Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MW401_RS05765 Genome accession   NZ_CP095510
Coordinates   1186472..1187452 (+) Length   326 a.a.
NCBI ID   WP_001309723.1    Uniprot ID   A0A148HZL8
Organism   Escherichia coli strain GN02270     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1181472..1192452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW401_RS05735 (MW401_05735) - 1181850..1182836 (-) 987 WP_000784004.1 TRAP transporter substrate-binding protein -
  MW401_RS05740 (MW401_05740) hemW 1183149..1184285 (-) 1137 WP_000239946.1 radical SAM family heme chaperone HemW -
  MW401_RS05745 (MW401_05745) rdgB 1184278..1184871 (-) 594 WP_001174736.1 XTP/dITP diphosphatase -
  MW401_RS05750 (MW401_05750) yggU 1184879..1185169 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  MW401_RS05755 (MW401_05755) yggT 1185166..1185732 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  MW401_RS05760 (MW401_05760) yggS 1185750..1186454 (-) 705 WP_000997806.1 pyridoxal phosphate homeostasis protein -
  MW401_RS05765 (MW401_05765) pilT 1186472..1187452 (+) 981 WP_001309723.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MW401_RS05770 (MW401_05770) ruvX 1187567..1187983 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  MW401_RS05775 (MW401_05775) yqgE 1187983..1188546 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  MW401_RS05780 (MW401_05780) gshB 1188655..1189605 (-) 951 WP_000593273.1 glutathione synthase -
  MW401_RS05785 (MW401_05785) rsmE 1189618..1190349 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MW401_RS05790 (MW401_05790) endA 1190429..1191136 (-) 708 WP_000286500.1 deoxyribonuclease I -
  MW401_RS05795 (MW401_05795) yggI 1191231..1191728 (-) 498 WP_059332052.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35940.15 Da        Isoelectric Point: 5.7980

>NTDB_id=677260 MW401_RS05765 WP_001309723.1 1186472..1187452(+) (pilT) [Escherichia coli strain GN02270]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGVILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINIPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=677260 MW401_RS05765 WP_001309723.1 1186472..1187452(+) (pilT) [Escherichia coli strain GN02270]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCGTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACATTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTAGGGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A148HZL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.21

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362