLEFIHIPQQSLIQQREIGAHVPSFNQALRAALREDPDVILLGELRDAESIRLALTAAETGHLVLATLHTRGAAQAVERL
IDVFPAEEKNFVRTQIAGSLQAVLAQQLLPKQGGGRVAAFEFLLNTPAVSNLIREEKAHQIATVLQTGQRQGMLSFEQDI
ARLNAQGILGRIKNDHNH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=67960 CPY98_RS02230 WP_096386197.1 484892..485908(-) (pilT) [Hafnia sp. CBA7124]
ATGGATTTTTGTGATTTATTGGCTCGTAGTGTAAAGCAAAACGCATCAGATCTGCATCTTTGCACTGGATATCCGCCCAT
TCTTCGTATCGACGGCTCACTGCAAACAATAGAAGCTGAGCCGATAACATCGCGCCAGATTGACTCTTGGCTTGCGATGC
ATCTGCCCACCGAGGCCGATACCCTATGGCGACAGCATCATCAGGTCGATTTCGCCTTAAACCTACATGGGGGCATCCGG
CTCAGAGTCAATGCTTTCCAGCAATTACATGGCCCCTCTCTGGCCCTGCGCATTATTCCAAACACGATCCCGACGCTGCA
TGATTTATCTGCGCCAGCGGCACTCCCCCATCTCCTTGAGGTTGGCTCAGGACTCATCTTGGTTTGTGGTGCAACCGGCA
GCGGCAAATCCACAACGCTTGCCGCCATGCTGAACCATATAAACCACCATCAGCCATGGCACGTTATTACGCTTGAAGAT
CCTTTGGAGTTTATTCATATCCCGCAGCAATCGCTTATCCAGCAGCGTGAAATTGGCGCTCATGTTCCCAGCTTTAACCA
AGCGCTACGCGCCGCGTTGCGAGAGGATCCCGATGTCATTTTACTGGGTGAATTACGCGATGCGGAATCCATTCGTTTAG
CCCTCACGGCAGCAGAAACGGGCCATCTCGTATTAGCGACGCTACACACGCGAGGTGCCGCGCAAGCGGTAGAACGGCTG
ATTGATGTCTTTCCTGCCGAAGAGAAAAATTTTGTACGCACGCAAATCGCAGGCAGCCTGCAAGCGGTGCTGGCACAGCA
GCTTTTGCCCAAACAGGGCGGAGGAAGAGTTGCCGCGTTTGAGTTTCTGCTCAATACTCCGGCCGTCAGTAACTTAATCC
GTGAAGAAAAAGCGCACCAGATCGCCACCGTCCTACAAACGGGACAACGGCAAGGAATGCTAAGTTTTGAGCAGGATATT
GCTCGACTAAACGCACAAGGAATACTTGGGAGGATAAAAAATGACCATAACCACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

49.245

97.929

0.482
  pilT Vibrio cholerae strain A1552

49.544

97.337

0.482
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

97.337

0.482
  pilT Neisseria gonorrhoeae MS11

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

48.024

97.337

0.467
  pilT Pseudomonas stutzeri DSM 10701

47.13

97.929

0.462
  pilT Acinetobacter baumannii D1279779

47.112

97.337

0.459
  pilT Acinetobacter baumannii strain A118

47.112

97.337

0.459
  pilT Acinetobacter nosocomialis M2

46.809

97.337

0.456
  pilT Pseudomonas aeruginosa PAK

46.526

97.929

0.456
  pilT Legionella pneumophila strain Lp02

46.526

97.929

0.456
  pilT Legionella pneumophila strain ERS1305867

46.526

97.929

0.456
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.519

95.858

0.417
  pilU Acinetobacter baylyi ADP1

40.923

96.154

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.024

97.041

0.379
  pilU Vibrio cholerae strain A1552

38.272

95.858

0.367

Multiple sequence alignment