Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MUY33_RS19805 Genome accession   NZ_CP095170
Coordinates   4085059..4086039 (-) Length   326 a.a.
NCBI ID   WP_032653323.1    Uniprot ID   -
Organism   Enterobacter roggenkampii strain K666     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4080059..4091039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUY33_RS19775 (MUY33_19775) - 4080940..4081437 (+) 498 WP_021242073.1 SprT family zinc-dependent metalloprotease -
  MUY33_RS19780 (MUY33_19780) endA 4081532..4082239 (+) 708 WP_021242074.1 deoxyribonuclease I -
  MUY33_RS19785 (MUY33_19785) rsmE 4082291..4083022 (+) 732 WP_032653317.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MUY33_RS19790 (MUY33_19790) gshB 4083042..4083989 (+) 948 WP_023326040.1 glutathione synthase -
  MUY33_RS19795 (MUY33_19795) - 4084072..4084632 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  MUY33_RS19800 (MUY33_19800) ruvX 4084632..4085048 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  MUY33_RS19805 (MUY33_19805) pilT 4085059..4086039 (-) 981 WP_032653323.1 type IV pilus twitching motility protein PilT Machinery gene
  MUY33_RS19810 (MUY33_19810) - 4086057..4086758 (+) 702 WP_008499756.1 YggS family pyridoxal phosphate-dependent enzyme -
  MUY33_RS19815 (MUY33_19815) - 4086780..4087346 (+) 567 WP_008499757.1 YggT family protein -
  MUY33_RS19820 (MUY33_19820) yggU 4087343..4087639 (+) 297 WP_008499758.1 DUF167 family protein YggU -
  MUY33_RS19825 (MUY33_19825) - 4087643..4088236 (+) 594 WP_032653325.1 XTP/dITP diphosphatase -
  MUY33_RS19830 (MUY33_19830) hemW 4088229..4089377 (+) 1149 WP_032653327.1 radical SAM family heme chaperone HemW -
  MUY33_RS19835 (MUY33_19835) - 4089434..4089796 (+) 363 WP_023344874.1 endonuclease domain-containing protein -
  MUY33_RS19840 (MUY33_19840) - 4089872..4090588 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  MUY33_RS19845 (MUY33_19845) - 4090645..4090971 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35479.36 Da        Isoelectric Point: 6.5865

>NTDB_id=675922 MUY33_RS19805 WP_032653323.1 4085059..4086039(-) (pilT) [Enterobacter roggenkampii strain K666]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPSPDLGALLKAWLNDEQQGIWWANGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRAIPELLTNASGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALHSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLVPDLQDGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSQAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=675922 MUY33_RS19805 WP_032653323.1 4085059..4086039(-) (pilT) [Enterobacter roggenkampii strain K666]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCTCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCCTCTCCGGACCTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAACGGGCAGGTGGATTTTGCCGCGACGGTGGCGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGAGTCTCTGTGACGCTGCGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCGCTGGG
CGCGCCGAGGGCAATCCCGGAACTGTTAACCAATGCGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATTCTCACGCTCGAAGATCCGGTGGAG
TTTATCTATCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATCGGCCAGCATAGCCCGTCATTTGCCGACGCGCTGCA
CAGTGCCTTACGCCAGGATCCGGACGTGATTTTGCTGGGAGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGTTAACGG
CAGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAAGCTGGTGCC
TGACTTACAGGACGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACCGCGGCGGCGGCGAATCTCATTCGCGAGGGAA
AAACGTGGCAGCTGCCCGGTATCATTCAAACCGGTCAGCAGGCAGGCATGCAGAACTTTGACCAGAGCCAGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Legionella pneumophila strain Lp02

47.516

98.773

0.469
  pilT Legionella pneumophila strain ERS1305867

47.516

98.773

0.469
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Neisseria meningitidis 8013

47.5

98.16

0.466
  pilT Neisseria gonorrhoeae MS11

47.5

98.16

0.466
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.708

97.853

0.457
  pilT Acinetobacter nosocomialis M2

46.708

97.853

0.457
  pilT Acinetobacter baumannii D1279779

46.708

97.853

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.082

97.546

0.42
  pilU Pseudomonas stutzeri DSM 10701

39.577

100

0.402
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362