Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L2Y96_RS04745 Genome accession   NZ_CP095741
Coordinates   1071735..1072844 (-) Length   369 a.a.
NCBI ID   WP_283248862.1    Uniprot ID   -
Organism   Luteibacter aegosomaticola strain 335     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1066735..1077844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y96_RS04725 (L2Y96_04725) - 1068558..1069046 (-) 489 WP_247333095.1 hypothetical protein -
  L2Y96_RS04730 (L2Y96_04730) - 1069097..1069729 (-) 633 WP_247333097.1 HAD hydrolase-like protein -
  L2Y96_RS04735 (L2Y96_04735) - 1069729..1070910 (-) 1182 WP_247333099.1 phosphoglycerate kinase -
  L2Y96_RS04740 (L2Y96_04740) maiA 1070993..1071646 (+) 654 WP_247333101.1 maleylacetoacetate isomerase -
  L2Y96_RS04745 (L2Y96_04745) pilU 1071735..1072844 (-) 1110 WP_283248862.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Y96_RS04750 (L2Y96_04750) - 1072938..1073513 (+) 576 WP_247333103.1 DUF4398 domain-containing protein -
  L2Y96_RS04755 (L2Y96_04755) - 1073513..1074175 (+) 663 WP_247333105.1 DUF4398 domain-containing protein -
  L2Y96_RS04760 (L2Y96_04760) - 1074553..1074783 (+) 231 WP_247333107.1 YdcH family protein -
  L2Y96_RS04765 (L2Y96_04765) - 1074989..1076359 (+) 1371 WP_247333109.1 pyridoxal-phosphate dependent enzyme -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41090.34 Da        Isoelectric Point: 6.5082

>NTDB_id=677973 L2Y96_RS04745 WP_283248862.1 1071735..1072844(-) (pilU) [Luteibacter aegosomaticola strain 335]
MDIGYFLKLMVDKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPSGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEIGMVIRAIKSEIPSLEELQLPGIFRDLIMEPRGLILVVGATGSGKSTTLAAMLDHRNTNSSGHILTIED
PIEYLHRHKRSIVNQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTETMEAAIAFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRSVISQRLVLGKDGRRMPATEVLLNTPLIRDMIRRGQIHEVKDAMDRSLQEGMQTFDQSL
YRLYKEGRITLEECLNKADSRDGLALKIRLSEGATGNEFASSDPYGIGV

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=677973 L2Y96_RS04745 WP_283248862.1 1071735..1072844(-) (pilU) [Luteibacter aegosomaticola strain 335]
GTGGATATCGGTTATTTCCTGAAACTGATGGTGGACAAGGGCGCGTCGGACATGTTCCTGACGACCGGTGCCCCTGTGAA
CATCAAGGTGGAAGGCAAGCTTTACCCGCTTGGTAACACGGGCCTGCCCAGCGGCATGGTCAAGAAGATCGCCTATTCCC
TGATGGACGAGGGCCAGGTTCCCCAGTTCGAGCGCGATCTCGAGCTGAACATGGCGCTGGCGGTGAAGGAAGCCGGCCGT
TTCCGTATCAACGTTTTCAAGCAGCGTGGCGAGATCGGCATGGTTATCCGTGCCATCAAGAGCGAGATCCCGTCCCTGGA
AGAGCTCCAGTTGCCGGGTATTTTCCGCGACCTGATCATGGAGCCGCGCGGCCTTATCCTGGTCGTCGGCGCCACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGCTCGACCACCGCAATACCAATAGCTCGGGCCATATCCTCACGATCGAGGAC
CCCATCGAGTACCTGCACCGCCACAAGCGCTCCATCGTGAACCAGCGCGAAGTGGGCCTGGATACGCACAGCTATCACGA
GGCGTTGAAGAACGCGATGCGTGAAGCGCCCGATGTGATCATGATCGGCGAAATTCGCGACACGGAGACGATGGAAGCCG
CCATCGCCTTCTCGGAAACCGGCCACCTGTGCCTGGCGACCCTGCACTCTAACAATGCGGACCAGACCCTCGAGCGCATC
CTGAACTTCTTCCCGGAATCGGCACACAAGAACGTGCTGATGAACCTGGCCCTGAACCTGCGCTCGGTGATCAGCCAGCG
CCTGGTGCTGGGCAAGGACGGCCGCCGCATGCCGGCGACCGAAGTGCTGCTGAACACCCCGCTCATCCGCGACATGATCC
GCCGTGGCCAGATCCACGAGGTGAAGGACGCCATGGATCGCAGCCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGCTC
TACCGCCTGTACAAGGAAGGCCGGATCACGCTCGAGGAATGCCTGAACAAAGCAGATTCGCGTGATGGCCTGGCCCTGAA
GATCCGCCTGTCCGAGGGCGCGACCGGTAACGAGTTCGCTTCGAGCGATCCGTACGGGATCGGCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.105

98.103

0.501
  pilU Vibrio cholerae strain A1552

49.322

100

0.493
  pilU Acinetobacter baylyi ADP1

49.719

96.477

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.169

99.187

0.428
  pilT Pseudomonas aeruginosa PAK

42.012

91.599

0.385
  pilT Acinetobacter baylyi ADP1

42.9

89.702

0.385
  pilT Acinetobacter baumannii D1279779

42.121

89.431

0.377
  pilT Acinetobacter nosocomialis M2

42.121

89.431

0.377
  pilT Acinetobacter baumannii strain A118

42.121

89.431

0.377
  pilT Legionella pneumophila strain Lp02

40.643

92.683

0.377
  pilT Legionella pneumophila strain ERS1305867

40.643

92.683

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.828

91.599

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.763

91.328

0.363
  pilT Vibrio cholerae strain A1552

39.763

91.328

0.363
  pilT Neisseria gonorrhoeae MS11

38.329

94.038

0.36
  pilT Neisseria meningitidis 8013

38.329

94.038

0.36