Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EL361_RS05410 Genome accession   NZ_AP017378
Coordinates   1168528..1169610 (-) Length   360 a.a.
NCBI ID   WP_126377380.1    Uniprot ID   A0A2Z6AXD1
Organism   Desulfovibrio ferrophilus strain IS5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1163528..1174610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL361_RS05380 (DFE_1074) - 1164209..1165018 (-) 810 WP_126377368.1 hypothetical protein -
  EL361_RS05385 (DFE_1075) - 1165162..1166067 (-) 906 WP_172961644.1 tetratricopeptide repeat protein -
  EL361_RS05390 (DFE_1076) - 1166051..1166389 (-) 339 WP_126377372.1 FtsB family cell division protein -
  EL361_RS05395 (DFE_1077) - 1166392..1166592 (-) 201 WP_338031062.1 hypothetical protein -
  EL361_RS05400 (DFE_1078) - 1166682..1167329 (-) 648 WP_126377376.1 hypothetical protein -
  EL361_RS05405 (DFE_1079) - 1167344..1168507 (-) 1164 WP_126377378.1 type IV pilus twitching motility protein PilT -
  EL361_RS05410 (DFE_1080) pilT 1168528..1169610 (-) 1083 WP_126377380.1 type IV pilus twitching motility protein PilT Machinery gene
  EL361_RS05415 (DFE_1081) pgsA 1169674..1170246 (-) 573 WP_126377383.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  EL361_RS05420 (DFE_1082) - 1170250..1170831 (-) 582 WP_232034880.1 lytic transglycosylase domain-containing protein -
  EL361_RS05425 (DFE_1083) - 1170869..1171777 (-) 909 WP_126377388.1 Mrp/NBP35 family ATP-binding protein -
  EL361_RS05430 (DFE_1084) - 1171931..1172899 (-) 969 WP_126377390.1 DUF368 domain-containing protein -
  EL361_RS05435 (DFE_1085) - 1172903..1173514 (-) 612 WP_126377392.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase -
  EL361_RS05440 (DFE_1086) - 1173718..1174155 (+) 438 WP_126377394.1 CBS domain-containing protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40370.69 Da        Isoelectric Point: 6.4023

>NTDB_id=67892 EL361_RS05410 WP_126377380.1 1168528..1169610(-) (pilT) [Desulfovibrio ferrophilus strain IS5]
MAQIDAFFKMMNEMGASDLHLSSGNQPIVRLHGDLERVKYKLLEHDELKKLLYEITPEAKIKEFEEKGDIDFAYEVPGLA
RYRVNFFNQARGCAAVFREIPSEILTVEQLSLPPLLKNLAMLPKGLVLVTGPTGSGKSTTLAAIIDYANKMRKQHVLTIE
DPIEFVHKPQQCLINQRELGRDTKSFGAAIRGALREDPDIILVGEMRDLETIQLAIEAAETGHLVFSTLHTISAAKTVDR
IIEVFPGDLQEQIRAGLSESLRAVVSQTLFKRIDKKGRCAALEILIGVPAVRNLIRENKTFQINSTMETGKKFGMQTLDD
EIARLLSLGWISPNEAYEKCLDKARFKQYLTKAPDDFTDA

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=67892 EL361_RS05410 WP_126377380.1 1168528..1169610(-) (pilT) [Desulfovibrio ferrophilus strain IS5]
ATGGCCCAGATTGATGCCTTCTTTAAGATGATGAACGAGATGGGTGCTTCCGACTTGCACCTTTCTTCCGGGAACCAACC
CATTGTCCGCCTGCATGGCGATTTGGAACGGGTGAAGTACAAACTCCTGGAGCACGACGAACTCAAGAAACTGCTCTACG
AGATTACCCCCGAAGCCAAGATCAAGGAGTTCGAGGAAAAGGGCGACATCGACTTCGCCTACGAAGTCCCTGGCCTGGCC
CGTTACAGAGTCAACTTTTTTAATCAGGCCCGCGGATGCGCAGCCGTTTTCCGTGAAATCCCTTCCGAAATCCTGACGGT
TGAGCAACTCAGTCTGCCACCGCTCCTTAAAAACCTTGCCATGCTGCCCAAGGGACTCGTGCTGGTCACCGGCCCTACCG
GCTCAGGTAAATCCACCACTCTCGCTGCAATTATCGATTATGCCAACAAGATGCGCAAGCAGCATGTGTTGACCATTGAA
GATCCCATTGAGTTTGTACACAAGCCGCAGCAGTGTCTGATCAACCAGCGCGAATTGGGGCGTGACACAAAGAGTTTTGG
CGCGGCCATCCGCGGCGCACTTCGCGAAGACCCGGATATCATCCTAGTCGGCGAAATGCGTGACCTGGAGACTATTCAGT
TGGCTATTGAAGCCGCTGAAACCGGTCACCTCGTGTTCTCGACTCTGCATACCATCTCGGCCGCCAAGACCGTTGACCGT
ATCATTGAGGTCTTTCCTGGAGACCTGCAGGAACAGATTCGTGCAGGGCTCTCTGAGTCATTGCGTGCTGTTGTTTCCCA
GACCTTGTTCAAGCGCATAGACAAGAAAGGCCGTTGTGCGGCCTTGGAAATTCTGATTGGTGTTCCGGCTGTCCGCAACC
TGATCCGTGAGAACAAGACCTTCCAGATCAACTCCACCATGGAGACCGGAAAGAAGTTTGGCATGCAGACCTTGGATGAT
GAGATTGCCAGGCTCTTGAGTCTGGGCTGGATCAGCCCCAACGAGGCCTATGAGAAGTGTCTCGATAAGGCGAGGTTCAA
GCAGTATTTGACCAAAGCCCCGGACGACTTTACCGACGCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-249)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2Z6AXD1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

59.317

89.444

0.531
  pilT Legionella pneumophila strain ERS1305867

57.704

91.944

0.531
  pilT Legionella pneumophila strain Lp02

57.704

91.944

0.531
  pilT Pseudomonas aeruginosa PAK

58.589

90.556

0.531
  pilT Acinetobacter baumannii strain A118

59.006

89.444

0.528
  pilT Acinetobacter baumannii D1279779

59.006

89.444

0.528
  pilT Pseudomonas stutzeri DSM 10701

58.282

90.556

0.528
  pilT Acinetobacter baylyi ADP1

58.991

88.056

0.519
  pilT Neisseria meningitidis 8013

55.692

90.278

0.503
  pilT Neisseria gonorrhoeae MS11

55.385

90.278

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

55.385

90.278

0.5
  pilT Vibrio cholerae strain A1552

55.385

90.278

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.714

97.222

0.464
  pilU Vibrio cholerae strain A1552

37.921

98.889

0.375
  pilU Pseudomonas stutzeri DSM 10701

39.82

92.778

0.369
  pilU Acinetobacter baylyi ADP1

39.577

91.944

0.364

Multiple sequence alignment