Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MST28_RS04000 Genome accession   NZ_CP094479
Coordinates   800046..801026 (+) Length   326 a.a.
NCBI ID   WP_021468298.1    Uniprot ID   -
Organism   Klebsiella pneumoniae strain LXR-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 795046..806026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MST28_RS03970 (MST28_03970) - 795377..796423 (-) 1047 WP_021468300.1 sugar ABC transporter substrate-binding protein -
  MST28_RS03975 (MST28_03975) hemW 796716..797855 (-) 1140 WP_016529066.1 radical SAM family heme chaperone HemW -
  MST28_RS03980 (MST28_03980) - 797848..798441 (-) 594 WP_002916615.1 XTP/dITP diphosphatase -
  MST28_RS03985 (MST28_03985) yggU 798454..798744 (-) 291 WP_002916614.1 DUF167 family protein YggU -
  MST28_RS03990 (MST28_03990) - 798741..799307 (-) 567 WP_002916613.1 YggT family protein -
  MST28_RS03995 (MST28_03995) - 799328..800029 (-) 702 WP_004144780.1 YggS family pyridoxal phosphate-dependent enzyme -
  MST28_RS04000 (MST28_04000) pilT 800046..801026 (+) 981 WP_021468298.1 type IV pilus twitching motility protein PilT Machinery gene
  MST28_RS04005 (MST28_04005) - 801375..802028 (+) 654 WP_002916607.1 LuxR family transcriptional regulator -
  MST28_RS04010 (MST28_04010) ruvX 802081..802497 (-) 417 WP_002916605.1 Holliday junction resolvase RuvX -
  MST28_RS04015 (MST28_04015) - 802497..803060 (-) 564 WP_002916603.1 YqgE/AlgH family protein -
  MST28_RS04020 (MST28_04020) gshB 803235..804185 (-) 951 WP_243440294.1 glutathione synthase -
  MST28_RS04025 (MST28_04025) rsmE 804206..804937 (-) 732 WP_042936650.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MST28_RS04030 (MST28_04030) endA 805029..805736 (-) 708 WP_002916589.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35399.90 Da        Isoelectric Point: 6.7313

>NTDB_id=669824 MST28_RS04000 WP_021468298.1 800046..801026(+) (pilT) [Klebsiella pneumoniae strain LXR-1]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGRLEPAPFPAPDIANLLNDWLDAAQLLHWQEHGQIDFALNLACGARLRA
SAFAHTRGISLVLRLLPEQCPRLDMLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVEHLNQHLDGHILTLEDPVE
FIHHSERCLIQQREVGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGAL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=669824 MST28_RS04000 WP_021468298.1 800046..801026(+) (pilT) [Klebsiella pneumoniae strain LXR-1]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAGGCTGGAGCCCGCGCCCTTTCCCGCGCCGGATATTGCGAATTTACTCAACGACTGGCTCGATG
CCGCACAGTTACTGCACTGGCAGGAACACGGCCAGATTGACTTTGCGCTGAACCTGGCCTGCGGTGCGCGGCTGCGCGCC
AGCGCCTTCGCCCATACGCGTGGGATATCGCTGGTGCTGCGTCTCCTTCCTGAACAGTGCCCGCGTCTGGATATGCTCGG
CGCCCCGCCTGCGCTGAGCGAACTGCTGGCGGAAGAGAGTGGTTTGCTGTTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCAATGGTGGAGCACCTCAATCAGCATCTTGATGGCCATATTCTGACGCTTGAAGATCCGGTGGAA
TTTATTCATCACAGCGAGCGCTGTCTGATCCAGCAGCGGGAAGTTGGCCGTCACTGTCCTTCGTTCGCCGCGGCGCTCCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGGGAGCTACGCGACAGCGAAACCATTCGCCTGGCGTTAACCG
CGGCGGAGACCGGGCACCTGGTAATGGCGACATTACATACCCGCGGCGCGGCGCCGGCGGTGGAGAGATTGATCGATGTC
TTTCCGGCGGAGGAGAAAGATCAGGTTCGTAGCCAGCTTGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTCTGGTCAACACGCCAGCGGTGGCGAATTTGATCCGTGAAGGAA
AAGTACATCAATTGCCTGGTGTGATGCAGACCGGGATGCAGGCCGGAATGTTGACCTTTACCCAGAGTTTTCAGCAACGT
GTCGCTGCAGGCGCGCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.368

100

0.518
  pilT Vibrio cholerae strain A1552

51.368

100

0.518
  pilT Acinetobacter baylyi ADP1

48.936

100

0.494
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.024

100

0.485
  pilT Acinetobacter baumannii strain A118

48.024

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

47.72

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.148

99.387

0.479
  pilT Legionella pneumophila strain Lp02

48.148

99.387

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.795

97.239

0.436
  pilU Vibrio cholerae strain A1552

40.741

99.387

0.405
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Acinetobacter baylyi ADP1

40.187

98.466

0.396