Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MU411_RS03745 Genome accession   NZ_CP095150
Coordinates   751782..752762 (+) Length   326 a.a.
NCBI ID   WP_023282988.1    Uniprot ID   A0A332YTV9
Organism   Klebsiella pneumoniae strain Iso00073     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 746782..757762
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU411_RS03715 - 747113..748159 (-) 1047 WP_004185615.1 sugar ABC transporter substrate-binding protein -
  MU411_RS03720 hemW 748452..749591 (-) 1140 WP_048269508.1 radical SAM family heme chaperone HemW -
  MU411_RS03725 - 749584..750177 (-) 594 WP_002916615.1 XTP/dITP diphosphatase -
  MU411_RS03730 yggU 750190..750480 (-) 291 WP_016947438.1 DUF167 family protein YggU -
  MU411_RS03735 - 750477..751043 (-) 567 WP_002916613.1 YggT family protein -
  MU411_RS03740 - 751064..751765 (-) 702 WP_004185610.1 YggS family pyridoxal phosphate-dependent enzyme -
  MU411_RS03745 pilT 751782..752762 (+) 981 WP_023282988.1 type IV pilus twitching motility protein PilT Machinery gene
  MU411_RS03750 - 753111..753764 (+) 654 WP_048269507.1 LuxR family transcriptional regulator -
  MU411_RS03755 ruvX 753817..754233 (-) 417 WP_025713492.1 Holliday junction resolvase RuvX -
  MU411_RS03760 - 754233..754796 (-) 564 WP_025713491.1 YqgE/AlgH family protein -
  MU411_RS03765 gshB 754969..755919 (-) 951 WP_025713490.1 glutathione synthase -
  MU411_RS03770 rsmE 755940..756671 (-) 732 WP_023282987.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MU411_RS03775 endA 756763..757470 (-) 708 WP_023282986.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35297.75 Da        Isoelectric Point: 6.8575

>NTDB_id=675738 MU411_RS03745 WP_023282988.1 751782..752762(+) (pilT) [Klebsiella pneumoniae strain Iso00073]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGRLEPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALNLACGARLRA
SAFAHTRGISLVLRLLPEQCPRLDTLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGAL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=675738 MU411_RS03745 WP_023282988.1 751782..752762(+) (pilT) [Klebsiella pneumoniae strain Iso00073]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAGGCTGGAGCCCGCGCCCTTTCCCGCGCCGGATATTGCGAATTTACTCAATGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGAACCTGGCCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGCCTCCTTCCTGAACAGTGCCCGCGTCTGGACACGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGGGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGACAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGCGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAACGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A332YTV9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

100

0.518
  pilT Vibrio cholerae strain A1552

51.368

100

0.518
  pilT Acinetobacter baylyi ADP1

48.936

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.024

100

0.485
  pilT Acinetobacter baumannii strain A118

48.024

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

47.72

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.148

99.387

0.479
  pilT Legionella pneumophila strain Lp02

48.148

99.387

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Vibrio cholerae strain A1552

41.049

99.387

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

40.187

98.466

0.396