Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PL145_RS18370 Genome accession   NZ_CP094338
Coordinates   4074068..4075081 (+) Length   337 a.a.
NCBI ID   WP_303313604.1    Uniprot ID   -
Organism   Dickeya fangzhongdai strain PL145     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4069068..4080081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PL145_RS18335 (PL145_18300) - 4069488..4069616 (-) 129 WP_038666077.1 entericidin A/B family lipoprotein -
  PL145_RS18340 (PL145_18305) hemW 4069748..4070887 (-) 1140 WP_303313601.1 radical SAM family heme chaperone HemW -
  PL145_RS18345 (PL145_18310) - 4070880..4071473 (-) 594 WP_042860940.1 XTP/dITP diphosphatase -
  PL145_RS18350 (PL145_18315) yggU 4071572..4071862 (-) 291 WP_038666082.1 DUF167 family protein YggU -
  PL145_RS18355 (PL145_18320) - 4071859..4072413 (-) 555 WP_038666084.1 YggT family protein -
  PL145_RS18360 (PL145_18325) proC 4072457..4073278 (-) 822 WP_303313602.1 pyrroline-5-carboxylate reductase -
  PL145_RS18365 (PL145_18330) - 4073312..4074046 (-) 735 WP_303313603.1 YggS family pyridoxal phosphate-dependent enzyme -
  PL145_RS18370 (PL145_18335) pilT 4074068..4075081 (+) 1014 WP_303313604.1 type IV pilus twitching motility protein PilT Machinery gene
  PL145_RS18375 (PL145_18340) - 4075225..4075416 (+) 192 WP_303313605.1 hypothetical protein -
  PL145_RS18380 (PL145_18345) - 4075395..4076015 (-) 621 WP_303313606.1 Fic family protein -
  PL145_RS18385 (PL145_18350) codA 4076185..4077435 (-) 1251 WP_303313607.1 cytosine deaminase -
  PL145_RS18390 (PL145_18355) - 4077438..4078325 (-) 888 WP_303313608.1 CdaR family transcriptional regulator -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 36774.00 Da        Isoelectric Point: 6.7154

>NTDB_id=669052 PL145_RS18370 WP_303313604.1 4074068..4075081(+) (pilT) [Dickeya fangzhongdai strain PL145]
MDVSEWVRSSVKQNVSDLHLCSGHPPVWRIDGQLVADVAQPPLHNDELEQWCGSWLEPACEQQLRQSGQTDCALTLPEGQ
RLRANLFMQRHGLSAALRLIPSHQPDLQTLGAPAVVAELADRPDGLILVTGATGSGKSTTLAAMIASLNQRRARHIITLE
DPIEFLHVSQRCLIQQREIGRHSDAFASALRAALREDPDVLLLGELRDGETIRLALTAAETGHLVLATLHTRRAVQAVDR
LVDAFPAEEKAFVRTQLAASLQAVVAQKLLPGAQGGRVALFEVLLATPAVSNLIREGKTHQLPSLLQTGGQVGMQTFEQS
YQQRCREGKLRPEMNAG

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=669052 PL145_RS18370 WP_303313604.1 4074068..4075081(+) (pilT) [Dickeya fangzhongdai strain PL145]
ATGGATGTGAGTGAATGGGTGCGGTCTAGTGTAAAACAGAATGTGTCCGATCTGCACCTGTGCAGCGGTCATCCGCCGGT
TTGGCGTATCGACGGGCAACTGGTGGCGGATGTTGCGCAGCCGCCGTTGCACAACGACGAGCTGGAGCAGTGGTGCGGCA
GCTGGCTGGAACCAGCCTGCGAGCAGCAACTGCGGCAAAGCGGACAGACCGACTGCGCGCTGACGCTGCCGGAGGGGCAG
CGGTTACGGGCCAATCTGTTTATGCAGCGCCACGGCCTGTCGGCGGCGCTGCGCCTGATCCCGTCACACCAACCTGACCT
GCAGACGCTGGGCGCGCCGGCAGTGGTAGCCGAGTTGGCCGATCGTCCCGATGGCCTGATTCTGGTGACCGGCGCAACCG
GCAGCGGTAAATCCACCACGCTGGCGGCGATGATCGCCTCGCTTAATCAACGCCGCGCGCGCCACATCATTACGCTGGAA
GACCCGATTGAGTTCCTGCATGTCAGCCAACGCTGTCTGATCCAACAGCGGGAGATTGGCCGTCATAGCGATGCGTTCGC
CAGCGCGCTGCGGGCGGCGCTGCGCGAGGACCCGGACGTGTTGCTGCTCGGCGAGTTGCGCGACGGCGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGCTGGCGACGCTGCATACCCGGCGGGCGGTGCAGGCGGTGGATCGG
CTGGTGGACGCATTCCCTGCCGAGGAAAAGGCGTTTGTCCGCACGCAACTGGCCGCCAGCCTGCAGGCGGTGGTGGCGCA
GAAACTGTTGCCGGGCGCGCAAGGCGGGCGCGTGGCGCTGTTTGAAGTGCTGCTCGCCACCCCGGCGGTCAGCAACCTGA
TCCGCGAAGGCAAAACCCACCAGTTGCCGAGCCTGCTGCAAACCGGCGGTCAGGTCGGCATGCAGACCTTTGAACAGAGT
TACCAGCAGCGATGCCGGGAGGGGAAGCTGCGGCCTGAAATGAATGCCGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

97.329

0.487
  pilT Vibrio cholerae strain A1552

50

97.329

0.487
  pilT Neisseria meningitidis 8013

48.657

99.407

0.484
  pilT Neisseria gonorrhoeae MS11

48.503

99.11

0.481
  pilT Pseudomonas aeruginosa PAK

48.348

98.813

0.478
  pilT Acinetobacter baylyi ADP1

48.78

97.329

0.475
  pilT Pseudomonas stutzeri DSM 10701

50

94.362

0.472
  pilT Acinetobacter baumannii D1279779

47.447

98.813

0.469
  pilT Acinetobacter nosocomialis M2

47.447

98.813

0.469
  pilT Acinetobacter baumannii strain A118

47.447

98.813

0.469
  pilT Legionella pneumophila strain Lp02

48.903

94.659

0.463
  pilT Legionella pneumophila strain ERS1305867

48.903

94.659

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.141

94.659

0.427
  pilU Pseudomonas stutzeri DSM 10701

38.323

99.11

0.38
  pilU Vibrio cholerae strain A1552

38.369

98.22

0.377
  pilU Acinetobacter baylyi ADP1

38.199

95.549

0.365

Multiple sequence alignment