Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MU415_RS03755 Genome accession   NZ_CP095132
Coordinates   752953..753933 (+) Length   326 a.a.
NCBI ID   WP_020801960.1    Uniprot ID   A0A331PSC5
Organism   Klebsiella pneumoniae strain Iso00267     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 747953..758933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU415_RS03720 - 748489..748815 (+) 327 WP_002916620.1 YggL family protein -
  MU415_RS03725 - 748866..749585 (+) 720 WP_002916619.1 DUF2884 domain-containing protein -
  MU415_RS03730 hemW 749626..750762 (-) 1137 WP_004149812.1 radical SAM family heme chaperone HemW -
  MU415_RS03735 - 750755..751348 (-) 594 WP_002916615.1 XTP/dITP diphosphatase -
  MU415_RS03740 yggU 751361..751651 (-) 291 WP_002916614.1 DUF167 family protein YggU -
  MU415_RS03745 - 751648..752214 (-) 567 WP_002916613.1 YggT family protein -
  MU415_RS03750 - 752235..752936 (-) 702 WP_020803833.1 YggS family pyridoxal phosphate-dependent enzyme -
  MU415_RS03755 pilT 752953..753933 (+) 981 WP_020801960.1 type IV pilus twitching motility protein PilT Machinery gene
  MU415_RS03760 - 754282..754935 (+) 654 WP_020803826.1 LuxR family transcriptional regulator -
  MU415_RS03765 ruvX 754988..755404 (-) 417 WP_002916605.1 Holliday junction resolvase RuvX -
  MU415_RS03770 - 755404..755967 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  MU415_RS03775 gshB 756142..757092 (-) 951 WP_002916600.1 glutathione synthase -
  MU415_RS03780 rsmE 757113..757844 (-) 732 WP_002916597.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MU415_RS03785 endA 757936..758643 (-) 708 WP_002916589.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35284.75 Da        Isoelectric Point: 6.8575

>NTDB_id=675643 MU415_RS03755 WP_020801960.1 752953..753933(+) (pilT) [Klebsiella pneumoniae strain Iso00267]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGRLEPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALTLACGARLRA
SAFAHTRGISLVLRLLPEQCPRLDTLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGAL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=675643 MU415_RS03755 WP_020801960.1 752953..753933(+) (pilT) [Klebsiella pneumoniae strain Iso00267]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCACG
CTGGCGGCGGCAGGGCAGGCTGGAGCCCGCGCCCTTTCCCGCGCCGGATATTGCGAATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGCCTCCTTCCTGAACAGTGCCCGCGTCTGGACACGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGGGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGACAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGCGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAACGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A331PSC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

100

0.518
  pilT Vibrio cholerae strain A1552

51.368

100

0.518
  pilT Acinetobacter baylyi ADP1

48.936

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.024

100

0.485
  pilT Acinetobacter baumannii strain A118

48.024

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

47.72

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.148

99.387

0.479
  pilT Legionella pneumophila strain Lp02

48.148

99.387

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Vibrio cholerae strain A1552

41.049

99.387

0.408
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Acinetobacter baylyi ADP1

39.875

98.466

0.393