Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TAO_RS00390 Genome accession   NZ_AP014836
Coordinates   71289..72326 (+) Length   345 a.a.
NCBI ID   WP_096526111.1    Uniprot ID   A0A1Q2SJZ7
Organism   Candidatus Nitrosoglobus terrae strain TAO100     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 66289..77326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TAO_RS00360 (TAO_0071) - 66616..67326 (-) 711 WP_096526105.1 tetratricopeptide repeat protein -
  TAO_RS00365 (TAO_0072) - 67510..68694 (+) 1185 WP_231910515.1 AmpG family muropeptide MFS transporter -
  TAO_RS00370 (TAO_0073) - 68699..69007 (-) 309 WP_096526107.1 DUF167 family protein -
  TAO_RS00375 (TAO_0074) - 69015..69584 (-) 570 WP_096526108.1 YggT family protein -
  TAO_RS00380 (TAO_0075) proC 69574..70431 (-) 858 WP_096526109.1 pyrroline-5-carboxylate reductase -
  TAO_RS00385 (TAO_0076) - 70459..71172 (-) 714 WP_096526110.1 YggS family pyridoxal phosphate-dependent enzyme -
  TAO_RS00390 (TAO_0077) pilT 71289..72326 (+) 1038 WP_096526111.1 type IV pilus twitching motility protein PilT Machinery gene
  TAO_RS00395 (TAO_0078) - 72642..74129 (+) 1488 WP_096526112.1 MBOAT family protein -
  TAO_RS00400 (TAO_0079) - 74129..75157 (+) 1029 WP_096526113.1 hypothetical protein -
  TAO_RS00405 (TAO_0080) - 75653..76294 (+) 642 WP_096526114.1 hypothetical protein -
  TAO_RS00410 (TAO_0081) - 76497..76862 (-) 366 WP_096526115.1 DUF6164 family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38294.02 Da        Isoelectric Point: 7.2806

>NTDB_id=67002 TAO_RS00390 WP_096526111.1 71289..72326(+) (pilT) [Candidatus Nitrosoglobus terrae strain TAO100]
MDITALLAFSVKNSASDLHLSAGLPPMLRVDGEIHRIDLPALDHKEIHSTIYSIMNDKQRKSFEEFLETDFSFEIPGLAR
FRTNVFTQNRGLAAVFRTIPSKVLTLEELNAPSGFKDIANNSRGIVLVTGPTGSGKSTTLAAMINYKNENDFAHILTIED
PIEFVHESKKSLINQREIHRDTYGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPATEKAMVRSMLSESLRAVISQTLLKKAGGGRVAAHEIMVGTPAIRNLIREDKIPQMYSAIQTGQSVGMQTLDQCL
LDLIRRNVVTKQEAVKKAVNKDIFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=67002 TAO_RS00390 WP_096526111.1 71289..72326(+) (pilT) [Candidatus Nitrosoglobus terrae strain TAO100]
ATGGATATCACTGCGTTACTGGCTTTTAGTGTGAAAAATTCTGCTTCCGACTTACACCTCTCTGCGGGGCTTCCGCCAAT
GCTTCGGGTAGATGGTGAGATACATCGGATCGACTTACCGGCTCTGGATCATAAAGAAATTCATAGCACTATCTACAGTA
TCATGAATGATAAGCAACGTAAGAGTTTCGAGGAATTCCTTGAAACTGATTTTTCCTTTGAAATCCCTGGATTGGCTCGT
TTTCGGACTAATGTATTTACCCAGAATCGCGGATTGGCCGCGGTGTTCCGCACTATTCCTTCCAAAGTATTAACTTTAGA
AGAACTTAATGCTCCCTCCGGATTTAAGGATATTGCTAATAATTCTCGGGGAATTGTATTAGTAACAGGGCCAACAGGTT
CTGGGAAATCCACTACCCTAGCGGCTATGATTAATTATAAAAATGAGAATGATTTTGCGCATATTTTGACTATTGAAGAT
CCTATTGAGTTTGTGCATGAGAGCAAAAAGAGCCTTATTAATCAAAGAGAAATACATCGAGATACTTATGGTTTTAATGA
AGCGTTACGTTCAGCCTTACGGGAAGATCCTGATATTATCTTAGTAGGTGAAATGCGAGATCTAGAAACCATTCGCTTAG
CTTTAACAGCGGCAGAGACAGGTCATCTAGTTTTTGGTACTTTACATACCAGTTCAGCAGCAAAGACGATTGATCGTATT
ATTGATGTTTTTCCAGCTACGGAGAAGGCTATGGTACGTTCTATGCTTTCTGAGTCCCTGCGAGCGGTTATCTCTCAAAC
TCTACTTAAAAAGGCAGGTGGGGGTCGAGTAGCTGCCCATGAGATTATGGTAGGTACGCCCGCCATTCGTAATCTTATTC
GTGAGGACAAGATCCCTCAAATGTACTCTGCTATTCAAACGGGGCAGAGTGTAGGTATGCAAACCCTAGATCAATGTTTG
TTGGATCTAATCCGCCGTAATGTCGTCACGAAACAGGAGGCGGTCAAAAAAGCGGTGAATAAGGATATTTTTCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Q2SJZ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

76.163

99.71

0.759
  pilT Acinetobacter nosocomialis M2

76.163

99.71

0.759
  pilT Acinetobacter baumannii D1279779

76.163

99.71

0.759
  pilT Pseudomonas aeruginosa PAK

76.923

97.971

0.754
  pilT Pseudomonas stutzeri DSM 10701

75.581

99.71

0.754
  pilT Acinetobacter baylyi ADP1

77.508

95.362

0.739
  pilT Legionella pneumophila strain Lp02

70.93

99.71

0.707
  pilT Legionella pneumophila strain ERS1305867

70.93

99.71

0.707
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.681

0.678
  pilT Vibrio cholerae strain A1552

69.436

97.681

0.678
  pilT Neisseria meningitidis 8013

67.347

99.42

0.67
  pilT Neisseria gonorrhoeae MS11

67.055

99.42

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.504
  pilU Acinetobacter baylyi ADP1

40.882

98.551

0.403
  pilU Vibrio cholerae strain A1552

38.728

100

0.388
  pilU Pseudomonas stutzeri DSM 10701

38.439

100

0.386

Multiple sequence alignment