Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LAB08_RS28540 Genome accession   NZ_AP017423
Coordinates   6371369..6372403 (-) Length   344 a.a.
NCBI ID   WP_096514408.1    Uniprot ID   -
Organism   Pseudomonas izuensis strain LAB-08     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6366369..6377403
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAB08_RS28510 (LAB08_R57030) ruvX 6366490..6366927 (+) 438 WP_096514414.1 Holliday junction resolvase RuvX -
  LAB08_RS28515 (LAB08_R57040) pyrR 6367022..6367525 (+) 504 WP_096514413.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  LAB08_RS28520 (LAB08_R57050) - 6367553..6368557 (+) 1005 WP_096514412.1 aspartate carbamoyltransferase catalytic subunit -
  LAB08_RS28525 (LAB08_R57060) - 6368554..6369825 (+) 1272 WP_096514411.1 dihydroorotase -
  LAB08_RS28530 (LAB08_R57070) - 6369937..6370368 (-) 432 WP_096514410.1 NINE protein -
  LAB08_RS28535 (LAB08_R57080) - 6370618..6371238 (+) 621 WP_096514409.1 C40 family peptidase -
  LAB08_RS28540 (LAB08_R57090) pilT 6371369..6372403 (-) 1035 WP_096514408.1 type IV pilus twitching motility protein PilT Machinery gene
  LAB08_RS28545 (LAB08_R57100) - 6372461..6373147 (+) 687 WP_096514407.1 YggS family pyridoxal phosphate-dependent enzyme -
  LAB08_RS28550 (LAB08_R57110) proC 6373182..6374000 (+) 819 WP_096514406.1 pyrroline-5-carboxylate reductase -
  LAB08_RS28555 (LAB08_R57120) - 6374025..6374612 (+) 588 WP_096514405.1 YggT family protein -
  LAB08_RS28560 (LAB08_R57130) - 6374805..6375944 (+) 1140 WP_096514404.1 homoserine O-acetyltransferase -
  LAB08_RS28565 (LAB08_R57140) metW 6375952..6376572 (+) 621 WP_060542415.1 methionine biosynthesis protein MetW -
  LAB08_RS28570 (LAB08_R57150) - 6376598..6377032 (+) 435 WP_096514403.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37950.35 Da        Isoelectric Point: 6.9824

>NTDB_id=67932 LAB08_RS28540 WP_096514408.1 6371369..6372403(-) (pilT) [Pseudomonas izuensis strain LAB-08]
MDITELLAFSARQGASDLHLSAGLPPMIRVDGDVRRINLPPLDHKEVQALIYDIMNDTQRVDFEKHLETDFSFDVPGVAR
FRGNAFNQSRGAGAVFRTIPSRVQSMDELGMGDVFRKMTDAPRGLVLVTGPTGSGKSTTLAAMIDYLNNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSSIQAGGSLGMQTLDMGL
RDLVARGLVSRDHAREKARSSDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=67932 LAB08_RS28540 WP_096514408.1 6371369..6372403(-) (pilT) [Pseudomonas izuensis strain LAB-08]
ATGGATATCACTGAGTTGTTGGCGTTCAGTGCCAGACAAGGAGCTTCGGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGCGTTGACGGTGATGTGCGGCGTATCAACTTGCCGCCCTTGGATCACAAGGAAGTACAGGCGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTGGATTTCGAAAAGCATCTGGAAACCGACTTTTCCTTCGACGTGCCCGGGGTGGCGCGT
TTCCGGGGCAACGCGTTCAATCAGAGCCGCGGAGCCGGCGCGGTGTTCCGCACGATCCCGTCCAGGGTCCAGAGCATGGA
CGAGCTGGGCATGGGCGACGTGTTCCGCAAGATGACCGATGCGCCCCGGGGGCTGGTGCTGGTGACCGGGCCGACCGGTT
CCGGCAAGTCCACCACGCTGGCGGCGATGATCGATTACCTGAACAATCATCGCCATCACCATATCCTCACCATCGAAGAT
CCGATCGAGTTCGTCCACGAGTCGCGTAAATGCCTGATCAATCAGCGCGAAGTCCACCGCGATACCCGCAGCTTCGCCAC
GGCCCTGCGCTCGGCGCTGCGCGAGGACCCGGATGTGATTCTGGTGGGGGAGATGCGCGATCTGGAAACGATTCGCCTGG
CGCTGACCGCTGCCGAAACCGGTCACCTGGTGTTCGGCACGCTGCACACCACGTCGGCGGCGAAGACCATCGACCGGATT
GTCGATGTGTTTCCGGGGGACGAGAAATCCATGGTGCGTTCGATGCTCTCCGAATCGCTGCTGGCGGTGGTGTCCCAGAC
GCTGGTCAAGAAAATCGGCGGCGGGCGTGTAGCCGCCCACGAAATCATGCTGGGTACGTCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCATCGATTCAAGCGGGCGGGTCGCTGGGGATGCAGACGCTGGATATGGGCTTG
AGGGATCTGGTGGCCCGGGGATTGGTCAGCCGTGATCATGCGCGGGAGAAGGCGCGGTCGTCGGATAATTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.012

100

0.84
  pilT Pseudomonas aeruginosa PAK

84.012

100

0.84
  pilT Acinetobacter baylyi ADP1

76.163

100

0.762
  pilT Acinetobacter baumannii D1279779

75.581

100

0.756
  pilT Acinetobacter baumannii strain A118

75.581

100

0.756
  pilT Acinetobacter nosocomialis M2

75.291

100

0.753
  pilT Legionella pneumophila strain Lp02

70.64

100

0.706
  pilT Legionella pneumophila strain ERS1305867

70.64

100

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.965

0.657
  pilT Vibrio cholerae strain A1552

67.062

97.965

0.657
  pilT Neisseria meningitidis 8013

64.928

100

0.651
  pilT Neisseria gonorrhoeae MS11

64.638

100

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment