Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MUY32_RS19470 Genome accession   NZ_CP095180
Coordinates   4042642..4043622 (-) Length   326 a.a.
NCBI ID   WP_248274563.1    Uniprot ID   -
Organism   Enterobacter roggenkampii strain Ek140     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4037642..4048622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUY32_RS19440 (MUY32_19440) - 4038523..4039020 (+) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -
  MUY32_RS19445 (MUY32_19445) endA 4039115..4039822 (+) 708 WP_021242074.1 deoxyribonuclease I -
  MUY32_RS19450 (MUY32_19450) rsmE 4039874..4040605 (+) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MUY32_RS19455 (MUY32_19455) gshB 4040625..4041572 (+) 948 WP_008499751.1 glutathione synthase -
  MUY32_RS19460 (MUY32_19460) - 4041655..4042215 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  MUY32_RS19465 (MUY32_19465) ruvX 4042215..4042631 (+) 417 WP_023326041.1 Holliday junction resolvase RuvX -
  MUY32_RS19470 (MUY32_19470) pilT 4042642..4043622 (-) 981 WP_248274563.1 type IV pilus twitching motility protein PilT Machinery gene
  MUY32_RS19475 (MUY32_19475) - 4043640..4044341 (+) 702 WP_008499756.1 YggS family pyridoxal phosphate-dependent enzyme -
  MUY32_RS19480 (MUY32_19480) - 4044363..4044929 (+) 567 WP_008499757.1 YggT family protein -
  MUY32_RS19485 (MUY32_19485) yggU 4044926..4045222 (+) 297 WP_008499758.1 DUF167 family protein YggU -
  MUY32_RS19490 (MUY32_19490) - 4045226..4045819 (+) 594 WP_032664352.1 XTP/dITP diphosphatase -
  MUY32_RS19495 (MUY32_19495) hemW 4045812..4046960 (+) 1149 WP_025912469.1 radical SAM family heme chaperone HemW -
  MUY32_RS19500 (MUY32_19500) - 4047151..4047867 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  MUY32_RS19505 (MUY32_19505) - 4047924..4048250 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35512.43 Da        Isoelectric Point: 6.6962

>NTDB_id=675966 MUY32_RS19470 WP_248274563.1 4042642..4043622(-) (pilT) [Enterobacter roggenkampii strain Ek140]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPSPDLGALLKAWLNDEQQGIWWANGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRAIPELLTNASGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQDGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSQAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=675966 MUY32_RS19470 WP_248274563.1 4042642..4043622(-) (pilT) [Enterobacter roggenkampii strain Ek140]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCAGAACCGGCCGCCTTGAACCGGCACCGTTTCCTTCTCCGGACCTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAACGGGCAGGTGGATTTTGCCGCTACGGTGGCGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGAGTCTCTGTGACGCTGCGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCGCTGGG
CGCGCCGAGGGCAATCCCGGAACTGTTAACCAATGCGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATTCTCACGCTCGAAGATCCGGTGGAG
TTTATCTATCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATCGGCCAGCACAGCCCGTCATTTGCCGATGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTGATTTTGCTGGGAGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCGGAGACCGGGCACCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAAGCTGCTTCC
TGACTTACAGGACGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACCGCGGCGGCGGCGAATCTGATACGAGAAGGAA
AAACGTGGCAGCTGCCCGGCATTATTCAAACCGGACAGCAGGCAGGAATGCAGAACTTTGACCAAAGCCAGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Neisseria meningitidis 8013

48.125

98.16

0.472
  pilT Pseudomonas stutzeri DSM 10701

48.265

97.239

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Neisseria gonorrhoeae MS11

47.812

98.16

0.469
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

47.335

97.853

0.463
  pilT Acinetobacter baumannii D1279779

47.335

97.853

0.463
  pilT Acinetobacter baumannii strain A118

47.335

97.853

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.201

97.853

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.275

100

0.399
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362