Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MYF53_RS09560 Genome accession   NZ_CP095843
Coordinates   1947307..1948287 (-) Length   326 a.a.
NCBI ID   WP_182245884.1    Uniprot ID   -
Organism   Escherichia fergusonii strain EF21QZZ116     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1942307..1953287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYF53_RS09530 (MYF53_09530) - 1943031..1943528 (+) 498 WP_002431380.1 SprT family zinc-dependent metalloprotease -
  MYF53_RS09535 (MYF53_09535) endA 1943623..1944330 (+) 708 WP_182245882.1 deoxyribonuclease I -
  MYF53_RS09540 (MYF53_09540) rsmE 1944410..1945141 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MYF53_RS09545 (MYF53_09545) gshB 1945154..1946104 (+) 951 WP_182245883.1 glutathione synthase -
  MYF53_RS09550 (MYF53_09550) - 1946213..1946776 (+) 564 WP_002431379.1 YqgE/AlgH family protein -
  MYF53_RS09555 (MYF53_09555) ruvX 1946776..1947192 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  MYF53_RS09560 (MYF53_09560) pilT 1947307..1948287 (-) 981 WP_182245884.1 type IV pilus twitching motility protein PilT Machinery gene
  MYF53_RS09565 (MYF53_09565) yggS 1948305..1949009 (+) 705 WP_002431377.1 pyridoxal phosphate homeostasis protein -
  MYF53_RS09570 (MYF53_09570) yggT 1949027..1949593 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  MYF53_RS09575 (MYF53_09575) yggU 1949590..1949880 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  MYF53_RS09580 (MYF53_09580) - 1949888..1950481 (+) 594 WP_001174747.1 XTP/dITP diphosphatase -
  MYF53_RS09585 (MYF53_09585) hemW 1950474..1951610 (+) 1137 WP_000239968.1 radical SAM family heme chaperone HemW -
  MYF53_RS09590 (MYF53_09590) - 1951924..1952910 (+) 987 WP_000783993.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36081.32 Da        Isoelectric Point: 5.8348

>NTDB_id=678792 MYF53_RS09560 WP_182245884.1 1947307..1948287(-) (pilT) [Escherichia fergusonii strain EF21QZZ116]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSYCPQLEQLGAPPILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQERRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=678792 MYF53_RS09560 WP_182245884.1 1947307..1948287(-) (pilT) [Escherichia fergusonii strain EF21QZZ116]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGGATGGAAGCTGCGCCGTTTGATGCGCCGGACGTGGAAGAGCTATTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGTTAGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCTCTGGCGTTACGGTTGTTACCTTCGTACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGAAGTGGAAAAT
CTACCACGCTGGCAGCGATGGTTGGTTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGGTGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGCATGACGTTCGCATCGGGATTGCG
TGCCGCATTGCGGGAAGATCCCGATGTGATATTGCTCGGAGAGTTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCACCTGGTGCTGGCAACTTTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCCGGGAGCTTGCGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAAGAAGAGTAGCGCTGTTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATACGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACTGGGCAGCAGGTGGGGATGTTGACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

37.462

100

0.38
  pilU Acinetobacter baylyi ADP1

37.578

98.773

0.371