Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MUY30_RS18590 Genome accession   NZ_CP095165
Coordinates   3903520..3904500 (-) Length   326 a.a.
NCBI ID   WP_063418227.1    Uniprot ID   -
Organism   Enterobacter roggenkampii strain K475-2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3898520..3909500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUY30_RS18560 (MUY30_18560) - 3899401..3899898 (+) 498 WP_039024096.1 SprT family zinc-dependent metalloprotease -
  MUY30_RS18565 (MUY30_18565) endA 3899993..3900700 (+) 708 WP_021242074.1 deoxyribonuclease I -
  MUY30_RS18570 (MUY30_18570) rsmE 3900752..3901483 (+) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MUY30_RS18575 (MUY30_18575) gshB 3901503..3902450 (+) 948 WP_008499751.1 glutathione synthase -
  MUY30_RS18580 (MUY30_18580) - 3902533..3903093 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  MUY30_RS18585 (MUY30_18585) ruvX 3903093..3903509 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  MUY30_RS18590 (MUY30_18590) pilT 3903520..3904500 (-) 981 WP_063418227.1 type IV pilus twitching motility protein PilT Machinery gene
  MUY30_RS18595 (MUY30_18595) - 3904518..3905219 (+) 702 WP_008499756.1 YggS family pyridoxal phosphate-dependent enzyme -
  MUY30_RS18600 (MUY30_18600) - 3905241..3905807 (+) 567 WP_008499757.1 YggT family protein -
  MUY30_RS18605 (MUY30_18605) yggU 3905804..3906100 (+) 297 WP_008499758.1 DUF167 family protein YggU -
  MUY30_RS18610 (MUY30_18610) - 3906104..3906697 (+) 594 WP_023294524.1 XTP/dITP diphosphatase -
  MUY30_RS18615 (MUY30_18615) hemW 3906690..3907838 (+) 1149 WP_008499760.1 radical SAM family heme chaperone HemW -
  MUY30_RS18620 (MUY30_18620) - 3908006..3908722 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  MUY30_RS18625 (MUY30_18625) - 3908779..3909105 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35453.45 Da        Isoelectric Point: 6.9103

>NTDB_id=675899 MUY30_RS18590 WP_063418227.1 3903520..3904500(-) (pilT) [Enterobacter roggenkampii strain K475-2]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPSPDLGALLKAWLNDEQQGIWWANGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRVIPELLTNASGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSARCLIQQREIGQHSPSFADALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLVPDLQDGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=675899 MUY30_RS18590 WP_063418227.1 3903520..3904500(-) (pilT) [Enterobacter roggenkampii strain K475-2]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCTCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTTCTCCGGACCTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAACGGGCAGGTGGATTTTGCCGCTACGGTGGCGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGAGTCTCTGTAACGCTACGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCGCTGGG
CGCACCGAGGGTTATCCCGGAACTGTTAACCAATGCGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATACTGACGGCCATATTCTCACGCTCGAAGATCCGGTGGAG
TTTATCTATCAGAGCGCACGTTGCCTGATCCAGCAGCGGGAGATCGGTCAGCACAGCCCGTCATTTGCCGACGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTGATTTTGCTGGGAGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAAGCTGGTTCC
TGACTTACAGGACGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACCGCGGCGGCGGCAAATCTGATACGAGAAGGAA
AAACGTGGCAGCTGCCCGGCATTATTCAAACCGGACAGCAGGCAGGCATGCAGAACTTTGACCAAAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.188

98.16

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.963

99.08

0.436
  pilU Pseudomonas stutzeri DSM 10701

40.181

100

0.408
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368