Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   L2Y94_RS15985 Genome accession   NZ_CP095740
Coordinates   3555384..3555800 (+) Length   138 a.a.
NCBI ID   WP_247368866.1    Uniprot ID   -
Organism   Luteibacter aegosomatis strain 321     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3550877..3569888 3555384..3555800 within 0


Gene organization within MGE regions


Location: 3550877..3569888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y94_RS15975 (L2Y94_15975) - 3550877..3552802 (+) 1926 WP_247368831.1 hypothetical protein -
  L2Y94_RS15980 (L2Y94_15980) - 3552885..3554966 (-) 2082 WP_247368863.1 S9 family peptidase -
  L2Y94_RS15985 (L2Y94_15985) pilA2 3555384..3555800 (+) 417 WP_247368866.1 pilin Machinery gene
  L2Y94_RS15990 (L2Y94_15990) - 3555873..3557819 (+) 1947 WP_247368869.1 hypothetical protein -
  L2Y94_RS16000 (L2Y94_16000) - 3557939..3558379 (+) 441 WP_283248495.1 pilin -
  L2Y94_RS16005 (L2Y94_16005) - 3558547..3560454 (+) 1908 WP_247368871.1 hypothetical protein -
  L2Y94_RS16010 (L2Y94_16010) - 3560454..3561701 (+) 1248 WP_247368889.1 oligosaccharide flippase family protein -
  L2Y94_RS16015 (L2Y94_16015) - 3561657..3562547 (-) 891 WP_247368892.1 glycosyltransferase family 2 protein -
  L2Y94_RS16020 (L2Y94_16020) - 3562544..3563347 (-) 804 WP_247368894.1 class I SAM-dependent methyltransferase -
  L2Y94_RS16025 (L2Y94_16025) - 3563572..3564564 (-) 993 WP_247368897.1 glycosyltransferase family 4 protein -
  L2Y94_RS16030 (L2Y94_16030) - 3564591..3565748 (-) 1158 WP_247368900.1 glycosyltransferase family 4 protein -
  L2Y94_RS16035 (L2Y94_16035) - 3565759..3566481 (-) 723 WP_247368911.1 class I SAM-dependent methyltransferase -
  L2Y94_RS16040 (L2Y94_16040) - 3566478..3567350 (-) 873 WP_247368914.1 glycosyltransferase family 2 protein -
  L2Y94_RS16045 (L2Y94_16045) - 3567347..3568516 (-) 1170 WP_247368916.1 hypothetical protein -
  L2Y94_RS16050 (L2Y94_16050) - 3568491..3569888 (-) 1398 WP_247375289.1 MBOAT family O-acyltransferase -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14406.82 Da        Isoelectric Point: 9.7088

>NTDB_id=677963 L2Y94_RS15985 WP_247368866.1 3555384..3555800(+) (pilA2) [Luteibacter aegosomatis strain 321]
MKTIQKGFTLIELMIVVAIIAILAAIAIPQYQNYVKRAKVSEGLVLADAAKLAVAETFQSKGTMPTSASDAGYTATTSTY
VTSIGITGGTGVITVVYKNIGTGVDTKNIVLTPTTTTGVITWKCTGSLNAQFLPANCR

Nucleotide


Download         Length: 417 bp        

>NTDB_id=677963 L2Y94_RS15985 WP_247368866.1 3555384..3555800(+) (pilA2) [Luteibacter aegosomatis strain 321]
ATGAAGACCATCCAGAAGGGTTTCACGCTCATCGAACTGATGATCGTCGTCGCGATCATCGCCATCCTGGCCGCCATCGC
CATTCCGCAATACCAGAACTACGTAAAGCGCGCCAAGGTGTCGGAAGGCCTCGTGCTCGCCGATGCGGCCAAGCTAGCCG
TGGCGGAGACGTTCCAGTCCAAGGGCACGATGCCCACGAGCGCCTCGGATGCGGGCTACACCGCAACGACCAGCACGTAC
GTCACCTCCATCGGCATTACCGGCGGCACCGGTGTCATTACCGTCGTTTACAAGAACATCGGCACGGGCGTGGATACGAA
GAACATCGTTCTTACGCCGACCACCACCACTGGCGTGATCACCTGGAAGTGCACTGGCAGCCTGAATGCACAGTTCCTCC
CGGCGAATTGCCGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-27)

Pilin (bacterial filament) (Pilin)

(37-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

54.745

99.275

0.543
  pilA2 Legionella pneumophila strain ERS1305867

54.015

99.275

0.536
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.17

100

0.493
  pilA Ralstonia pseudosolanacearum GMI1000

40.244

100

0.478
  pilA Haemophilus influenzae Rd KW20

43.357

100

0.449
  pilA Acinetobacter baumannii strain A118

44.203

100

0.442
  pilA Haemophilus influenzae 86-028NP

42.657

100

0.442
  comP Acinetobacter baylyi ADP1

41.096

100

0.435
  pilA Pseudomonas aeruginosa PAK

39.865

100

0.428
  pilA/pilA1 Eikenella corrodens VA1

35.185

100

0.413
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.298

100

0.391
  pilA Vibrio campbellii strain DS40M4

38.849

100

0.391
  pilE Neisseria gonorrhoeae MS11

33.962

100

0.391
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.956

99.275

0.377
  pilE Neisseria gonorrhoeae strain FA1090

32.911

100

0.377
  pilA Glaesserella parasuis strain SC1401

35.616

100

0.377