Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MWN52_RS06780 Genome accession   NZ_CP095475
Coordinates   1525520..1527244 (-) Length   574 a.a.
NCBI ID   WP_286577168.1    Uniprot ID   -
Organism   Pseudoxanthomonas winnipegensis strain ZSY-33     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1520520..1532244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWN52_RS06765 (MWN52_06785) - 1522009..1523013 (+) 1005 WP_286577165.1 class I fructose-bisphosphate aldolase -
  MWN52_RS06770 (MWN52_06790) - 1523251..1524114 (-) 864 WP_130523577.1 A24 family peptidase -
  MWN52_RS06775 (MWN52_06795) pilC 1524118..1525380 (-) 1263 WP_286577167.1 type II secretion system F family protein Machinery gene
  MWN52_RS06780 (MWN52_06800) pilB 1525520..1527244 (-) 1725 WP_286577168.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MWN52_RS06785 (MWN52_06805) pilA2 1527447..1527893 (-) 447 WP_286577169.1 pilin Machinery gene
  MWN52_RS06790 (MWN52_06810) pilR 1528248..1529711 (-) 1464 WP_286577171.1 sigma-54 dependent transcriptional regulator Regulator
  MWN52_RS06795 (MWN52_06815) - 1529802..1531430 (-) 1629 WP_286577172.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62513.83 Da        Isoelectric Point: 5.8325

>NTDB_id=677116 MWN52_RS06780 WP_286577168.1 1525520..1527244(-) (pilB) [Pseudoxanthomonas winnipegensis strain ZSY-33]
MNAIASPNLVGITGIARRLVMDGALEEGAARTAMAEASRAKIPLAQWIQEMKLVSPAQLAAANAIEFGIPLIDASAFDPA
QNALKLVSEELLQKHQILPMFKRGNKLYVGVSDPTRMQGLDEIKFHTNLVVEPILVADDQLRRTLEAWSNASDSLAGMGG
DDEDFGSLDTAAGDEESGDTGIDAKGEDTPVVKFVNKMLVDAIRKGASDIHFEPYETDYRVRLRIDGILKTVARAPQKLN
QRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTRQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEEDQQKLFLD
AIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCTRCKRPATIPAKAL
LAEGFSQAELDEGFSVFEAVGCDECTEGYKGRVGIYQVMPMTDEIATIILEGGGAIQIAEAAQRSGIRDLRQSALWKVKQ
GLTSLAEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=677116 MWN52_RS06780 WP_286577168.1 1525520..1527244(-) (pilB) [Pseudoxanthomonas winnipegensis strain ZSY-33]
ATGAACGCAATTGCATCCCCCAACCTCGTCGGCATCACTGGAATCGCCCGTCGCCTCGTGATGGATGGCGCGCTTGAAGA
AGGCGCAGCGCGCACCGCAATGGCTGAAGCATCTCGAGCCAAGATTCCATTGGCGCAGTGGATACAAGAGATGAAGCTTG
TAAGCCCTGCACAGCTCGCTGCCGCGAACGCTATCGAGTTTGGCATCCCGTTGATTGATGCATCAGCGTTCGATCCTGCC
CAGAACGCCCTGAAACTAGTCAGCGAGGAACTGCTTCAGAAGCATCAAATCCTTCCTATGTTCAAGCGTGGCAACAAGCT
GTATGTCGGCGTCTCCGACCCGACGCGAATGCAAGGGTTGGACGAAATCAAGTTCCATACGAACCTCGTCGTCGAACCAA
TCCTAGTCGCTGATGACCAGCTACGACGGACTCTGGAGGCCTGGAGTAATGCCTCGGACTCGCTCGCCGGCATGGGCGGC
GACGACGAGGATTTCGGCAGCCTGGACACCGCGGCTGGCGATGAAGAAAGCGGCGACACGGGTATCGATGCCAAGGGAGA
AGACACACCTGTCGTCAAGTTTGTCAATAAAATGCTGGTCGATGCCATCCGCAAAGGAGCTTCCGACATCCATTTCGAGC
CTTATGAGACAGACTATCGCGTGCGCCTTCGCATCGATGGCATCCTTAAGACCGTCGCACGAGCGCCGCAGAAGCTTAAC
CAGCGCATCGCCGCGCGCCTGAAGGTCATGTCGCAGCTGGACATCGCCGAGAAGCGCGTTCCGCAGGACGGACGCATCAA
GCTCAACCTGTCAAAGACACGTCAGATCGACTTCCGCGTCAGCACCCTGCCGACGCTGTTCGGCGAGAAGATCGTGCTGC
GCATCCTAGATGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGGAGGACCAGCAGAAGCTGTTCCTGGAT
GCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCAGGCAAGACCGTGTCGCTGTACACCGCGCT
GGGCATCCTCAACGACGATACCCGCAATATCTCCACAGCCGAGGATCCGGTCGAAATACGCCTTCCCGGCGTCAACCAGG
TCCAGCAGAACAACAAGCGTGGCATGACTTTCGCCGCCGCCCTGCGCTCGTTCTTGCGACAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGATCTGGAGACGGCCGAGATCGCCGTCAAGGCGGCGCAGACGGGCCATATGGTGCTCTCGACCCT
GCACACCAACGATGCACCGCAAACCATCGCGCGACTGATGAACATGGGCATCGCGCCCTACAACATCACCAGTTCGGTTA
CCTTGGTCATTGCTCAGCGCCTGGCGCGCCGCCTGTGCACGCGCTGCAAGCGGCCCGCCACCATCCCAGCCAAGGCGTTG
CTGGCCGAGGGGTTCAGCCAAGCCGAATTGGACGAGGGATTCAGCGTCTTCGAGGCCGTGGGTTGCGACGAGTGTACCGA
GGGCTACAAAGGGCGAGTAGGCATCTACCAGGTCATGCCGATGACCGACGAGATCGCCACGATAATCCTGGAGGGCGGCG
GCGCGATACAGATTGCGGAGGCAGCACAGCGCTCTGGCATCCGTGACCTGCGCCAGTCGGCACTATGGAAGGTCAAGCAG
GGGCTGACTAGCCTAGCCGAGATCAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-148)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.766

99.129

0.563
  pilB Acinetobacter baylyi ADP1

53.741

100

0.551
  pilB Legionella pneumophila strain ERS1305867

52.12

98.606

0.514
  pilB Vibrio cholerae strain A1552

50.088

99.129

0.497
  pilF Neisseria gonorrhoeae MS11

51.048

91.463

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

49.24

91.638

0.451
  pilB Vibrio campbellii strain DS40M4

49.315

89.024

0.439
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.548

92.509

0.366