Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L2Y94_RS03920 Genome accession   NZ_CP095740
Coordinates   885241..886350 (-) Length   369 a.a.
NCBI ID   WP_247373250.1    Uniprot ID   -
Organism   Luteibacter aegosomatis strain 321     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 879964..917944 885241..886350 within 0


Gene organization within MGE regions


Location: 879964..917944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Y94_RS03895 (L2Y94_03895) - 879964..882009 (+) 2046 WP_247373244.1 acyltransferase family protein -
  L2Y94_RS03900 (L2Y94_03900) - 882014..882328 (-) 315 WP_247373245.1 hypothetical protein -
  L2Y94_RS03905 (L2Y94_03905) - 882433..883068 (-) 636 WP_247373246.1 HAD hydrolase-like protein -
  L2Y94_RS03910 (L2Y94_03910) - 883068..884249 (-) 1182 WP_247373247.1 phosphoglycerate kinase -
  L2Y94_RS03915 (L2Y94_03915) maiA 884484..885128 (+) 645 WP_247373248.1 maleylacetoacetate isomerase -
  L2Y94_RS03920 (L2Y94_03920) pilU 885241..886350 (-) 1110 WP_247373250.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  L2Y94_RS03925 (L2Y94_03925) - 886443..886988 (+) 546 WP_247373252.1 DUF4398 domain-containing protein -
  L2Y94_RS03930 (L2Y94_03930) - 886988..887656 (+) 669 WP_247373253.1 DUF4398 domain-containing protein -
  L2Y94_RS03935 (L2Y94_03935) - 887962..888198 (+) 237 WP_345780013.1 YdcH family protein -
  L2Y94_RS03940 (L2Y94_03940) - 888401..889771 (+) 1371 WP_247373255.1 pyridoxal-phosphate dependent enzyme -
  L2Y94_RS03945 (L2Y94_03945) glmS 889787..891616 (+) 1830 WP_247373257.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -
  L2Y94_RS21300 - 891640..892071 (+) 432 Protein_782 acyltransferase -
  L2Y94_RS03960 (L2Y94_03960) - 892150..893493 (+) 1344 WP_247373258.1 phosphomannomutase -
  L2Y94_RS03965 (L2Y94_03965) - 893543..894967 (-) 1425 WP_247375112.1 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase -
  L2Y94_RS03970 (L2Y94_03970) rfbD 894973..895905 (-) 933 WP_247373259.1 dTDP-4-dehydrorhamnose reductase -
  L2Y94_RS03975 (L2Y94_03975) rfbC 895902..896459 (-) 558 WP_247373260.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  L2Y94_RS03980 (L2Y94_03980) rfbA 896456..897340 (-) 885 WP_247373261.1 glucose-1-phosphate thymidylyltransferase RfbA -
  L2Y94_RS03985 (L2Y94_03985) rfbB 897337..898386 (-) 1050 WP_247373262.1 dTDP-glucose 4,6-dehydratase -
  L2Y94_RS03990 (L2Y94_03990) - 898580..899758 (+) 1179 WP_256452174.1 PLP-dependent aspartate aminotransferase family protein -
  L2Y94_RS03995 (L2Y94_03995) - 899755..900600 (+) 846 WP_247373263.1 ABC transporter permease -
  L2Y94_RS04000 (L2Y94_04000) - 900590..901939 (+) 1350 WP_247373264.1 ABC transporter ATP-binding protein -
  L2Y94_RS04005 (L2Y94_04005) - 901936..906630 (+) 4695 WP_247373265.1 glycosyltransferase -
  L2Y94_RS04010 (L2Y94_04010) - 906627..907148 (+) 522 WP_247373266.1 hypothetical protein -
  L2Y94_RS04015 (L2Y94_04015) - 907170..908225 (+) 1056 WP_247373267.1 acyltransferase -
  L2Y94_RS04020 (L2Y94_04020) - 908545..910080 (-) 1536 WP_247373268.1 hypothetical protein -
  L2Y94_RS04025 (L2Y94_04025) - 910092..910517 (-) 426 WP_247373269.1 GtrA family protein -
  L2Y94_RS04030 (L2Y94_04030) - 910514..911248 (-) 735 WP_247373270.1 SDR family oxidoreductase -
  L2Y94_RS04035 (L2Y94_04035) - 911251..912552 (-) 1302 WP_247373272.1 FAD-binding oxidoreductase -
  L2Y94_RS04040 (L2Y94_04040) - 912552..913973 (-) 1422 WP_247373273.1 UbiA family prenyltransferase -
  L2Y94_RS04045 (L2Y94_04045) - 914088..915029 (-) 942 WP_247373274.1 electron transfer flavoprotein subunit alpha/FixB family protein -
  L2Y94_RS04050 (L2Y94_04050) - 915052..915798 (-) 747 WP_247373275.1 electron transfer flavoprotein subunit beta/FixA family protein -
  L2Y94_RS04055 (L2Y94_04055) - 916139..917944 (+) 1806 WP_247373276.1 LTA synthase family protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41045.20 Da        Isoelectric Point: 6.5082

>NTDB_id=677953 L2Y94_RS03920 WP_247373250.1 885241..886350(-) (pilU) [Luteibacter aegosomatis strain 321]
MDIGYFLKLMVDKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPSGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEIGMVIRAIKSEIPSLEELRQPAIFRELVMEPRGLILVVGATGSGKSTTLAAMLDHRNQNTSGHILTIED
PIEYLHRHKRSIVNQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTETMEAAIAFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVIGKDGRRVPATEVLLNTPLIRDMIRRGQIHEVKDAMDRSLQEGMQTFDQSL
YRLYKDGQITLEECLSKADSRDGLALKIRLSEGSSGNEFASSDPYGIGA

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=677953 L2Y94_RS03920 WP_247373250.1 885241..886350(-) (pilU) [Luteibacter aegosomatis strain 321]
GTGGATATTGGTTATTTCCTGAAGCTCATGGTGGACAAGGGTGCGTCGGACATGTTCCTGACGACCGGAGCCCCCGTGAA
CATCAAGGTGGAAGGCAAGCTCTACCCGTTGGGCAATACCGGCCTGCCCAGCGGCATGGTCAAGAAGATCGCCTACTCCC
TGATGGACGAAGGCCAGGTCCCCCAGTTCGAGCGCGACCTGGAACTGAACATGGCCCTGGCCGTGAAGGAGGCCGGGCGG
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAGATCGGCATGGTCATCCGTGCCATCAAGAGCGAGATCCCTTCGCTGGA
GGAATTGCGCCAGCCGGCCATCTTCCGCGAACTGGTCATGGAACCGCGCGGGCTGATCCTGGTGGTGGGCGCCACCGGTT
CGGGCAAGTCGACCACCCTGGCCGCCATGCTCGACCATCGCAACCAGAACACCTCGGGACACATCCTCACCATCGAGGAC
CCCATCGAATACCTGCACCGGCACAAGCGCTCCATCGTGAACCAGCGTGAGGTGGGCCTGGATACCCACAGTTACCACGA
GGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTCATCATGATCGGCGAGATCCGAGACACCGAGACCATGGAGGCGG
CCATCGCCTTCTCGGAAACCGGCCACCTCTGCCTGGCCACGCTCCATTCGAACAACGCCGACCAGACCCTCGAGCGCATC
CTCAACTTCTTCCCCGAGTCGGCCCACAAGAACGTGCTGATGAACCTCGCCCTGAACCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGATCGGCAAGGACGGCCGTCGCGTGCCGGCCACCGAGGTGCTGCTCAACACCCCGCTGATTCGCGACATGATCC
GCCGCGGCCAGATCCACGAGGTGAAGGACGCCATGGACCGCAGCCTCCAGGAAGGCATGCAGACCTTCGACCAGTCGCTC
TACCGGCTCTACAAGGACGGCCAGATCACCCTCGAGGAGTGCCTGTCCAAGGCCGATTCGCGCGATGGGCTGGCGCTCAA
GATCCGCCTGTCGGAAGGTTCGTCGGGGAACGAGTTCGCGTCAAGCGATCCGTACGGGATCGGGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.829

98.103

0.499
  pilU Vibrio cholerae strain A1552

49.459

100

0.496
  pilU Acinetobacter baylyi ADP1

50

95.935

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.896

99.187

0.425
  pilT Acinetobacter baylyi ADP1

42.353

92.141

0.39
  pilT Pseudomonas aeruginosa PAK

42.012

91.599

0.385
  pilT Acinetobacter baumannii D1279779

41.42

91.599

0.379
  pilT Acinetobacter nosocomialis M2

41.42

91.599

0.379
  pilT Pseudomonas stutzeri DSM 10701

41.42

91.599

0.379
  pilT Acinetobacter baumannii strain A118

41.42

91.599

0.379
  pilT Vibrio cholerae strain A1552

40.653

91.328

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.653

91.328

0.371
  pilT Legionella pneumophila strain Lp02

41.515

89.431

0.371
  pilT Legionella pneumophila strain ERS1305867

41.515

89.431

0.371
  pilT Neisseria gonorrhoeae MS11

38.905

94.038

0.366
  pilT Neisseria meningitidis 8013

38.905

94.038

0.366