Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MRK34_RS04065 Genome accession   NZ_CP095364
Coordinates   831009..831989 (+) Length   326 a.a.
NCBI ID   WP_103015240.1    Uniprot ID   -
Organism   Citrobacter portucalensis strain 19MO02SH05     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 826009..836989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRK34_RS04035 (MRK34_04030) ansB 826524..827570 (+) 1047 WP_003027108.1 L-asparaginase 2 -
  MRK34_RS04040 (MRK34_04035) hemW 827685..828821 (-) 1137 WP_048236736.1 radical SAM family heme chaperone HemW -
  MRK34_RS04045 (MRK34_04040) - 828814..829407 (-) 594 WP_003825414.1 XTP/dITP diphosphatase -
  MRK34_RS04050 (MRK34_04045) yggU 829415..829705 (-) 291 WP_003027101.1 DUF167 family protein YggU -
  MRK34_RS04055 (MRK34_04050) - 829702..830268 (-) 567 WP_003825417.1 YggT family protein -
  MRK34_RS04060 (MRK34_04055) - 830287..830991 (-) 705 WP_284961013.1 YggS family pyridoxal phosphate-dependent enzyme -
  MRK34_RS04065 (MRK34_04060) pilT 831009..831989 (+) 981 WP_103015240.1 type IV pilus twitching motility protein PilT Machinery gene
  MRK34_RS04070 (MRK34_04065) ruvX 831986..832402 (-) 417 WP_008785771.1 Holliday junction resolvase RuvX -
  MRK34_RS04075 (MRK34_04070) - 832402..832965 (-) 564 WP_003027086.1 YqgE/AlgH family protein -
  MRK34_RS04080 (MRK34_04075) gshB 833142..834089 (-) 948 WP_003027083.1 glutathione synthase -
  MRK34_RS04085 (MRK34_04080) rsmE 834109..834840 (-) 732 WP_103015239.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MRK34_RS04090 (MRK34_04085) endA 834915..835622 (-) 708 WP_008785767.1 deoxyribonuclease I -
  MRK34_RS04095 (MRK34_04090) - 835717..836214 (-) 498 WP_003825440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35875.98 Da        Isoelectric Point: 6.4969

>NTDB_id=676513 MRK34_RS04065 WP_103015240.1 831009..831989(+) (pilT) [Citrobacter portucalensis strain 19MO02SH05]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIRGKVEIAPFTTPDVENLLMCWLSEQQQVQWQEQGQIDFAIALAGSRRLRA
SAFAHQQGTSLALRLLPLDCPRLDDLQTPDVIPELLRSENGLILVTGATGSGKSTTLAAMVEYLNQHVAGHILTLEDPIE
YRYTSQRCLIHQREVGVHCASFAAGLRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FSATEKDPVRNQLADSLRAVLSQKLEEDKLGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRI

Nucleotide


Download         Length: 981 bp        

>NTDB_id=676513 MRK34_RS04065 WP_103015240.1 831009..831989(+) (pilT) [Citrobacter portucalensis strain 19MO02SH05]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATACGTGGAAAAGTCGAAATCGCACCCTTTACTACGCCTGACGTGGAGAATCTGCTGATGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCAGGAACAGGGGCAGATTGATTTTGCTATTGCGCTGGCAGGTTCCCGGCGGCTGCGCGCC
AGTGCTTTTGCTCATCAACAGGGAACCTCGCTGGCGCTAAGATTGCTCCCGCTTGATTGCCCACGTTTAGACGATCTCCA
GACCCCTGACGTCATACCTGAACTGCTGCGCAGTGAAAATGGGTTAATTCTGGTGACAGGCGCTACCGGTAGCGGTAAAT
CAACGACTCTGGCGGCGATGGTAGAGTACCTGAATCAGCATGTTGCGGGGCATATTCTGACGCTGGAAGACCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCACCAACGGGAGGTGGGCGTACACTGCGCTTCTTTCGCCGCCGGTTTGCG
CGGCGCGCTGCGCGAAGATCCCGACGTCATTTTGCTGGGCGAACTGCGCGACGCGGAAACCATTCGTCTGGCATTAACGG
CAGCGGAAACCGGGCATCTGGTGCTGGCAACGTTACATACGCGAGGGGCGGCACAGGCCATCGCGCGGCTGGTGGACACC
TTTTCGGCAACAGAGAAGGATCCGGTGCGTAACCAGTTAGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCTGGGAGGACGCGTGGCGCTATTTGAGCTGCTCGTCAACACGCCAGCCGTGGGCAATTTGATTCGTGAAGGGA
AAACGCATCAGCTACCCGGCGTAATTCAAACCGGGCAACAGACAGGTATGCAGACATTTGCACAAAGTCAGCAGCAGCGT
CAGGCGCAGGGACGGATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.544

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

100

0.5
  pilT Neisseria meningitidis 8013

46.364

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.061

100

0.466
  pilT Acinetobacter baylyi ADP1

45.593

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.593

100

0.46
  pilT Acinetobacter baumannii strain A118

45.289

100

0.457
  pilT Acinetobacter baumannii D1279779

45.289

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter nosocomialis M2

44.985

100

0.454
  pilT Legionella pneumophila strain Lp02

46.541

97.546

0.454
  pilT Legionella pneumophila strain ERS1305867

46.541

97.546

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.325

97.239

0.402
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368