Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MRL81_RS04080 Genome accession   NZ_CP095369
Coordinates   830441..831421 (+) Length   326 a.a.
NCBI ID   WP_286554139.1    Uniprot ID   -
Organism   Citrobacter portucalensis strain 19MO02SH04     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 825441..836421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRL81_RS04050 (MRL81_04030) ansB 825956..827002 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  MRL81_RS04055 (MRL81_04035) hemW 827117..828253 (-) 1137 WP_126317427.1 radical SAM family heme chaperone HemW -
  MRL81_RS04060 (MRL81_04040) - 828246..828839 (-) 594 WP_003825414.1 XTP/dITP diphosphatase -
  MRL81_RS04065 (MRL81_04045) yggU 828847..829137 (-) 291 WP_354676385.1 DUF167 family protein YggU -
  MRL81_RS04070 (MRL81_04050) - 829134..829700 (-) 567 WP_003825417.1 YggT family protein -
  MRL81_RS04075 (MRL81_04055) - 829719..830423 (-) 705 WP_057067562.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRL81_RS04080 (MRL81_04060) pilT 830441..831421 (+) 981 WP_286554139.1 type IV pilus twitching motility protein PilT Machinery gene
  MRL81_RS04085 (MRL81_04065) ruvX 831418..831834 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  MRL81_RS04090 (MRL81_04070) - 831834..832397 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  MRL81_RS04095 (MRL81_04075) gshB 832573..833520 (-) 948 WP_003027083.1 glutathione synthase -
  MRL81_RS04100 (MRL81_04080) rsmE 833540..834271 (-) 732 WP_008785768.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MRL81_RS04105 (MRL81_04085) endA 834346..835053 (-) 708 WP_008785767.1 deoxyribonuclease I -
  MRL81_RS04110 (MRL81_04090) - 835148..835645 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35933.04 Da        Isoelectric Point: 6.5835

>NTDB_id=676536 MRL81_RS04080 WP_286554139.1 830441..831421(+) (pilT) [Citrobacter portucalensis strain 19MO02SH04]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVENLLMCWLSEQQQVQWREQGQVDFAIALAGSRRLRA
SAFAHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQHVAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=676536 MRL81_RS04080 WP_286554139.1 830441..831421(+) (pilT) [Citrobacter portucalensis strain 19MO02SH04]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGGTAGATTTTGCTATTGCGCTGGCAGGTTCCCGGAGGCTGCGCGCC
AGTGCTTTTGCTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTCCCGCTTGATTGTCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACCCTGGCGGCGATGGTAGAGTACCTTAATCAGCATGTTGCAGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAACGGGAGGTGGGCGTACACTGCGCTTCTTTTGCCGCCGGTTTACG
CGGCGCGCTGCGTGAAGATCCCGACGTCATTTTGCTGGGCGAACTGCGCGACGCGGAAACCATTCGTCTGGCATTAACGG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGATACC
TTTCCCGCACAGGAGAAAGATCCGGTGCGTAATCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAACAGGGAGGGCGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCTGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAGCAGACAGGTATGCAGACATTTGCACAAAGCCAGCAGCAGCGT
CAGGCGCAGGGACGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.152

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.152

100

0.506
  pilT Neisseria meningitidis 8013

46.667

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.364

100

0.469
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Acinetobacter baylyi ADP1

45.897

100

0.463
  pilT Acinetobacter baumannii strain A118

45.593

100

0.46
  pilT Acinetobacter baumannii D1279779

45.593

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter nosocomialis M2

45.289

100

0.457
  pilT Legionella pneumophila strain Lp02

46.855

97.546

0.457
  pilT Legionella pneumophila strain ERS1305867

46.855

97.546

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.64

97.239

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371