Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MW395_RS25425 Genome accession   NZ_CP095526
Coordinates   5045862..5046842 (+) Length   326 a.a.
NCBI ID   WP_001309723.1    Uniprot ID   A0A148HZL8
Organism   Escherichia coli strain GN05505     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5040862..5051842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW395_RS25395 (MW395_25390) - 5041240..5042226 (-) 987 WP_000784004.1 TRAP transporter substrate-binding protein -
  MW395_RS25400 (MW395_25395) hemW 5042539..5043675 (-) 1137 WP_000239946.1 radical SAM family heme chaperone HemW -
  MW395_RS25405 (MW395_25400) rdgB 5043668..5044261 (-) 594 WP_001174736.1 XTP/dITP diphosphatase -
  MW395_RS25410 (MW395_25405) yggU 5044269..5044559 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  MW395_RS25415 (MW395_25410) yggT 5044556..5045122 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  MW395_RS25420 (MW395_25415) yggS 5045140..5045844 (-) 705 WP_000997806.1 pyridoxal phosphate homeostasis protein -
  MW395_RS25425 (MW395_25420) pilT 5045862..5046842 (+) 981 WP_001309723.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MW395_RS25430 (MW395_25425) ruvX 5046957..5047373 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  MW395_RS25435 (MW395_25430) yqgE 5047373..5047936 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  MW395_RS25440 (MW395_25435) gshB 5048045..5048995 (-) 951 WP_000593273.1 glutathione synthase -
  MW395_RS25445 (MW395_25440) rsmE 5049008..5049739 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MW395_RS25450 (MW395_25445) endA 5049819..5050526 (-) 708 WP_000286500.1 deoxyribonuclease I -
  MW395_RS25455 (MW395_25450) yggI 5050621..5051118 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35940.15 Da        Isoelectric Point: 5.7980

>NTDB_id=677366 MW395_RS25425 WP_001309723.1 5045862..5046842(+) (pilT) [Escherichia coli strain GN05505]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGVILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINIPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=677366 MW395_RS25425 WP_001309723.1 5045862..5046842(+) (pilT) [Escherichia coli strain GN05505]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCGTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACATTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTAGGGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A148HZL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362