Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MUK77_RS04545 Genome accession   NZ_CP095086
Coordinates   926821..927801 (+) Length   326 a.a.
NCBI ID   WP_001683272.1    Uniprot ID   A0A0H3EPC5
Organism   Escherichia coli isolate EC5654     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 921821..932801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUK77_RS04520 (MUK77_04520) hemW 923498..924634 (-) 1137 WP_016238481.1 radical SAM family heme chaperone HemW -
  MUK77_RS04525 (MUK77_04525) rdgB 924627..925220 (-) 594 WP_001174738.1 XTP/dITP diphosphatase -
  MUK77_RS04530 (MUK77_04530) yggU 925228..925518 (-) 291 WP_001277214.1 DUF167 family protein YggU -
  MUK77_RS04535 (MUK77_04535) yggT 925515..926081 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  MUK77_RS04540 (MUK77_04540) yggS 926099..926803 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  MUK77_RS04545 (MUK77_04545) pilT 926821..927801 (+) 981 WP_001683272.1 type IV pilus twitching motility protein PilT Machinery gene
  MUK77_RS04550 (MUK77_04550) ruvX 927975..928391 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  MUK77_RS04555 (MUK77_04555) yqgE 928391..928954 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  MUK77_RS04560 (MUK77_04560) gshB 929063..930013 (-) 951 WP_000593260.1 glutathione synthase -
  MUK77_RS04565 (MUK77_04565) rsmE 930026..930757 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MUK77_RS04570 (MUK77_04570) endA 930837..931544 (-) 708 WP_001305312.1 deoxyribonuclease I -
  MUK77_RS04575 (MUK77_04575) yggI 931639..932136 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35972.15 Da        Isoelectric Point: 5.7980

>NTDB_id=675149 MUK77_RS04545 WP_001683272.1 926821..927801(+) (pilT) [Escherichia coli isolate EC5654]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=675149 MUK77_RS04545 WP_001683272.1 926821..927801(+) (pilT) [Escherichia coli isolate EC5654]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGAGAATGGAAGCTGCGCCGTTTGACGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGATTGCGCGGC
AGTGCGTTCGCACAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAGCAGCGGGAGATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACATTACATACGCGTGGTGCGGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCTGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGTTGTCACAAAAACTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGAGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3EPC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

45.26

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365