Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MYA83_RS28410 Genome accession   NZ_CP095871
Coordinates   6255277..6256308 (-) Length   343 a.a.
NCBI ID   WP_419794265.1    Uniprot ID   -
Organism   Pseudomonas palleroniana strain B-BH16-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6250277..6261308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYA83_RS28380 (MYA83_28210) ruvX 6250381..6250818 (+) 438 WP_003195067.1 Holliday junction resolvase RuvX -
  MYA83_RS28385 (MYA83_28215) pyrR 6250891..6251397 (+) 507 WP_060753457.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MYA83_RS28390 (MYA83_28220) - 6251422..6252426 (+) 1005 WP_060753458.1 aspartate carbamoyltransferase catalytic subunit -
  MYA83_RS28395 (MYA83_28225) - 6252423..6253694 (+) 1272 WP_104994927.1 dihydroorotase -
  MYA83_RS28400 (MYA83_28230) - 6253826..6254254 (-) 429 WP_060753460.1 NINE protein -
  MYA83_RS28405 (MYA83_28235) - 6254504..6255139 (+) 636 WP_060753461.1 C40 family peptidase -
  MYA83_RS28410 (MYA83_28240) pilT 6255277..6256308 (-) 1032 WP_419794265.1 type IV pilus twitching motility protein PilT Machinery gene
  MYA83_RS28415 (MYA83_28245) - 6256366..6257052 (+) 687 WP_419794266.1 YggS family pyridoxal phosphate-dependent enzyme -
  MYA83_RS28420 (MYA83_28250) proC 6257086..6257904 (+) 819 WP_090368224.1 pyrroline-5-carboxylate reductase -
  MYA83_RS28425 (MYA83_28255) - 6257930..6258517 (+) 588 WP_060753465.1 YggT family protein -
  MYA83_RS28430 (MYA83_28260) metX 6258708..6259847 (+) 1140 WP_090368222.1 homoserine O-succinyltransferase MetX -
  MYA83_RS28435 (MYA83_28265) metW 6259855..6260475 (+) 621 WP_060753467.1 methionine biosynthesis protein MetW -
  MYA83_RS28440 (MYA83_28270) - 6260497..6260931 (+) 435 WP_090368220.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37095.77 Da        Isoelectric Point: 7.3738

>NTDB_id=678920 MYA83_RS28410 WP_419794265.1 6255277..6256308(-) (pilT) [Pseudomonas palleroniana strain B-BH16-1]
MDITQLLTASVRRGASDLHLSAGLAPMLRIDGEVWPLEGSVLSPPQVADLLSPLLNQHQQKDFETSLETDFAFELPGVAR
FRANVFRHDRGMGAVFRTIAQVRSLESLGLGEVFQRIAQLPRGLVLVTGPTGSGKSTTLAAMIDHLNQHRRQHILTLEDP
IEFIHKPKSALINQRQVHRDTHSFSAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTASAAKTVDRLV
DVFPAGEKAMVRSMLSESLQAVVSQALVKKIGGGRVAAHEIMLGTPAIRNLIREDKGAQLVSAIQTGGALGMNTLDMSLK
ALVSEGLVSREAARELARVPADM

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=678920 MYA83_RS28410 WP_419794265.1 6255277..6256308(-) (pilT) [Pseudomonas palleroniana strain B-BH16-1]
ATGGATATCACGCAATTGCTCACGGCCAGCGTGCGCCGTGGCGCCTCAGACCTGCATTTGTCCGCCGGTCTGGCGCCGAT
GCTGCGCATCGATGGCGAAGTCTGGCCCCTGGAGGGGTCGGTACTTTCGCCACCGCAAGTTGCGGACTTATTGAGCCCTT
TGCTCAATCAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCATTTGAATTGCCTGGCGTGGCGCGC
TTTCGGGCCAATGTGTTCCGACACGATCGCGGCATGGGGGCGGTGTTTCGTACTATTGCTCAGGTGCGTAGCCTGGAAAG
CCTGGGTCTCGGGGAGGTGTTCCAGCGTATCGCCCAATTGCCTCGTGGGCTGGTACTGGTGACCGGGCCGACCGGGTCGG
GCAAGTCCACGACACTGGCGGCGATGATCGACCACCTCAACCAGCATCGGCGCCAGCACATCCTCACCCTCGAAGACCCC
ATCGAATTTATCCACAAACCCAAATCGGCGTTGATCAACCAGCGGCAGGTGCACCGTGATACCCACAGCTTCTCGGCGGC
CCTGCGTTCGGCGTTGCGCGAAGACCCGGATGTGATTCTGGTGGGAGAGCTGCGCGACCTGGAAACCATCCGCCTCGCGC
TGACGGCGGCCGAGACCGGGCACCTGGTCCTGGGCACCCTGCACACCGCGTCCGCGGCGAAAACCGTGGACAGGCTGGTG
GATGTGTTCCCGGCGGGGGAAAAGGCCATGGTGCGTTCGATGCTATCGGAGTCTTTGCAGGCCGTGGTGTCCCAGGCGCT
GGTCAAGAAGATCGGCGGCGGGCGGGTAGCGGCTCACGAGATCATGCTGGGCACGCCGGCCATTCGTAACCTGATCCGCG
AGGACAAGGGTGCACAGCTGGTTTCAGCGATCCAGACCGGTGGGGCATTGGGGATGAATACGTTGGATATGAGCTTGAAG
GCGCTGGTGAGCGAGGGGCTGGTCAGCCGGGAGGCTGCGCGTGAGCTGGCGAGGGTTCCCGCAGACATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

70.93

100

0.711
  pilT Pseudomonas stutzeri DSM 10701

68.605

100

0.688
  pilT Acinetobacter nosocomialis M2

67.449

99.417

0.671
  pilT Acinetobacter baumannii D1279779

67.155

99.417

0.668
  pilT Acinetobacter baumannii strain A118

67.155

99.417

0.668
  pilT Acinetobacter baylyi ADP1

66.862

99.417

0.665
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.134

95.918

0.615
  pilT Vibrio cholerae strain A1552

64.134

95.918

0.615
  pilT Legionella pneumophila strain Lp02

61.834

98.542

0.609
  pilT Legionella pneumophila strain ERS1305867

61.834

98.542

0.609
  pilT Neisseria meningitidis 8013

60.29

100

0.606
  pilT Neisseria gonorrhoeae MS11

60.29

100

0.606
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.496
  pilU Vibrio cholerae strain A1552

39.701

97.668

0.388
  pilU Acinetobacter baylyi ADP1

38.15

100

0.385
  pilU Pseudomonas stutzeri DSM 10701

39.104

97.668

0.382