Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   MUU75_RS15770 Genome accession   NZ_CP095186
Coordinates   3378270..3378683 (-) Length   137 a.a.
NCBI ID   WP_280640360.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain F31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3362526..3378239 3378270..3378683 flank 31


Gene organization within MGE regions


Location: 3362526..3378683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUU75_RS15700 (MUU75_15700) - 3362991..3363416 (+) 426 WP_245092010.1 transposase -
  MUU75_RS15705 (MUU75_15705) coaE 3363840..3364457 (-) 618 WP_245092012.1 dephospho-CoA kinase -
  MUU75_RS15710 (MUU75_15710) pilD 3364491..3365354 (-) 864 WP_187573334.1 A24 family peptidase Machinery gene
  MUU75_RS15715 (MUU75_15715) pilC 3365364..3366620 (-) 1257 WP_245094214.1 type II secretion system F family protein Machinery gene
  MUU75_RS15720 (MUU75_15720) pilB 3366918..3368659 (-) 1742 Protein_3110 type IV-A pilus assembly ATPase PilB -
  MUU75_RS15725 (MUU75_15725) - 3368874..3369720 (+) 847 Protein_3111 glycosyltransferase family 2 protein -
  MUU75_RS15730 (MUU75_15730) - 3369735..3370964 (+) 1230 WP_245092023.1 glycosyltransferase family 4 protein -
  MUU75_RS15735 (MUU75_15735) - 3371004..3372194 (+) 1191 WP_245092025.1 oligosaccharide flippase family protein -
  MUU75_RS15740 (MUU75_15740) - 3372175..3373305 (-) 1131 WP_245092027.1 glycosyltransferase -
  MUU75_RS15745 (MUU75_15745) - 3373312..3374181 (-) 870 WP_245092029.1 FkbM family methyltransferase -
  MUU75_RS15750 (MUU75_15750) - 3374178..3374963 (-) 786 WP_245092031.1 class I SAM-dependent methyltransferase -
  MUU75_RS15755 (MUU75_15755) - 3375482..3376159 (+) 678 WP_245106475.1 glycosyltransferase -
  MUU75_RS15760 (MUU75_15760) - 3376216..3377994 (-) 1779 WP_245106477.1 hypothetical protein -
  MUU75_RS15765 (MUU75_15765) - 3377976..3378239 (-) 264 WP_245106479.1 DUF4559 domain-containing protein -
  MUU75_RS15770 (MUU75_15770) pilA2 3378270..3378683 (-) 414 WP_280640360.1 pilin Machinery gene

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14325.49 Da        Isoelectric Point: 4.9006

>NTDB_id=676020 MUU75_RS15770 WP_280640360.1 3378270..3378683(-) (pilA2) [Pseudoxanthomonas mexicana strain F31]
MKKNMQGFTLIELMIVIAILGILLAIAIPAYQDYTVRAKVSEGLNLAASPKLAVAEYAISNNAWPTEAQSGYTFAATSIV
NDISIGANGQIVITYQTAEVPGGGTINLTPDTTVAGVTWVCAAGSLPTKYTPSSCRN

Nucleotide


Download         Length: 414 bp        

>NTDB_id=676020 MUU75_RS15770 WP_280640360.1 3378270..3378683(-) (pilA2) [Pseudoxanthomonas mexicana strain F31]
ATGAAGAAGAACATGCAGGGCTTCACCCTGATCGAACTGATGATCGTCATCGCCATCCTGGGCATCCTGCTGGCCATCGC
GATTCCGGCCTATCAGGATTACACCGTCCGCGCCAAGGTCTCGGAAGGTCTGAACCTGGCAGCCTCGCCGAAGCTGGCCG
TCGCCGAGTACGCGATTTCCAACAACGCGTGGCCGACCGAAGCTCAGTCGGGCTACACCTTCGCCGCCACCTCGATCGTC
AACGACATTTCGATCGGCGCCAATGGCCAGATCGTCATCACCTATCAAACTGCGGAAGTCCCGGGCGGCGGCACCATCAA
CCTGACGCCGGACACCACCGTGGCTGGCGTGACCTGGGTCTGCGCGGCTGGCAGCCTGCCGACCAAGTACACCCCGTCCA
GCTGCCGCAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)

Pilin (bacterial filament) (Pilin)

(36-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

62.5

99.27

0.62
  pilA2 Legionella pneumophila strain ERS1305867

62.5

99.27

0.62
  pilA Ralstonia pseudosolanacearum GMI1000

43.976

100

0.533
  comP Acinetobacter baylyi ADP1

47.917

100

0.504
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.052

100

0.496
  pilE Neisseria gonorrhoeae strain FA1090

37.975

100

0.438
  pilE Neisseria gonorrhoeae MS11

37.58

100

0.431
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.426

100

0.416
  pilA/pilA1 Eikenella corrodens VA1

35.897

100

0.409
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.732

100

0.401
  pilA Acinetobacter baumannii strain A118

37.681

100

0.38
  pilA Haemophilus influenzae Rd KW20

42.276

89.781

0.38
  pilA Vibrio parahaemolyticus RIMD 2210633

38.346

97.08

0.372
  pilA Vibrio cholerae C6706

34.483

100

0.365
  pilA Vibrio cholerae strain A1552

34.483

100

0.365
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

34.483

100

0.365