Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NOS3756_RS24600 Genome accession   NZ_AP017295
Coordinates   5929766..5930890 (-) Length   374 a.a.
NCBI ID   WP_067773997.1    Uniprot ID   -
Organism   Nostoc sp. NIES-3756     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5924766..5935890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOS3756_RS24570 (NOS3756_50680) - 5924941..5925324 (+) 384 WP_067773984.1 PH domain-containing protein -
  NOS3756_RS31275 (NOS3756_50690) - 5925306..5925455 (-) 150 WP_171843544.1 hypothetical protein -
  NOS3756_RS24575 (NOS3756_50700) yidC 5925535..5926677 (+) 1143 WP_067773987.1 membrane protein insertase YidC -
  NOS3756_RS24580 (NOS3756_50710) - 5926681..5927217 (+) 537 WP_067773990.1 R3H domain-containing nucleic acid-binding protein -
  NOS3756_RS24585 (NOS3756_50720) - 5927342..5927851 (+) 510 WP_067776204.1 YceD family protein -
  NOS3756_RS24590 (NOS3756_50730) - 5927934..5928485 (-) 552 WP_067773993.1 hypothetical protein -
  NOS3756_RS24595 (NOS3756_50740) - 5928485..5929696 (-) 1212 WP_067773995.1 type II secretion system F family protein -
  NOS3756_RS24600 (NOS3756_50750) pilT 5929766..5930890 (-) 1125 WP_067773997.1 type IV pilus twitching motility protein PilT Machinery gene
  NOS3756_RS24605 (NOS3756_50760) pilB/pilB1 5930968..5932980 (-) 2013 WP_067774000.1 GspE/PulE family protein Machinery gene
  NOS3756_RS24610 (NOS3756_50770) grpE 5933382..5934128 (+) 747 WP_067774003.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40944.32 Da        Isoelectric Point: 7.0974

>NTDB_id=67598 NOS3756_RS24600 WP_067773997.1 5929766..5930890(-) (pilT) [Nostoc sp. NIES-3756]
MEMMIEDLMEQMIEMGGSDLHLSAGLPPYFRISGKLTPIGEEVLTADQCQRLIFSMLNNSQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREMCDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSASQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRIMAQEIMIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYKAGTISFEAAMSKTSKPDEIQRLIGGSAPPPAAGAKPGAVKAH

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=67598 NOS3756_RS24600 WP_067773997.1 5929766..5930890(-) (pilT) [Nostoc sp. NIES-3756]
ATGGAAATGATGATCGAAGATTTGATGGAGCAGATGATTGAAATGGGTGGCTCGGATCTACACCTATCCGCAGGTTTACC
ACCTTATTTCCGCATTAGTGGGAAGCTCACACCAATAGGTGAAGAGGTACTCACTGCTGACCAGTGCCAAAGGCTGATTT
TTAGTATGCTCAATAACAGCCAACGCAAAACCTTAGAACAGACTTGGGAGTTAGATTGTTCTTATGGTGTCAAGGGTTTG
GCTCGTTTCCGGGTGAATGTATATAAAGAGCGTGGTGCTTATGCTGCTTGTTTACGGGCGCTGAGTTCTAAAATTCCTAA
CTTTGAAAAATTGGGTTTGCCTGATGTAGTACGGGAAATGTGCGACAAACCCAGAGGTTTGATTCTCGTAACAGGGCCAA
CAGGTTCTGGTAAAACGACCACTCTAGCGGCAATGATTGATTTAATTAACCGCACTAAAGCAGAGCATATTCTTACCGTA
GAAGACCCCATTGAATTTGTTTACGAACCAATTAAAAGCTTAGTTCACCAACGTCAATTAGGTGAAGATACAAAAAGTTT
TGCCAATGCTTTAAAAGCTGCATTACGGGAAGATCCAGATATTATTCTGGTGGGTGAAATGCGTGATTTGGAAACTATTT
CTTTGGCAATTTCCGCAGCAGAAACCGGACACTTAGTCTTTGGGACACTACACACCAGTTCAGCCTCTCAAACTGTTGAC
CGGATTATTGACGTTTTTCCTCATGAAAGACAAACCCAAGTGCGGGTGCAGTTATCGAACTCTTTAGTTGCAGTATTTAG
CCAAACTTTAGTACCTAAGAAAAACCCCAAACCGGGTGAATATGGCCGCATCATGGCGCAGGAAATTATGATTGTTACTC
CTGCTATTTCTAACCTAATTCGTGAAGGTAAAACCTCCCAGATTTACTCAGCAATTCAAACTGGCGGTAAGTTGGGAATG
CAAACTTTAGAGAAGGTTTTAGCTGATTATTACAAAGCAGGAACTATCTCTTTTGAAGCTGCGATGTCTAAAACCTCTAA
ACCAGATGAAATTCAACGTCTCATTGGTGGTTCTGCTCCACCACCCGCCGCAGGAGCTAAACCTGGAGCAGTCAAAGCAC
ACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.728

93.316

0.511
  pilT Pseudomonas stutzeri DSM 10701

54.155

93.316

0.505
  pilT Legionella pneumophila strain Lp02

53.026

92.781

0.492
  pilT Acinetobacter baylyi ADP1

53.026

92.781

0.492
  pilT Legionella pneumophila strain ERS1305867

53.026

92.781

0.492
  pilT Acinetobacter baumannii strain A118

52.161

92.781

0.484
  pilT Acinetobacter nosocomialis M2

52.161

92.781

0.484
  pilT Acinetobacter baumannii D1279779

52.161

92.781

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.956

98.128

0.471
  pilT Neisseria gonorrhoeae MS11

48.424

93.316

0.452
  pilT Vibrio cholerae strain A1552

49.415

91.444

0.452
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.415

91.444

0.452
  pilT Neisseria meningitidis 8013

48.138

93.316

0.449
  pilU Pseudomonas stutzeri DSM 10701

38.527

94.385

0.364
  pilU Acinetobacter baylyi ADP1

38.244

94.385

0.361

Multiple sequence alignment