Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   FIS3754_RS17120 Genome accession   NZ_AP017305
Coordinates   4197167..4198294 (+) Length   375 a.a.
NCBI ID   WP_062248324.1    Uniprot ID   -
Organism   Fischerella sp. NIES-3754     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4192167..4203294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIS3754_RS17110 (FIS3754_36230) grpE 4193617..4194357 (-) 741 WP_062248319.1 nucleotide exchange factor GrpE -
  FIS3754_RS17115 (FIS3754_36240) pilB/pilB1 4195066..4197078 (+) 2013 WP_062248322.1 GspE/PulE family protein Machinery gene
  FIS3754_RS17120 (FIS3754_36250) pilT 4197167..4198294 (+) 1128 WP_062248324.1 type IV pilus twitching motility protein PilT Machinery gene
  FIS3754_RS17125 (FIS3754_36260) - 4198412..4199632 (+) 1221 WP_062248326.1 type II secretion system F family protein -
  FIS3754_RS17130 (FIS3754_36270) - 4199727..4200275 (+) 549 WP_062248329.1 hypothetical protein -
  FIS3754_RS17135 (FIS3754_36280) - 4200426..4201448 (-) 1023 WP_062248331.1 saccharopine dehydrogenase-like oxidoreductase -
  FIS3754_RS17140 (FIS3754_36290) tnpB 4201480..4202667 (-) 1188 WP_062248333.1 IS200/IS605 family element RNA-guided endonuclease TnpB -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 40994.34 Da        Isoelectric Point: 7.1092

>NTDB_id=67647 FIS3754_RS17120 WP_062248324.1 4197167..4198294(+) (pilT) [Fischerella sp. NIES-3754]
MEMMIEDLMEQMIEMGGSDMHLSAGLPPYFRISGKLTPIGEEPLSGDQCQRLIFSMLNNSQRKTLEQNWELDCSYGVKGL
ARFRVNVYKERGSYAACLRALSSKIPNFDKLGLPDVVREMSEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPIEFVYEPIKSLIHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHEKQTQVRVQLSNSLVAVFSQTLVPRKNPKPGEFGRVMAQEIMIVTPAISNLIREGKTAQIYSSIQTGGKLGM
QTLEKVLADLYKAGTISFEAAMSKTSKPDEVQRLIGPAAPPAAGKVGTAGTVKAH

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=67647 FIS3754_RS17120 WP_062248324.1 4197167..4198294(+) (pilT) [Fischerella sp. NIES-3754]
ATGGAAATGATGATTGAAGATTTAATGGAGCAAATGATTGAAATGGGTGGTTCGGATATGCATTTATCCGCTGGTTTGCC
CCCTTATTTTCGCATCAGTGGCAAACTGACGCCCATTGGCGAAGAGCCTTTGTCTGGGGATCAGTGCCAAAGGTTGATTT
TTAGTATGCTCAATAACTCCCAACGCAAAACATTAGAGCAAAACTGGGAATTAGATTGCTCTTATGGTGTCAAGGGATTA
GCACGTTTCCGCGTCAATGTTTATAAAGAACGCGGTTCTTATGCAGCTTGTTTGCGGGCATTAAGTTCTAAAATACCTAA
TTTTGATAAATTAGGTTTGCCCGATGTAGTCAGAGAAATGTCGGAAAAGCCTAGAGGATTAATTCTAGTGACAGGCCCTA
CTGGTTCTGGGAAAACAACTACTCTGGCGGCAATGATTGATTTAATTAACCGCACACGGGCAGAACATATTTTGACAGTT
GAAGATCCAATTGAGTTTGTTTATGAACCAATTAAAAGTCTGATTCATCAAAGGCAATTGGGTGAAGATACAAAGAGTTT
TGCTAATGCTTTGAAAGCTGCTCTTAGAGAAGATCCAGATATTATTCTGGTAGGAGAAATGCGGGACTTAGAAACTATTT
CCTTAGCTATTTCTGCTGCTGAAACCGGACACTTAGTATTTGGTACTTTACATACTAGTTCGGCAGCACAAACTGTTGAC
CGAATTATTGACGTTTTTCCCCATGAAAAACAAACTCAGGTAAGGGTACAGTTATCTAACTCGTTAGTTGCGGTATTTAG
CCAAACTTTAGTTCCGAGAAAGAATCCTAAACCTGGTGAATTTGGTCGGGTAATGGCACAGGAAATAATGATAGTTACTC
CGGCTATTTCTAACTTAATTCGTGAAGGCAAAACAGCACAAATTTACTCATCTATTCAGACAGGTGGTAAATTAGGTATG
CAAACTCTGGAGAAGGTTTTAGCTGATTTATATAAAGCGGGAACTATTTCTTTTGAAGCAGCAATGTCTAAAACTTCTAA
GCCAGATGAAGTGCAACGTCTTATTGGCCCTGCTGCACCACCTGCTGCTGGAAAAGTAGGTACTGCTGGAACTGTAAAAG
CACATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(45-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.755

92.533

0.507
  pilT Pseudomonas stutzeri DSM 10701

54.179

92.533

0.501
  pilT Acinetobacter baylyi ADP1

53.602

92.533

0.496
  pilT Legionella pneumophila strain Lp02

53.314

92.533

0.493
  pilT Legionella pneumophila strain ERS1305867

53.314

92.533

0.493
  pilT Acinetobacter baumannii D1279779

52.161

92.533

0.483
  pilT Acinetobacter baumannii strain A118

52.161

92.533

0.483
  pilT Acinetobacter nosocomialis M2

52.161

92.533

0.483
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.78

98.4

0.48
  pilT Neisseria gonorrhoeae MS11

51.032

90.4

0.461
  pilT Vibrio cholerae strain A1552

50.292

91.2

0.459
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.292

91.2

0.459
  pilT Neisseria meningitidis 8013

50.737

90.4

0.459
  pilU Acinetobacter baylyi ADP1

38.527

94.133

0.363
  pilU Pseudomonas stutzeri DSM 10701

38.527

94.133

0.363

Multiple sequence alignment