Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   FIS3754_RS17115 Genome accession   NZ_AP017305
Coordinates   4195066..4197078 (+) Length   670 a.a.
NCBI ID   WP_062248322.1    Uniprot ID   A0A0S3TRI5
Organism   Fischerella sp. NIES-3754     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4190066..4202078
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIS3754_RS17105 (FIS3754_36220) dnaK 4191370..4193334 (-) 1965 WP_062248317.1 molecular chaperone DnaK -
  FIS3754_RS17110 (FIS3754_36230) grpE 4193617..4194357 (-) 741 WP_062248319.1 nucleotide exchange factor GrpE -
  FIS3754_RS17115 (FIS3754_36240) pilB/pilB1 4195066..4197078 (+) 2013 WP_062248322.1 GspE/PulE family protein Machinery gene
  FIS3754_RS17120 (FIS3754_36250) pilT 4197167..4198294 (+) 1128 WP_062248324.1 type IV pilus twitching motility protein PilT Machinery gene
  FIS3754_RS17125 (FIS3754_36260) - 4198412..4199632 (+) 1221 WP_062248326.1 type II secretion system F family protein -
  FIS3754_RS17130 (FIS3754_36270) - 4199727..4200275 (+) 549 WP_062248329.1 hypothetical protein -
  FIS3754_RS17135 (FIS3754_36280) - 4200426..4201448 (-) 1023 WP_062248331.1 saccharopine dehydrogenase-like oxidoreductase -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74725.55 Da        Isoelectric Point: 5.2459

>NTDB_id=67646 FIS3754_RS17115 WP_062248322.1 4195066..4197078(+) (pilB/pilB1) [Fischerella sp. NIES-3754]
MTNSSPQRRSTALSTRAEFSPFGNKLVQSGFVNSEQMRQALIESRKSGIPLTEVLESVSGRKLSPELLRQYKKQQLFELK
ILYGVESLDPEISSVGSTTVAHLIDTLIPVDVCRRHRLVPLSKNEDQNPPSVLVAMVDPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQLLINVYLDDLAVRQKHLEQEKYTDINQDLENLENLNLDDAPEESEADLGAAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIIANLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSATQLGLDKLITDPETLHIVQEMVSRPFGLILVTGPTGSGKTTSLYSALAERNEPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMISSSLIGVLAQRLVRRVCPDCRIPYTPTVEELARYGLSASQEVGITFYKANTLTAEQIQAAKTQGHICPTCNG
VGYKGRCGVYEVMRISENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGLTTLEEVERVTFTDTGLEAELKAKR
KTSLTCRTCNAGLRPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=67646 FIS3754_RS17115 WP_062248322.1 4195066..4197078(+) (pilB/pilB1) [Fischerella sp. NIES-3754]
ATGACTAACTCGTCACCACAACGGCGTAGTACAGCTCTGTCTACCAGAGCAGAATTTTCACCCTTCGGTAACAAACTTGT
ACAATCTGGCTTTGTCAACAGCGAACAAATGAGACAGGCGCTGATTGAAAGCCGCAAGTCTGGTATCCCTTTAACAGAAG
TACTGGAATCAGTATCGGGGCGAAAATTATCACCAGAGTTACTCAGGCAGTACAAAAAACAGCAGTTATTTGAACTTAAA
ATACTTTACGGCGTAGAATCCCTCGATCCGGAAATTAGTTCAGTAGGCAGCACCACAGTAGCCCACCTGATTGATACCCT
CATTCCAGTAGATGTTTGTCGTCGTCACCGCTTAGTACCACTGTCGAAAAACGAGGATCAAAACCCGCCCTCTGTTCTAG
TGGCGATGGTTGATCCAGATAATCTAGAAGCTTCGGATGACTTAAACCGGATTTTGCGCCCCCAAGGCTTAGCCTTGCAG
CGTATGGTAATTACCCAAGAAGATTATCAACTGTTAATCAATGTATATCTGGATGATTTAGCCGTTCGGCAAAAGCATTT
AGAACAAGAAAAATATACAGACATCAATCAAGATTTAGAAAATCTAGAAAATCTTAACCTAGATGACGCCCCAGAAGAAT
CGGAGGCTGACTTGGGGGCGGCGATGAAGGGTGCTGAAGATGCACCTGTGATTAATCTGGTTAACAGAATCCTTGCTAAA
GCACTACATGAGAAAGTATCTGATATTCACATAGAACCGCAAGAAGAAAACCTCCGCATTCGCTTTCGTAAAGATGGGGT
TTTGCGCGAGGCTTTCGACCCTTTGCCAAAAAAAATCATTCCTGCTGTGACAGCGCGGTTTAAAATCATCGCCAATTTAG
ATATTGCTGAACGACGCCTACCTCAAGACGGACGGATTAGGCGGTTGTTCGAGGGGCGTAAAGTTGACTTCCGTGTAAAT
ACTTTGCCGAGTCGCTACGGGGAAAAGGTAGTATTGCGGATTTTGGATAACTCTGCCACGCAACTGGGTTTGGATAAGTT
AATTACCGATCCAGAAACGTTGCATATTGTTCAAGAAATGGTTAGCCGTCCTTTTGGCTTAATTTTAGTAACCGGGCCAA
CAGGTTCTGGTAAGACAACTTCGCTGTATTCAGCACTAGCAGAAAGAAACGAGCCGGGAATTAATATCAGTACTGTAGAA
GATCCAATTGAATATAGCTTGCCGGGAATTACTCAAGTACAGGTGATTCGGGAAAAAGGGTTAGATTTTGCGACTGCATT
GCGGGCATTTTTGCGGCAAGATCCAGATGTACTACTGGTGGGTGAGACACGAGATAAGGAAACGGCAAAAACAGCGATTG
AAGCAGCATTAACTGGTCACTTAGTATTGACTACTTTACATACTAATGATGCCCCTGGTGCGATCGCTCGTTTAGGAGAA
ATGGGTATTGAACCTTTCATGATTTCCAGTTCATTGATTGGTGTCTTGGCCCAGCGTTTGGTACGGCGTGTGTGTCCTGA
CTGTCGCATTCCCTACACACCCACTGTAGAAGAACTCGCTCGTTATGGTTTGTCGGCTTCCCAAGAAGTAGGAATAACCT
TTTATAAAGCTAATACTCTGACCGCAGAGCAAATTCAAGCAGCCAAAACTCAAGGTCATATTTGTCCCACCTGTAATGGC
GTAGGCTACAAAGGACGTTGTGGTGTCTATGAGGTAATGCGAATTAGTGAAAATCTGCAAACCCTCATTAACGAAGAAGC
ACCGACAGAACGCATTAAAGAAGTAGCAGTAGAAGAGGGTATGAAAACCCTACTGGCGTACAGCTTGGACTTAGTACGCC
AAGGTTTAACCACCCTAGAAGAGGTAGAGCGAGTCACCTTTACTGACACAGGCTTAGAAGCAGAACTAAAAGCCAAACGC
AAGACTAGTCTGACCTGTCGGACTTGTAATGCTGGGTTGCGCCCAGAATGGTTGGATTGTCCGTATTGTATGACGCCTCG
TTTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0S3TRI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.327

100

0.655
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.771

91.194

0.363
  pilB Vibrio campbellii strain DS40M4

40.066

90.149

0.361

Multiple sequence alignment