Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   MWN52_RS06785 Genome accession   NZ_CP095475
Coordinates   1527447..1527893 (-) Length   148 a.a.
NCBI ID   WP_286577169.1    Uniprot ID   -
Organism   Pseudoxanthomonas winnipegensis strain ZSY-33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1522447..1532893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWN52_RS06770 (MWN52_06790) - 1523251..1524114 (-) 864 WP_130523577.1 A24 family peptidase -
  MWN52_RS06775 (MWN52_06795) pilC 1524118..1525380 (-) 1263 WP_286577167.1 type II secretion system F family protein Machinery gene
  MWN52_RS06780 (MWN52_06800) pilB 1525520..1527244 (-) 1725 WP_286577168.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MWN52_RS06785 (MWN52_06805) pilA2 1527447..1527893 (-) 447 WP_286577169.1 pilin Machinery gene
  MWN52_RS06790 (MWN52_06810) pilR 1528248..1529711 (-) 1464 WP_286577171.1 sigma-54 dependent transcriptional regulator Regulator
  MWN52_RS06795 (MWN52_06815) - 1529802..1531430 (-) 1629 WP_286577172.1 PAS domain-containing sensor histidine kinase -
  MWN52_RS06800 (MWN52_06820) sucC 1531619..1532788 (+) 1170 WP_130519285.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 14797.11 Da        Isoelectric Point: 9.0866

>NTDB_id=677117 MWN52_RS06785 WP_286577169.1 1527447..1527893(-) (pilA2) [Pseudoxanthomonas winnipegensis strain ZSY-33]
MKKNMQGGFTLIELMIVVAIIAILAAIALPAYQDYVVRSRVSEAMVLADGLKVTVAENAAQGAADLGTGATLTTSADKSP
NVTSTAVDAKTGAITVTTTSKAGNGTVVLTPNAGGAALVAGTPPNGTIVWTCTGTVKQKYLPSSCKGA

Nucleotide


Download         Length: 447 bp        

>NTDB_id=677117 MWN52_RS06785 WP_286577169.1 1527447..1527893(-) (pilA2) [Pseudoxanthomonas winnipegensis strain ZSY-33]
ATGAAGAAGAACATGCAGGGCGGCTTCACCCTCATCGAACTGATGATCGTGGTCGCCATCATCGCCATCCTGGCCGCCAT
CGCGCTGCCGGCGTATCAGGACTATGTCGTGCGCTCACGCGTCTCTGAAGCCATGGTTTTGGCGGATGGCCTGAAGGTCA
CTGTTGCCGAGAATGCCGCCCAAGGCGCGGCGGACCTGGGCACCGGGGCAACTTTGACCACGTCAGCCGACAAATCCCCT
AACGTGACCAGCACCGCTGTCGACGCCAAAACCGGCGCCATTACCGTCACGACGACCTCCAAGGCCGGCAACGGCACTGT
TGTCTTGACGCCGAACGCAGGTGGCGCCGCGCTTGTGGCTGGTACTCCGCCGAACGGCACGATCGTGTGGACCTGCACTG
GTACGGTCAAGCAGAAGTACCTGCCGAGTAGCTGTAAGGGAGCGTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.748

96.622

0.5
  pilA Ralstonia pseudosolanacearum GMI1000

45.062

100

0.493
  pilA2 Legionella pneumophila strain ERS1305867

51.049

96.622

0.493
  comP Acinetobacter baylyi ADP1

44.872

100

0.473
  pilA/pilA1 Eikenella corrodens VA1

39.13

100

0.426
  pilA Pseudomonas aeruginosa PAK

40.132

100

0.412
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.462

100

0.405
  pilE Neisseria gonorrhoeae MS11

35.802

100

0.392
  pilA/pilAII Pseudomonas stutzeri DSM 10701

36.667

100

0.372
  pilA Vibrio cholerae C6706

36

100

0.365
  pilA Vibrio cholerae strain A1552

36

100

0.365
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36

100

0.365
  pilA Acinetobacter baumannii strain A118

38.028

95.946

0.365