Detailed information NTG0675714

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: MU412_RS03670
• Genome accession: NZ_CP095145
• Coordinates: 735913..736893 (+)
• Length: 326 a.a.
• NCBI ID: WP_096903188.1
• Uniprot ID: A0A486S515
• Organism: Klebsiella pneumoniae strain Iso00100
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	737004..737984	735913..736893	flank	111

Gene organization within MGE regions

Location: 735913..737984

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MU412_RS03670	pilT	735913..736893 (+)	981	WP_096903188.1	type IV pilus twitching motility protein PilT	Machinery gene
MU412_RS03675	-	737004..737984 (+)	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35457.93 Da
• Isoelectric Point: 6.6124

>NTDB_id=675714 MU412_RS03670 WP_096903188.1 735913..736893(+) (pilT) [Klebsiella pneumoniae strain Iso00100]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGRLEPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALNLACGARLRA
SAFAHTRGISLVLRLLPEQCPRLDMLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVEHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPEVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGAL

Nucleotide

• Length: 981 bp

>NTDB_id=675714 MU412_RS03670 WP_096903188.1 735913..736893(+) (pilT) [Klebsiella pneumoniae strain Iso00100]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAGGCTGGAGCCCGCGCCCTTTCCCGCGCCGGATATTGCGAATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGAACCTGGCCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGTCTCCTTCCTGAACAGTGCCCGCGTCTGGATATGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGAGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGACAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGAGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAACGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (11-266)

3D structure

• AlphaFold DB: A0A486S515

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	51.064	100	0.515
pilT	Vibrio cholerae strain A1552	51.064	100	0.515
pilT	Acinetobacter baylyi ADP1	48.632	100	0.491
pilT	Neisseria meningitidis 8013	47.866	100	0.482
pilT	Acinetobacter baumannii D1279779	47.72	100	0.482
pilT	Acinetobacter baumannii strain A118	47.72	100	0.482
pilT	Neisseria gonorrhoeae MS11	47.561	100	0.479
pilT	Acinetobacter nosocomialis M2	47.416	100	0.479
pilT	Legionella pneumophila strain ERS1305867	47.84	99.387	0.475
pilT	Legionella pneumophila strain Lp02	47.84	99.387	0.475
pilT	Pseudomonas stutzeri DSM 10701	47.095	100	0.472
pilT	Pseudomonas aeruginosa PAK	46.483	100	0.466
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.479	97.239	0.433
pilU	Vibrio cholerae strain A1552	40.741	99.387	0.405
pilU	Pseudomonas stutzeri DSM 10701	39.091	100	0.396
pilU	Acinetobacter baylyi ADP1	40.187	98.466	0.396