Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MQE09_RS03975 Genome accession   NZ_CP094280
Coordinates   802254..803234 (+) Length   326 a.a.
NCBI ID   WP_045379585.1    Uniprot ID   -
Organism   Klebsiella aerogenes strain NY1464     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 797254..808234
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MQE09_RS03945 (MQE09_03945) - 797584..798630 (-) 1047 WP_224108516.1 sugar ABC transporter substrate-binding protein -
  MQE09_RS03950 (MQE09_03950) hemW 798924..800063 (-) 1140 WP_224108515.1 radical SAM family heme chaperone HemW -
  MQE09_RS03955 (MQE09_03955) - 800056..800649 (-) 594 WP_045379593.1 XTP/dITP diphosphatase -
  MQE09_RS03960 (MQE09_03960) yggU 800662..800952 (-) 291 WP_045379591.1 DUF167 family protein YggU -
  MQE09_RS03965 (MQE09_03965) - 800949..801515 (-) 567 WP_015369729.1 YggT family protein -
  MQE09_RS03970 (MQE09_03970) - 801533..802237 (-) 705 WP_045379588.1 YggS family pyridoxal phosphate-dependent enzyme -
  MQE09_RS03975 (MQE09_03975) pilT 802254..803234 (+) 981 WP_045379585.1 type IV pilus twitching motility protein PilT Machinery gene
  MQE09_RS03980 (MQE09_03980) - 803585..804238 (+) 654 WP_045379582.1 LuxR C-terminal-related transcriptional regulator -
  MQE09_RS03985 (MQE09_03985) ruvX 804291..804707 (-) 417 WP_015369733.1 Holliday junction resolvase RuvX -
  MQE09_RS03990 (MQE09_03990) - 804707..805270 (-) 564 WP_015369734.1 YqgE/AlgH family protein -
  MQE09_RS03995 (MQE09_03995) gshB 805404..806354 (-) 951 WP_045379580.1 glutathione synthase -
  MQE09_RS04000 (MQE09_04000) rsmE 806403..807134 (-) 732 WP_045379577.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MQE09_RS04005 (MQE09_04005) endA 807223..807930 (-) 708 WP_045379574.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35332.45 Da        Isoelectric Point: 6.7175

>NTDB_id=668553 MQE09_RS03975 WP_045379585.1 802254..803234(+) (pilT) [Klebsiella aerogenes strain NY1464]
MELEEIVALSVKHNVSDLHLCSAYPPRWRRLGKLEPAPFTSPDIEEILTRRLNDLQLAQWRADGQVDFALALADGPRLRA
SAFAHARGLSLALRLLPQRCPQLGDLGAPAALSELLNEESGLILVTGATGSGKSTTLAAMVDYLNQHRHGHILTLEDPVE
FIHASRNCLIQQREIGRHCSSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRSAAQAVERLVDV
FPAEEKEPVRNQLAGSLCAVAAQKLLPNASGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQAGMQTFAQSFQQR
ASAGEL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=668553 MQE09_RS03975 WP_045379585.1 802254..803234(+) (pilT) [Klebsiella aerogenes strain NY1464]
ATGGAGCTGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGCGCGTATCCGCCGCG
CTGGCGTCGTTTGGGAAAGCTTGAGCCTGCGCCGTTTACTTCGCCGGATATTGAGGAAATCCTCACTCGCAGGCTTAACG
ACCTACAGCTGGCGCAGTGGCGAGCCGATGGCCAGGTGGACTTTGCCCTCGCTCTGGCGGACGGGCCGCGGCTGCGCGCC
AGCGCTTTTGCCCACGCGCGAGGCTTGTCGTTGGCGCTGCGTCTGTTGCCGCAGCGATGCCCGCAGTTGGGTGATTTGGG
CGCGCCCGCCGCGCTTAGCGAACTATTAAATGAAGAGAGTGGCTTGATCCTGGTTACCGGCGCTACCGGTAGCGGCAAGT
CGACCACCCTGGCGGCGATGGTGGACTACCTCAATCAACATCGCCATGGTCATATTTTAACCCTTGAGGATCCCGTGGAG
TTTATTCACGCCAGCAGGAACTGCCTGATCCAGCAGCGCGAAATCGGCCGCCATTGCTCCTCCTTCGCGGCGGCGCTACG
CGTAGCGCTGCGTCAGGACCCGGACGTCATCTTATTAGGCGAGCTACGCGATAGCGAAACCATCCGCCTGGCGCTTACCG
CGGCAGAAACCGGGCACCTGGTAATGGCGACGTTGCATACCCGCAGCGCGGCGCAAGCCGTCGAGCGGCTGGTGGATGTG
TTCCCGGCGGAAGAAAAAGAGCCGGTTCGCAACCAGCTGGCTGGGAGTTTATGCGCCGTGGCGGCGCAAAAATTGTTGCC
AAATGCTTCCGGGGGCCGGGTTGCGCTATACGAACTTTTAGTGAACACGCCAGCGGTGGCAAATTTGATCCGCGAGGGCA
AAACGCATCAGCTTCCCGGCGTGATGCAAACCGGTCAACAGGCAGGAATGCAAACCTTTGCGCAGAGTTTTCAACAACGT
GCGTCAGCAGGTGAGTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(55-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.988

100

0.521
  pilT Vibrio cholerae strain A1552

51.988

100

0.521
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Legionella pneumophila strain ERS1305867

49.686

97.546

0.485
  pilT Legionella pneumophila strain Lp02

49.686

97.546

0.485
  pilT Acinetobacter nosocomialis M2

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.256

100

0.475
  pilT Acinetobacter baumannii D1279779

47.256

100

0.475
  pilT Pseudomonas stutzeri DSM 10701

48.418

96.933

0.469
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.741

97.239

0.445
  pilU Pseudomonas stutzeri DSM 10701

41.212

100

0.417
  pilU Vibrio cholerae strain A1552

41.615

98.773

0.411
  pilU Acinetobacter baylyi ADP1

38.462

99.693

0.383

Multiple sequence alignment