Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MW397_RS18815 Genome accession   NZ_CP095529
Coordinates   3957068..3958048 (-) Length   326 a.a.
NCBI ID   WP_072005819.1    Uniprot ID   A0A8S7Y6M1
Organism   Escherichia coli strain GN06156     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3952068..3963048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW397_RS18785 (MW397_18775) yggI 3952792..3953289 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  MW397_RS18790 (MW397_18780) endA 3953384..3954091 (+) 708 WP_000286500.1 deoxyribonuclease I -
  MW397_RS18795 (MW397_18785) rsmE 3954171..3954902 (+) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MW397_RS18800 (MW397_18790) gshB 3954915..3955865 (+) 951 WP_000593273.1 glutathione synthase -
  MW397_RS18805 (MW397_18795) yqgE 3955974..3956537 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  MW397_RS18810 (MW397_18800) ruvX 3956537..3956953 (+) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  MW397_RS18815 (MW397_18805) pilT 3957068..3958048 (-) 981 WP_072005819.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MW397_RS18820 (MW397_18810) yggS 3958066..3958770 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  MW397_RS18825 (MW397_18815) yggT 3958788..3959354 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  MW397_RS18830 (MW397_18820) yggU 3959351..3959641 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  MW397_RS18835 (MW397_18825) rdgB 3959649..3960242 (+) 594 WP_001174738.1 XTP/dITP diphosphatase -
  MW397_RS18840 (MW397_18830) hemW 3960235..3961371 (+) 1137 WP_000239965.1 radical SAM family heme chaperone HemW -
  MW397_RS18845 (MW397_18835) - 3961688..3962674 (+) 987 WP_021513215.1 TRAP transporter substrate-binding protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36033.21 Da        Isoelectric Point: 5.7980

>NTDB_id=677385 MW397_RS18815 WP_072005819.1 3957068..3958048(-) (pilT) [Escherichia coli strain GN06156]
MYMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPILPELLKSENGLILVTGATGSGKSTTLAALVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGEVRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=677385 MW397_RS18815 WP_072005819.1 3957068..3958048(-) (pilT) [Escherichia coli strain GN06156]
ATGTATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCATTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGCATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
CGCGCCACCGATATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGTAAAT
CCACCACGCTGGCGGCATTGGTTGGCTATCTTAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGGTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGAGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACACGCCTGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.089

96.933

0.408
  pilU Vibrio cholerae strain A1552

39.748

97.239

0.387
  pilU Pseudomonas stutzeri DSM 10701

38.125

98.16

0.374
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365