Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L2Y97_RS04240 Genome accession   NZ_CP095742
Coordinates   958306..959415 (-) Length   369 a.a.
NCBI ID   WP_283248300.1    Uniprot ID   -
Organism   Luteibacter aegosomatissinici strain 353     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 953306..964415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y97_RS04220 (L2Y97_04220) - 953445..955550 (+) 2106 WP_247433411.1 acyltransferase family protein -
  L2Y97_RS04225 (L2Y97_04225) - 955640..956272 (-) 633 WP_247433414.1 HAD hydrolase-like protein -
  L2Y97_RS04230 (L2Y97_04230) - 956272..957453 (-) 1182 WP_247433417.1 phosphoglycerate kinase -
  L2Y97_RS04235 (L2Y97_04235) maiA 957535..958185 (+) 651 WP_247433420.1 maleylacetoacetate isomerase -
  L2Y97_RS04240 (L2Y97_04240) pilU 958306..959415 (-) 1110 WP_283248300.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Y97_RS04245 (L2Y97_04245) - 959509..960084 (+) 576 WP_247433423.1 DUF4398 domain-containing protein -
  L2Y97_RS04250 (L2Y97_04250) - 960084..960746 (+) 663 WP_247433426.1 DUF4398 domain-containing protein -
  L2Y97_RS04255 (L2Y97_04255) - 961145..961375 (+) 231 WP_247433427.1 YdcH family protein -
  L2Y97_RS04260 (L2Y97_04260) - 961586..962956 (+) 1371 WP_247433429.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41092.35 Da        Isoelectric Point: 6.5085

>NTDB_id=677993 L2Y97_RS04240 WP_283248300.1 958306..959415(-) (pilU) [Luteibacter aegosomatissinici strain 353]
MDIGYFLKLMVDKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPSGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEIGMVIRAIKSEIPSLEELQLPGIFRELIMEPRGLILVVGATGSGKSTTLAAMLDHRNTNSSGHILTIED
PIEYLHRHKRSIVNQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTETMEAAISFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRSVISQRLVLGKDGRRMPATEVLLNTPLIRDMIRRGQIHEVKDAMDRSLQEGMQTFDQSL
YRLYKEGKITLEECLNKADSRDGLALKIRLSEGATGNEFASSDPYGIGV

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=677993 L2Y97_RS04240 WP_283248300.1 958306..959415(-) (pilU) [Luteibacter aegosomatissinici strain 353]
GTGGATATCGGTTATTTCCTGAAACTGATGGTGGACAAGGGCGCGTCGGACATGTTCCTGACGACCGGTGCCCCCGTGAA
CATCAAGGTGGAAGGCAAGCTTTACCCGCTTGGTAACACGGGCCTGCCCAGCGGCATGGTCAAGAAGATCGCCTATTCCC
TCATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGACCTTGAGCTGAATATGGCCCTGGCCGTGAAGGAAGCCGGCCGC
TTCCGTATTAACGTTTTCAAGCAGCGCGGCGAGATCGGCATGGTCATCCGTGCGATCAAGAGCGAGATCCCCTCGCTGGA
AGAGCTGCAGCTGCCGGGCATTTTCCGCGAGCTGATCATGGAGCCGCGCGGCCTCATCCTGGTCGTCGGCGCCACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGCTCGACCACCGCAACACCAATAGCTCGGGCCATATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCACAAACGTTCCATCGTGAACCAGCGTGAGGTGGGCCTGGACACCCACAGCTATCACGA
GGCGCTGAAGAACGCGATGCGCGAGGCGCCGGATGTGATCATGATCGGCGAAATTCGCGACACGGAGACCATGGAAGCGG
CCATTTCCTTCTCGGAAACCGGCCACCTCTGCCTGGCCACCCTGCACTCGAACAATGCCGACCAGACCCTTGAGCGCATC
CTGAACTTCTTCCCGGAATCGGCACACAAGAACGTGCTGATGAACCTGGCCCTGAACCTGCGCTCGGTGATCAGCCAGCG
TCTGGTGCTGGGCAAGGATGGCCGCCGCATGCCGGCCACCGAAGTACTGCTCAACACCCCGCTGATCCGCGACATGATCC
GCCGCGGCCAGATCCACGAGGTGAAGGACGCCATGGACCGCAGCCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGCTC
TATCGCCTGTACAAGGAAGGCAAGATCACGCTCGAGGAGTGCCTTAACAAGGCCGATTCGCGTGATGGCCTGGCGCTGAA
GATCCGCCTGTCGGAAGGCGCGACGGGCAATGAGTTTGCCTCGAGCGATCCGTACGGCATCGGCGTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.829

98.103

0.499
  pilU Vibrio cholerae strain A1552

49.593

100

0.496
  pilU Acinetobacter baylyi ADP1

49.719

96.477

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.896

99.187

0.425
  pilT Pseudomonas aeruginosa PAK

42.012

91.599

0.385
  pilT Acinetobacter baylyi ADP1

42.598

89.702

0.382
  pilT Legionella pneumophila strain Lp02

40.936

92.683

0.379
  pilT Legionella pneumophila strain ERS1305867

40.936

92.683

0.379
  pilT Acinetobacter baumannii strain A118

41.818

89.431

0.374
  pilT Acinetobacter nosocomialis M2

41.818

89.431

0.374
  pilT Acinetobacter baumannii D1279779

41.818

89.431

0.374
  pilT Pseudomonas stutzeri DSM 10701

40.828

91.599

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.763

91.328

0.363
  pilT Vibrio cholerae strain A1552

39.763

91.328

0.363
  pilT Neisseria gonorrhoeae MS11

38.329

94.038

0.36
  pilT Neisseria meningitidis 8013

38.329

94.038

0.36