Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MZN67_RS11640 Genome accession   NZ_CP096116
Coordinates   2609068..2610189 (-) Length   373 a.a.
NCBI ID   WP_011216215.1    Uniprot ID   Q5WUC5
Organism   Legionella pneumophila subsp. pneumophila strain DFS-2111020037     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2604068..2615189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MZN67_RS11625 (MZN67_11595) - 2604950..2605387 (-) 438 WP_015444141.1 hypothetical protein -
  MZN67_RS11630 (MZN67_11600) - 2605668..2606963 (+) 1296 WP_374152041.1 serine/threonine transporter -
  MZN67_RS11635 (MZN67_11605) ankK 2607056..2608981 (-) 1926 WP_374152042.1 Dot/Icm T4SS effector AnkK/LegA5 -
  MZN67_RS11640 (MZN67_11610) pilU 2609068..2610189 (-) 1122 WP_011216215.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MZN67_RS11645 (MZN67_11615) - 2610279..2612207 (-) 1929 WP_038839261.1 L-gulono-gamma-lactone oxidase -
  MZN67_RS11650 (MZN67_11620) - 2612495..2613331 (-) 837 WP_038839263.1 phytanoyl-CoA dioxygenase family protein -
  MZN67_RS11655 (MZN67_11625) radA 2613328..2614683 (-) 1356 WP_027265192.1 DNA repair protein RadA -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41673.95 Da        Isoelectric Point: 5.4724

>NTDB_id=679472 MZN67_RS11640 WP_011216215.1 2609068..2610189(-) (pilU) [Legionella pneumophila subsp. pneumophila strain DFS-2111020037]
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKSNQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGR
FRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDDIKEAMARSTEQGMQTFDQAL
FNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKSFNIQDENLQIQPDDIDKV

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=679472 MZN67_RS11640 WP_011216215.1 2609068..2610189(-) (pilU) [Legionella pneumophila subsp. pneumophila strain DFS-2111020037]
ATGGATATTACTCCTTTTTTTAAGCTCATGGTCGATAAAGGTGCCTCAGATTTGTTTTTCAGTGTCGGGGCACCACCAAA
TATTAAAATCGAGGGCATCACATCACCTATTGGCCAGGTTCCTATCAAATCGAATCAAATGGCAGAAATTGTTTTATCTA
TTATGAATGACGCACAGCAAAAAGAATTTGAGTCTAAAATGGAACTCAATATGGCTTTTTCATTAGCTGGAGTAGGGCGT
TTCCGAATCAATTTATTCAGGCAGCGTGGCGAAGTGGCTATGGTGGTGCGTTACATCAAGGGCGAAATACCTTCTATAGA
AGATTTAAATCTTCCTCAAATTTTAAAGTCTCTGGTCTTGGAGCTGCGAGGTTTAGTATTGGTTGTTGGGTCCACGGGAT
CGGGTAAATCAACGACTCTGGCTGCGATGATCAACCACCGTAATGAAAATCACAGAGGGCATATTTTAACAATAGAGGAT
CCTATTGAATATATACATCAACATAAAAAATCAATAGTTGATCAACGAGAAATAGGTATTGATACTCTAAGCTATGACAA
TGCATTGAAAAACGCAATGAGAGAAGCGCCTGATGTGATATTAATTGGTGAAATTCGTGATCTTAACTCAATGAAACATG
CGATAGCTTATGCTGAAACGGGACATTTGTGTATTTCTACTTTGCATGCCAATAACGCCAATCAGGCTCTGGATAGAGTG
ATTAATTTTTTCCCCGATGAGGCAAGACAACAGATATTATTAGATTTATCTCTTAATTTAAAAGCAATCATTTCATTACG
TTTGATACCAGGAGTTGATAATCAACGTGTCGCAGCGGTGGAGATCTTGATTAACTCTCCTTACATTTCCGATTTGATTG
AAAAAGGGAAGATAGACGATATTAAAGAAGCCATGGCAAGAAGCACGGAACAAGGCATGCAGACTTTCGATCAGGCTTTA
TTTAACTTATATAAAGAAGGAAAAATTACCAAAGAAAATGCGATTAAGTATGCTGATTCAAAAAATAATGTGGGTTTACA
AATTCGATTGGGCGAAGAAAAAAGCTTTAATATTCAAGATGAGAATTTACAAATACAACCCGATGATATTGATAAGGTTT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5WUC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.367

94.906

0.525
  pilU Acinetobacter baylyi ADP1

52.958

95.174

0.504
  pilU Vibrio cholerae strain A1552

48.286

93.834

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.133

90.885

0.41
  pilT Legionella pneumophila strain Lp02

43.275

91.689

0.397
  pilT Legionella pneumophila strain ERS1305867

43.275

91.689

0.397
  pilT Pseudomonas aeruginosa PAK

42.857

91.957

0.394
  pilT Neisseria meningitidis 8013

42.075

93.029

0.391
  pilT Neisseria gonorrhoeae MS11

42.075

93.029

0.391
  pilT Acinetobacter nosocomialis M2

43.413

89.544

0.389
  pilT Acinetobacter baumannii D1279779

43.413

89.544

0.389
  pilT Acinetobacter baumannii strain A118

43.413

89.544

0.389
  pilT Pseudomonas stutzeri DSM 10701

41.983

91.957

0.386
  pilT Acinetobacter baylyi ADP1

40.882

91.153

0.373
  pilT Vibrio cholerae strain A1552

41.945

88.204

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.945

88.204

0.37