Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K8O62_RS12955 Genome accession   NZ_CP096041
Coordinates   2794889..2795941 (-) Length   350 a.a.
NCBI ID   WP_003386380.1    Uniprot ID   A0A2I4MRH7
Organism   Clostridium botulinum strain FE9504ACG     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2796858..2843663 2794889..2795941 flank 917


Gene organization within MGE regions


Location: 2794889..2843663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O62_RS12955 (K8O62_002570) pilT 2794889..2795941 (-) 1053 WP_003386380.1 type IV pilus twitching motility protein PilT Machinery gene
  K8O62_RS12960 (K8O62_002571) aroE 2795943..2796719 (-) 777 WP_004440850.1 shikimate dehydrogenase -
  K8O62_RS12965 (K8O62_002572) - 2796858..2797142 (-) 285 Protein_2557 sigma factor-like helix-turn-helix DNA-binding protein -
  K8O62_RS12970 (K8O62_002573) - 2797265..2797783 (-) 519 WP_071647417.1 hypothetical protein -
  K8O62_RS12975 (K8O62_002574) - 2797969..2798214 (-) 246 WP_071647418.1 hypothetical protein -
  K8O62_RS12980 (K8O62_002575) - 2798371..2798532 (+) 162 WP_154695484.1 hypothetical protein -
  K8O62_RS12985 (K8O62_002576) - 2798563..2799330 (-) 768 WP_071647419.1 N-acetylmuramoyl-L-alanine amidase -
  K8O62_RS12990 (K8O62_002577) - 2799372..2799566 (-) 195 WP_014521308.1 hypothetical protein -
  K8O62_RS12995 (K8O62_002578) - 2799580..2799834 (-) 255 WP_071647420.1 hemolysin XhlA family protein -
  K8O62_RS13000 (K8O62_002579) - 2799871..2800893 (-) 1023 WP_080488628.1 DNA cytosine methyltransferase -
  K8O62_RS13005 (K8O62_002580) - 2801072..2802274 (-) 1203 WP_003356357.1 IS110 family transposase -
  K8O62_RS13010 (K8O62_002581) - 2802590..2802712 (-) 123 WP_003400227.1 XkdX family protein -
  K8O62_RS13015 (K8O62_002582) - 2802705..2803070 (-) 366 WP_071647421.1 hypothetical protein -
  K8O62_RS13020 (K8O62_002583) - 2803083..2804912 (-) 1830 WP_071647422.1 PQQ-binding-like beta-propeller repeat protein -
  K8O62_RS13025 (K8O62_002584) - 2804920..2806077 (-) 1158 WP_071647423.1 siphovirus ReqiPepy6 Gp37-like family protein -
  K8O62_RS13030 (K8O62_002585) - 2806093..2806245 (-) 153 WP_155119189.1 hypothetical protein -
  K8O62_RS13035 (K8O62_002586) - 2806273..2807148 (-) 876 WP_071647424.1 phage tail domain-containing protein -
  K8O62_RS13040 (K8O62_002587) - 2807164..2810226 (-) 3063 WP_071647425.1 phage tail tape measure protein -
  K8O62_RS13045 (K8O62_002588) - 2810227..2810457 (-) 231 WP_205609420.1 hypothetical protein -
  K8O62_RS13050 (K8O62_002589) - 2810538..2810849 (-) 312 WP_071647426.1 hypothetical protein -
  K8O62_RS13055 (K8O62_002590) - 2810862..2811383 (-) 522 WP_071647427.1 hypothetical protein -
  K8O62_RS13060 (K8O62_002591) - 2811380..2811748 (-) 369 WP_071647428.1 hypothetical protein -
  K8O62_RS13065 (K8O62_002592) - 2811769..2812185 (-) 417 WP_071647429.1 hypothetical protein -
  K8O62_RS13070 (K8O62_002593) - 2812178..2812501 (-) 324 WP_071647430.1 hypothetical protein -
  K8O62_RS13075 (K8O62_002594) - 2812501..2812797 (-) 297 WP_071647431.1 phage head-tail connector protein -
  K8O62_RS13080 (K8O62_002595) - 2812781..2813071 (-) 291 WP_071647432.1 Rho termination factor N-terminal domain-containing protein -
  K8O62_RS13085 (K8O62_002596) - 2813087..2814070 (-) 984 WP_071647433.1 major capsid protein -
  K8O62_RS13090 (K8O62_002597) - 2814092..2814640 (-) 549 WP_071647434.1 phage scaffolding protein -
  K8O62_RS13095 (K8O62_002598) - 2814770..2815135 (-) 366 WP_071647435.1 ABC transporter ATPase -
  K8O62_RS13100 (K8O62_002599) - 2815201..2815422 (-) 222 WP_071647436.1 hypothetical protein -
  K8O62_RS13105 (K8O62_002600) - 2815424..2816608 (-) 1185 WP_080488630.1 minor capsid protein -
  K8O62_RS13110 (K8O62_002601) - 2816598..2818025 (-) 1428 WP_071647437.1 phage portal protein -
  K8O62_RS13115 (K8O62_002602) - 2818038..2819300 (-) 1263 WP_371316048.1 PBSX family phage terminase large subunit -
  K8O62_RS13120 (K8O62_002603) - 2819290..2819730 (-) 441 WP_071647438.1 terminase small subunit -
  K8O62_RS13125 (K8O62_002604) - 2819830..2820021 (-) 192 WP_071647439.1 hypothetical protein -
  K8O62_RS13130 (K8O62_002605) - 2820208..2820645 (-) 438 WP_071647440.1 siderophore-interacting protein -
  K8O62_RS13135 (K8O62_002606) - 2821022..2821189 (-) 168 WP_155119190.1 hypothetical protein -
  K8O62_RS13140 (K8O62_002607) - 2821201..2821392 (-) 192 WP_071647441.1 hypothetical protein -
  K8O62_RS13145 (K8O62_002608) - 2821430..2823295 (-) 1866 WP_071647442.1 phage/plasmid primase, P4 family -
  K8O62_RS13150 (K8O62_002609) - 2823501..2823656 (+) 156 WP_155119191.1 hypothetical protein -
  K8O62_RS13155 (K8O62_002610) - 2823863..2824192 (-) 330 WP_071647443.1 nucleoside triphosphate pyrophosphohydrolase family protein -
  K8O62_RS13160 (K8O62_002611) - 2824193..2824471 (-) 279 WP_071647956.1 DUF1599 domain-containing protein -
  K8O62_RS13165 (K8O62_002612) - 2824678..2824827 (-) 150 WP_192816370.1 hypothetical protein -
  K8O62_RS13170 (K8O62_002613) - 2824931..2825344 (-) 414 WP_071647444.1 hypothetical protein -
  K8O62_RS13175 (K8O62_002614) - 2825551..2827245 (-) 1695 WP_071647445.1 hypothetical protein -
  K8O62_RS13180 (K8O62_002615) - 2827245..2827433 (-) 189 WP_014521346.1 DUF6877 family protein -
  K8O62_RS13185 (K8O62_002616) - 2827487..2827942 (-) 456 WP_071647446.1 DUF669 domain-containing protein -
  K8O62_RS13190 (K8O62_002617) - 2827943..2828092 (-) 150 WP_155119192.1 hypothetical protein -
  K8O62_RS13195 (K8O62_002618) - 2828092..2829783 (-) 1692 WP_071647447.1 AAA family ATPase -
  K8O62_RS13200 (K8O62_002619) - 2829829..2830125 (-) 297 WP_071647957.1 VRR-NUC domain-containing protein -
  K8O62_RS13205 (K8O62_002620) - 2830122..2830304 (-) 183 WP_071647448.1 hypothetical protein -
  K8O62_RS13210 (K8O62_002621) - 2830322..2830696 (-) 375 WP_071647449.1 hypothetical protein -
  K8O62_RS13215 (K8O62_002622) - 2830697..2831830 (-) 1134 WP_071647450.1 DEAD/DEAH box helicase -
  K8O62_RS13220 (K8O62_002623) - 2831980..2832543 (-) 564 WP_080488633.1 NUMOD4 domain-containing protein -
  K8O62_RS13225 (K8O62_002624) - 2832549..2832719 (-) 171 WP_196220039.1 hypothetical protein -
  K8O62_RS13230 (K8O62_002625) - 2832975..2833172 (-) 198 WP_014521358.1 helix-turn-helix transcriptional regulator -
  K8O62_RS13235 (K8O62_002626) - 2833394..2833837 (+) 444 WP_071647451.1 helix-turn-helix domain-containing protein -
  K8O62_RS13240 (K8O62_002627) - 2833857..2834324 (+) 468 WP_071647452.1 ImmA/IrrE family metallo-endopeptidase -
  K8O62_RS13245 (K8O62_002628) - 2834614..2836131 (+) 1518 WP_071647453.1 recombinase family protein -
  K8O62_RS13250 (K8O62_002629) - 2836151..2836579 (-) 429 Protein_2614 sigma-70 family RNA polymerase sigma factor -
  K8O62_RS13255 (K8O62_002630) - 2837005..2837184 (-) 180 WP_003386384.1 aspartyl-phosphate phosphatase Spo0E family protein -
  K8O62_RS13260 (K8O62_002631) - 2837629..2837766 (-) 138 WP_003386386.1 hypothetical protein -
  K8O62_RS13265 (K8O62_002632) - 2838157..2838411 (+) 255 WP_003386388.1 hypothetical protein -
  K8O62_RS19315 - 2838618..2838917 (-) 300 Protein_2618 hypothetical protein -
  K8O62_RS13275 (K8O62_002634) - 2839503..2840171 (-) 669 WP_004440648.1 YsnF/AvaK domain-containing protein -
  K8O62_RS13280 (K8O62_002635) - 2840250..2840726 (-) 477 WP_080488634.1 YsnF/AvaK domain-containing protein -
  K8O62_RS13285 (K8O62_002636) - 2840853..2841053 (-) 201 WP_004440442.1 hypothetical protein -
  K8O62_RS19425 - 2841172..2841285 (-) 114 WP_071647960.1 DnaD domain protein -
  K8O62_RS13290 (K8O62_002637) - 2841781..2842065 (-) 285 WP_004440932.1 hypothetical protein -
  K8O62_RS13295 (K8O62_002638) - 2842076..2842879 (-) 804 WP_003397153.1 PD-(D/E)XK nuclease-like domain-containing protein -
  K8O62_RS13300 (K8O62_002639) - 2842860..2843663 (-) 804 WP_004440738.1 recombinase RecT -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39054.11 Da        Isoelectric Point: 8.0876

>NTDB_id=679359 K8O62_RS12955 WP_003386380.1 2794889..2795941(-) (pilT) [Clostridium botulinum strain FE9504ACG]
MKQLKELLEKTINFGASDLHLSVGVEPTIRINGQLSKIFKEKLTPEDTNNYAKEILGEEFEDLLKKGEIDTSYSISGLGR
FRVSVFKQRGSIAISLRVVSLKIPNLNELKLPDIVKELTNKKRGLILVTGPTGSGKSTTLAAMINEINNTRSNHIITLED
PIEFLHKHNKCIINQREIGRDTFSYKSALKAALREDPDVILIGELRDLEDISIALTAAETGHLVFSTLHTIGGAKTIDRI
VNVFPPHQQEQIRIQVASVLEGIISQQLVHKIDGGRIAALETMVTTNAIRNMIREGKTYQIESSIQTGSKYGMKTMDMSL
AELYKREIISYDTALNYSVDKDLITKIINL

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=679359 K8O62_RS12955 WP_003386380.1 2794889..2795941(-) (pilT) [Clostridium botulinum strain FE9504ACG]
TTGAAACAATTAAAAGAATTATTGGAAAAAACTATTAACTTTGGTGCTTCAGATTTACATTTATCCGTAGGGGTAGAACC
AACTATAAGAATAAATGGACAATTAAGTAAAATATTTAAAGAAAAGCTTACACCAGAAGATACTAATAATTATGCCAAAG
AAATATTAGGTGAAGAATTTGAAGATTTATTGAAAAAAGGAGAAATAGATACATCCTATTCTATATCGGGCCTAGGTAGA
TTCAGAGTAAGCGTGTTTAAACAAAGAGGAAGTATAGCTATTTCTTTAAGGGTTGTAAGTTTGAAGATCCCTAACTTAAA
TGAACTAAAATTGCCTGATATAGTAAAGGAATTAACCAATAAAAAAAGAGGCCTTATTTTAGTAACGGGTCCTACAGGTT
CAGGCAAAAGCACTACTTTAGCAGCTATGATTAATGAAATTAATAATACTAGGTCTAATCATATAATAACTTTAGAAGAT
CCAATAGAATTTTTACATAAACATAACAAATGTATAATAAATCAAAGAGAGATAGGTAGGGATACTTTTAGTTATAAAAG
TGCTTTAAAAGCAGCATTAAGAGAAGATCCGGATGTAATATTAATAGGAGAGCTTAGAGATTTAGAAGATATTTCTATAG
CTTTAACAGCTGCAGAAACAGGACATTTAGTATTTTCTACTTTGCATACTATAGGAGGGGCTAAAACTATAGATAGAATT
GTTAATGTATTTCCGCCACATCAACAGGAGCAGATAAGAATACAAGTAGCAAGTGTGTTAGAAGGAATAATAAGTCAACA
ATTAGTACATAAAATTGATGGAGGAAGAATAGCAGCCTTAGAGACTATGGTAACTACAAATGCCATAAGAAATATGATAA
GGGAAGGAAAAACTTATCAAATAGAATCTTCCATACAAACTGGATCTAAATATGGAATGAAAACTATGGATATGTCCTTA
GCAGAGCTATATAAAAGAGAAATTATAAGCTACGATACTGCATTAAATTATTCAGTAGATAAGGATTTAATTACAAAAAT
AATTAATTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(92-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I4MRH7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

50.751

95.143

0.483
  pilT Legionella pneumophila strain Lp02

48.696

98.571

0.48
  pilT Legionella pneumophila strain ERS1305867

48.696

98.571

0.48
  pilT Pseudomonas aeruginosa PAK

49.405

96

0.474
  pilT Acinetobacter nosocomialis M2

49.55

95.143

0.471
  pilT Acinetobacter baumannii D1279779

49.55

95.143

0.471
  pilT Acinetobacter baumannii strain A118

49.55

95.143

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

94.286

0.471
  pilT Vibrio cholerae strain A1552

50

94.286

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.81

96

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.264

99.429

0.46
  pilT Neisseria meningitidis 8013

47.463

95.714

0.454
  pilT Neisseria gonorrhoeae MS11

47.463

95.714

0.454
  pilU Acinetobacter baylyi ADP1

41.124

96.571

0.397
  pilU Pseudomonas stutzeri DSM 10701

41.194

95.714

0.394
  pilU Vibrio cholerae strain A1552

38.643

96.857

0.374