Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MT472_RS04270 Genome accession   NZ_CP094999
Coordinates   868215..869195 (+) Length   326 a.a.
NCBI ID   WP_042946042.1    Uniprot ID   -
Organism   Klebsiella michiganensis strain fxq     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 863215..874195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MT472_RS04240 (MT472_04240) - 863597..864583 (-) 987 WP_014839537.1 TRAP transporter substrate-binding protein -
  MT472_RS04245 (MT472_04245) hemW 864888..866024 (-) 1137 WP_014839536.1 radical SAM family heme chaperone HemW -
  MT472_RS04250 (MT472_04250) - 866017..866610 (-) 594 WP_004854157.1 XTP/dITP diphosphatase -
  MT472_RS04255 (MT472_04255) yggU 866623..866913 (-) 291 WP_004854155.1 DUF167 family protein YggU -
  MT472_RS04260 (MT472_04260) - 866910..867476 (-) 567 WP_004105714.1 YggT family protein -
  MT472_RS04265 (MT472_04265) - 867494..868198 (-) 705 WP_042946043.1 YggS family pyridoxal phosphate-dependent enzyme -
  MT472_RS04270 (MT472_04270) pilT 868215..869195 (+) 981 WP_042946042.1 type IV pilus twitching motility protein PilT Machinery gene
  MT472_RS04275 (MT472_04275) - 869544..870197 (+) 654 WP_014226837.1 LuxR C-terminal-related transcriptional regulator -
  MT472_RS04280 (MT472_04280) ruvX 870253..870669 (-) 417 WP_014226836.1 Holliday junction resolvase RuvX -
  MT472_RS04285 (MT472_04285) - 870669..871232 (-) 564 WP_014226835.1 YqgE/AlgH family protein -
  MT472_RS04290 (MT472_04290) gshB 871409..872356 (-) 948 WP_014226834.1 glutathione synthase -
  MT472_RS04295 (MT472_04295) rsmE 872377..873108 (-) 732 WP_032692850.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MT472_RS04300 (MT472_04300) endA 873200..873907 (-) 708 WP_014226832.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35205.34 Da        Isoelectric Point: 6.6548

>NTDB_id=674755 MT472_RS04270 WP_042946042.1 868215..869195(+) (pilT) [Klebsiella michiganensis strain fxq]
MELEEIVALSVKHNVSDLHLCNASTPRWRRQGKLEPAPFTSPDIGKILADWLNAEQLAQWQACGQVDFALTPAGGPRLRA
SAFVHNRGLSLALRLLAETCPQLADLGAPAALTELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLNGHILTLEDPVE
FIHHSERSLIQQREIGRHCPSFAAALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRSQLAGSLCAVLAQKLFPDTAGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQFGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=674755 MT472_RS04270 WP_042946042.1 868215..869195(+) (pilT) [Klebsiella michiganensis strain fxq]
ATGGAACTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCTTCAACGCCGCG
CTGGCGTCGCCAGGGCAAGCTGGAGCCTGCGCCTTTTACCTCTCCGGATATTGGCAAGATACTCGCTGATTGGCTCAACG
CCGAGCAGCTCGCCCAGTGGCAGGCCTGCGGGCAGGTTGATTTCGCGCTGACTCCGGCCGGCGGCCCGCGCCTGCGCGCC
AGCGCATTTGTCCACAACCGCGGCCTGTCGCTGGCCCTGCGTCTGCTGGCGGAAACCTGTCCGCAGCTGGCGGATCTCGG
CGCGCCGGCCGCCTTAACGGAGCTGCTCAATGAGGAGAGCGGGCTTATCCTGGTCACGGGCGCCACCGGTAGCGGGAAAT
CCACCACCCTGGCGGCGATGGTGGGCTATCTTAATCAGCGCCTCAATGGCCATATCCTGACGCTGGAAGATCCGGTTGAG
TTTATTCATCACAGCGAGCGCAGCCTGATCCAGCAGCGGGAAATCGGTCGTCACTGCCCCTCATTTGCCGCGGCCCTGCG
CGCTGCGCTGCGTCAGGACCCGGATGTCATTCTGCTAGGGGAACTGCGTGACAGCGAAACGATCCGCCTGGCATTGACCG
CGGCGGAGACCGGACATCTGGTTATGGCGACGCTGCATACGCGCGGCGCGGCACAGGCGGTTGAAAGGCTGGTGGATGTT
TTTCCTGCCCAGGAGAAAGAGCAGGTTCGTAGCCAGCTGGCGGGAAGTTTATGCGCGGTGCTGGCACAAAAATTGTTTCC
GGATACTGCTGGCGGAAGAGTTGCGCTATACGAGCTTTTGGTTAATACCCCGGCGGTGGCGAATTTGATCCGCGAGGGGA
AAACGCATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAATTCGGGATGCAAACTTTTACGCAAAGCTTTCAGCAACGT
ACGGCTGCGGGTCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.544

100

0.5
  pilT Vibrio cholerae strain A1552

49.544

100

0.5
  pilT Neisseria meningitidis 8013

48.485

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.182

100

0.488
  pilT Legionella pneumophila strain Lp02

47.799

97.546

0.466
  pilT Legionella pneumophila strain ERS1305867

47.799

97.546

0.466
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Acinetobacter baumannii strain A118

45.152

100

0.457
  pilT Acinetobacter baumannii D1279779

45.152

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.289

100

0.457
  pilT Acinetobacter nosocomialis M2

44.848

100

0.454
  pilT Acinetobacter baylyi ADP1

44.681

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

40.854

100

0.411
  pilU Vibrio cholerae strain A1552

40.373

98.773

0.399
  pilU Acinetobacter baylyi ADP1

38.08

99.08

0.377