Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MMY93_RS04250 Genome accession   NZ_CP095761
Coordinates   871207..872187 (+) Length   326 a.a.
NCBI ID   WP_004854153.1    Uniprot ID   A0A2J4RH58
Organism   Klebsiella michiganensis strain K254     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 866207..877187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMY93_RS04220 (MMY93_04220) - 866589..867575 (-) 987 WP_004854161.1 TRAP transporter substrate-binding protein -
  MMY93_RS04225 (MMY93_04225) hemW 867880..869016 (-) 1137 WP_197663955.1 radical SAM family heme chaperone HemW -
  MMY93_RS04230 (MMY93_04230) - 869009..869602 (-) 594 WP_004854157.1 XTP/dITP diphosphatase -
  MMY93_RS04235 (MMY93_04235) yggU 869615..869905 (-) 291 WP_004854155.1 DUF167 family protein YggU -
  MMY93_RS04240 (MMY93_04240) - 869902..870468 (-) 567 WP_004105714.1 YggT family protein -
  MMY93_RS04245 (MMY93_04245) - 870486..871190 (-) 705 WP_009651823.1 YggS family pyridoxal phosphate-dependent enzyme -
  MMY93_RS04250 (MMY93_04250) pilT 871207..872187 (+) 981 WP_004854153.1 type IV pilus twitching motility protein PilT Machinery gene
  MMY93_RS04255 (MMY93_04255) - 872536..873189 (+) 654 WP_004854152.1 LuxR C-terminal-related transcriptional regulator -
  MMY93_RS04260 (MMY93_04260) ruvX 873245..873661 (-) 417 WP_181250250.1 Holliday junction resolvase RuvX -
  MMY93_RS04265 (MMY93_04265) - 873661..874224 (-) 564 WP_197663957.1 YqgE/AlgH family protein -
  MMY93_RS04270 (MMY93_04270) gshB 874401..875348 (-) 948 WP_004105704.1 glutathione synthase -
  MMY93_RS04275 (MMY93_04275) rsmE 875369..876100 (-) 732 WP_004854148.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MMY93_RS04280 (MMY93_04280) endA 876192..876899 (-) 708 WP_197663959.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35242.42 Da        Isoelectric Point: 6.7582

>NTDB_id=678211 MMY93_RS04250 WP_004854153.1 871207..872187(+) (pilT) [Klebsiella michiganensis strain K254]
MELEEIVTLSVKHNVSDLHLCNASAPRWRRQGKLEPAPFTSPDIGKILADWLNAEQLAQWQSCGQVDFALAPAGGPRLRA
SAFVHNRGLSLALRLLAETCPQLADLGAPAALTELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLNGHILTLEDPVE
FIHRSERCLIQQREIGRHCPSFASALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRSQLAGSLCAVLAQKLFPDTAGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQFGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=678211 MMY93_RS04250 WP_004854153.1 871207..872187(+) (pilT) [Klebsiella michiganensis strain K254]
ATGGAACTGGAAGAAATAGTGACCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCGTCAGCACCGCG
CTGGCGTCGCCAGGGCAAGCTGGAGCCTGCGCCTTTTACCTCTCCGGATATTGGCAAGATACTCGCTGATTGGCTCAACG
CCGAGCAGCTCGCCCAGTGGCAGTCCTGCGGACAGGTCGATTTCGCGCTGGCTCCGGCCGGCGGCCCGCGCCTGCGCGCC
AGCGCATTTGTTCACAACCGCGGTCTGTCGCTGGCCCTGCGTCTGCTGGCGGAAACCTGCCCGCAGCTGGCGGATCTCGG
CGCACCTGCCGCCTTAACGGAGCTGCTCAATGAGGAGAGCGGGCTTATCCTGGTCACGGGCGCCACCGGTAGCGGGAAAT
CCACCACCCTGGCGGCGATGGTGGGCTATCTTAATCAGCGCCTCAATGGCCATATCCTGACGCTGGAAGATCCGGTTGAG
TTTATTCATCGCAGCGAGCGCTGCCTGATCCAGCAGCGGGAAATCGGCCGCCACTGTCCCTCATTTGCCTCGGCCCTGCG
CGCTGCGCTGCGTCAGGACCCGGATGTGATTCTGTTAGGGGAACTGCGTGACAGCGAAACGATCCGCCTGGCATTGACCG
CGGCGGAGACCGGACATCTGGTTATGGCGACGCTGCATACGCGCGGCGCGGCACAGGCGGTTGAAAGGCTGGTGGATGTC
TTTCCTGCCCAGGAGAAAGAGCAGGTTCGTAGCCAGCTGGCGGGAAGTTTATGCGCGGTGCTGGCACAAAAATTGTTTCC
GGATACTGCTGGCGGAAGAGTTGCGCTATACGAGCTTTTGGTTAATACCCCGGCGGTGGCGAATTTGATCCGGGAGGGGA
AAACGCATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAATTCGGGATGCAAACTTTTACGCAAAGCTTTCAGCAACGT
ACGGCTGCGGGTCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2J4RH58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.848

100

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.848

100

0.503
  pilT Neisseria meningitidis 8013

47.879

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.576

100

0.482
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Acinetobacter baumannii D1279779

45.152

100

0.457
  pilT Acinetobacter baumannii strain A118

45.152

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter nosocomialis M2

44.848

100

0.454
  pilT Acinetobacter baylyi ADP1

44.681

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.414
  pilU Vibrio cholerae strain A1552

40.683

98.773

0.402
  pilU Acinetobacter baylyi ADP1

38.39

99.08

0.38