Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NOS3756_RS24605 Genome accession   NZ_AP017295
Coordinates   5930968..5932980 (-) Length   670 a.a.
NCBI ID   WP_067774000.1    Uniprot ID   -
Organism   Nostoc sp. NIES-3756     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5925968..5937980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOS3756_RS24580 (NOS3756_50710) - 5926681..5927217 (+) 537 WP_067773990.1 R3H domain-containing nucleic acid-binding protein -
  NOS3756_RS24585 (NOS3756_50720) - 5927342..5927851 (+) 510 WP_067776204.1 YceD family protein -
  NOS3756_RS24590 (NOS3756_50730) - 5927934..5928485 (-) 552 WP_067773993.1 hypothetical protein -
  NOS3756_RS24595 (NOS3756_50740) - 5928485..5929696 (-) 1212 WP_067773995.1 type II secretion system F family protein -
  NOS3756_RS24600 (NOS3756_50750) pilT 5929766..5930890 (-) 1125 WP_067773997.1 type IV pilus twitching motility protein PilT Machinery gene
  NOS3756_RS24605 (NOS3756_50760) pilB/pilB1 5930968..5932980 (-) 2013 WP_067774000.1 GspE/PulE family protein Machinery gene
  NOS3756_RS24610 (NOS3756_50770) grpE 5933382..5934128 (+) 747 WP_067774003.1 nucleotide exchange factor GrpE -
  NOS3756_RS24615 (NOS3756_50780) dnaK 5934416..5936386 (+) 1971 WP_067774006.1 molecular chaperone DnaK -
  NOS3756_RS31280 (NOS3756_50790) - 5936518..5936655 (+) 138 WP_171843545.1 hypothetical protein -
  NOS3756_RS24620 (NOS3756_50800) dnaJ 5936829..5937956 (+) 1128 WP_067774009.1 molecular chaperone DnaJ -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74887.66 Da        Isoelectric Point: 5.2490

>NTDB_id=67599 NOS3756_RS24605 WP_067774000.1 5930968..5932980(-) (pilB/pilB1) [Nostoc sp. NIES-3756]
MTQSSPQRRSTALTTRTEFSPFGTKLVQSGYVNTEQMRQALIESRKSGRPLTDVLESITGRQLSPEFLRQYKKQQLFELK
ILYGVESLDPEVNSVGNTMVGNLIETLIPVDICRRHRLVPLSRNDEHNPPYILVAMVSPDNLEASDDLNRILRPQGLSLQ
RMVITQEDYQQLINQYLDELAVRQKHLEQEKFTDINQDLENLGNLDLEDAPDDMEADLGSAMKGAEDAPVINLVNRILAK
ALHEKVSDIHVEPQEENMRIRFRKDGVLREAFPPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVCLRILDNSSTQLGLDKLITDPETLNIVKDMVSKPFGLILVTGPTGSGKTTSLYSALSEKNSPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLMRRVCPDCRIAYNPTPEELARYGLSASQEISVTFYKANTVPSEAIAEAKAKNELCPACNG
AGYRGRCGVYEVMKISENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGSTTLEEVERVTFTDTGLEAELKAKR
KSGLTCRTCSAQLQPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=67599 NOS3756_RS24605 WP_067774000.1 5930968..5932980(-) (pilB/pilB1) [Nostoc sp. NIES-3756]
ATGACTCAATCGTCACCACAACGGCGCAGCACCGCTCTAACTACGAGAACAGAGTTTTCACCTTTCGGTACTAAATTAGT
ACAATCTGGCTACGTTAATACCGAACAGATGAGGCAAGCGCTGATTGAAAGTCGCAAGTCTGGTAGACCCTTAACAGACG
TTTTGGAGTCAATCACTGGACGACAGCTATCACCCGAGTTCCTCAGACAGTATAAGAAACAGCAATTATTTGAATTAAAA
ATCCTATACGGTGTTGAATCTCTCGATCCGGAGGTCAACAGTGTGGGTAACACGATGGTGGGTAATCTGATTGAAACCCT
CATCCCAGTAGATATTTGTCGCCGTCACCGTTTAGTACCGCTATCTAGGAATGATGAACACAACCCGCCCTACATTTTAG
TGGCGATGGTTTCTCCAGATAATTTGGAAGCGTCAGATGACTTAAACCGCATCTTGCGCCCTCAAGGTTTGTCATTACAG
CGTATGGTAATTACCCAGGAAGATTACCAACAGCTAATTAACCAATATTTGGATGAGTTGGCAGTACGGCAAAAGCACCT
GGAACAAGAAAAATTTACAGATATTAATCAGGATTTAGAAAATCTCGGCAATCTCGACCTAGAAGATGCGCCTGACGATA
TGGAGGCTGATTTAGGTTCAGCAATGAAAGGTGCGGAAGATGCGCCGGTCATTAACCTTGTCAATAGAATTTTGGCAAAA
GCATTGCACGAGAAGGTTTCCGATATCCATGTGGAACCGCAAGAAGAAAATATGCGTATTCGCTTTCGCAAGGATGGTGT
ACTAAGGGAGGCTTTTCCACCTCTACCGAAAAAAATTATCCCTGCGGTAACGGCGAGATTTAAAATTATCTCTAACTTAG
ATATTGCCGAGCGACGCTTACCCCAAGACGGACGTATTCGCCGGATGTTTGAAGGACGTAAGGTAGACTTCCGAGTTAAT
ACCTTACCTAGTCGCTATGGAGAAAAGGTTTGTCTGCGGATTTTGGACAACTCTTCTACCCAATTGGGGTTGGATAAGTT
GATTACTGACCCAGAAACTTTAAATATTGTCAAAGATATGGTCAGTAAGCCCTTTGGTTTGATTTTAGTAACGGGGCCGA
CTGGTTCAGGTAAGACGACCTCCTTATATTCGGCGCTATCGGAGAAGAACTCACCAGGGATTAACATTAGTACTGTAGAA
GACCCGATTGAATATAGTTTGCCAGGGATTACCCAGGTGCAGGTAATACGGGAGAAGGGACTAGATTTTGCCACGGCGTT
ACGGGCATTTTTGCGACAAGACCCGGATGTGCTACTGGTAGGTGAAACGCGAGATAAAGAAACAGCAAAAACAGCGATTG
AAGCTGCTTTAACGGGTCACTTGGTATTAACTACATTACATACCAACGATGCTCCTGGTGCGATCGCTCGTTTGGGAGAA
ATGGGTATTGAACCATTCATGGTTTCCAGTTCTCTAATTGGGGTATTGGCACAACGTCTGATGCGGCGTGTGTGTCCTGA
TTGCCGGATTGCTTACAACCCCACACCCGAAGAACTTGCCCGTTACGGGCTGTCTGCTTCTCAGGAAATTTCTGTCACTT
TTTATAAGGCTAACACCGTACCATCAGAAGCGATCGCTGAAGCTAAAGCCAAAAATGAGCTTTGCCCAGCTTGCAATGGT
GCAGGCTACAGAGGACGTTGCGGAGTCTATGAAGTGATGAAGATTTCCGAAAATCTGCAAACTCTCATCAACGAAGAAGC
ACCTACAGAACGTATCAAAGAAGTAGCCGTAGAAGAAGGGATGAAAACTTTGTTGGCTTATAGCTTAGACCTAGTGCGCC
AAGGTTCCACCACCTTAGAAGAAGTAGAACGGGTAACATTCACAGACACAGGTTTAGAAGCTGAGTTAAAAGCCAAACGC
AAGAGCGGTCTTACCTGCCGGACTTGCAGCGCCCAACTACAACCAGAATGGCTAGATTGTCCCTACTGTATGACACCGAG
ATTTCAAGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(64-179)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.327

100

0.655

Multiple sequence alignment