Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MWN52_RS06775 Genome accession   NZ_CP095475
Coordinates   1524118..1525380 (-) Length   420 a.a.
NCBI ID   WP_286577167.1    Uniprot ID   -
Organism   Pseudoxanthomonas winnipegensis strain ZSY-33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1519118..1530380
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWN52_RS06755 (MWN52_06775) - 1519621..1520283 (+) 663 WP_286577163.1 HAD-IA family hydrolase -
  MWN52_RS06760 (MWN52_06780) pyk 1520469..1521938 (+) 1470 WP_286577164.1 pyruvate kinase -
  MWN52_RS06765 (MWN52_06785) - 1522009..1523013 (+) 1005 WP_286577165.1 class I fructose-bisphosphate aldolase -
  MWN52_RS06770 (MWN52_06790) - 1523251..1524114 (-) 864 WP_130523577.1 A24 family peptidase -
  MWN52_RS06775 (MWN52_06795) pilC 1524118..1525380 (-) 1263 WP_286577167.1 type II secretion system F family protein Machinery gene
  MWN52_RS06780 (MWN52_06800) pilB 1525520..1527244 (-) 1725 WP_286577168.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MWN52_RS06785 (MWN52_06805) pilA2 1527447..1527893 (-) 447 WP_286577169.1 pilin Machinery gene
  MWN52_RS06790 (MWN52_06810) pilR 1528248..1529711 (-) 1464 WP_286577171.1 sigma-54 dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45175.42 Da        Isoelectric Point: 10.2648

>NTDB_id=677115 MWN52_RS06775 WP_286577167.1 1524118..1525380(-) (pilC) [Pseudoxanthomonas winnipegensis strain ZSY-33]
MAVSRSAAKKSIPVARDTKQLVTFVWQGTDKRGVKMKGEQEARNANLLRAELRGKGITPSVLKPKPKPLFGAAGKPIKQG
DIAFFSRQMATMMKSGVPIITSLEIIGGGHKNPRMKKMIEGIRADIEGGSSLSEAISKFPVQFDELYRNLVRAGEGAGVL
ETVLDTIATYKENLESLKGKIKKALFYPAMTIAVALIVSAILLIFVVPQFEAVFAGFGADLPAFTKLIVAASRVATAYWW
LALLIGGGGAFAFIFALKRSLPMQHALDRALLRVPVIGQILNNSAIARFARTLAVTFKAGVPLVEALDTVAGATGSTVYE
KAVFRIRDDVAVGYPVNMAMKQTNLFPHMVIQMTAIGEEAGALDTMLFKVAEYYEQEVNNAVDALSSLIEPAIMVILGVL
VGGMVIGMYLPIFKLGEVVG

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=677115 MWN52_RS06775 WP_286577167.1 1524118..1525380(-) (pilC) [Pseudoxanthomonas winnipegensis strain ZSY-33]
ATGGCCGTAAGCCGTTCAGCCGCCAAGAAGTCCATACCGGTCGCGCGCGACACCAAGCAGTTGGTTACCTTCGTTTGGCA
AGGGACCGACAAGCGCGGCGTCAAGATGAAAGGCGAGCAGGAGGCACGCAATGCCAATCTTTTGCGCGCGGAACTGCGCG
GAAAGGGCATTACTCCCTCCGTCCTCAAACCCAAGCCCAAACCGCTGTTCGGTGCTGCCGGAAAGCCGATCAAGCAAGGC
GATATTGCTTTTTTCAGTCGCCAGATGGCAACCATGATGAAGTCGGGTGTGCCGATCATCACGTCGCTGGAGATCATCGG
CGGGGGACACAAGAACCCCCGCATGAAGAAGATGATCGAGGGGATCAGGGCGGATATCGAAGGCGGCTCCTCACTGTCTG
AGGCAATTAGTAAGTTCCCGGTCCAATTCGACGAGCTGTATCGAAACCTTGTGCGCGCCGGCGAGGGCGCGGGTGTTCTT
GAAACCGTGCTTGACACGATTGCGACTTACAAAGAAAACCTTGAGTCTTTGAAAGGCAAGATTAAGAAGGCGCTGTTCTA
TCCCGCAATGACTATTGCGGTTGCATTGATTGTCAGCGCGATCCTGCTGATTTTTGTAGTTCCTCAGTTTGAGGCTGTTT
TTGCGGGATTTGGAGCAGACCTTCCTGCATTCACCAAACTAATCGTTGCTGCATCGAGAGTAGCGACTGCTTATTGGTGG
TTGGCGCTGTTAATTGGAGGTGGCGGTGCTTTTGCGTTCATCTTTGCTCTGAAGCGCTCATTGCCAATGCAACACGCACT
TGATCGTGCGCTACTGAGGGTTCCCGTTATCGGACAGATCCTCAACAACAGTGCCATCGCACGCTTTGCGCGTACGCTGG
CCGTGACCTTCAAGGCCGGCGTTCCGCTGGTTGAGGCACTGGATACAGTCGCGGGTGCCACGGGTAGCACCGTTTATGAG
AAAGCGGTTTTCCGCATCCGCGATGACGTAGCGGTCGGTTATCCGGTCAACATGGCAATGAAGCAGACCAACCTGTTTCC
GCATATGGTGATCCAGATGACCGCAATCGGTGAGGAGGCAGGTGCGCTGGACACGATGCTGTTCAAGGTAGCTGAGTACT
ACGAGCAGGAAGTGAACAATGCAGTCGATGCGCTGAGCAGTCTGATCGAGCCAGCCATCATGGTGATTCTGGGTGTGCTT
GTCGGCGGCATGGTCATCGGCATGTATCTGCCGATCTTCAAGCTTGGCGAAGTGGTCGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(85-208)

Type II secretion system (T2SS), protein F (T2SSF)

(289-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

53.713

96.19

0.517
  pilC Pseudomonas stutzeri DSM 10701

53.283

94.286

0.502
  pilC Acinetobacter baylyi ADP1

50.246

96.667

0.486
  pilC Acinetobacter baumannii D1279779

49.877

96.429

0.481
  pilG Neisseria gonorrhoeae MS11

43.216

94.762

0.41
  pilG Neisseria meningitidis 44/76-A

42.965

94.762

0.407
  pilC Vibrio campbellii strain DS40M4

40.441

97.143

0.393
  pilC Vibrio cholerae strain A1552

40.148

96.667

0.388