Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MON37_RS00065 Genome accession   NZ_CP094242
Coordinates   12951..13997 (-) Length   348 a.a.
NCBI ID   WP_003759783.1    Uniprot ID   A0A0C1GQQ0
Organism   Morococcus cerebrosus strain CIP 81.93     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7951..18997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MON37_RS00040 (MON37_00040) arsC 8535..8894 (+) 360 WP_039406990.1 arsenate reductase (glutaredoxin) -
  MON37_RS00045 (MON37_00045) - 8917..9588 (+) 672 WP_039406988.1 YdcF family protein -
  MON37_RS00050 (MON37_00050) - 9682..10218 (+) 537 WP_039406984.1 C40 family peptidase -
  MON37_RS00055 (MON37_00055) - 10601..11137 (-) 537 WP_003767081.1 porin family protein -
  MON37_RS00060 (MON37_00060) - 11549..12781 (-) 1233 WP_039406982.1 PilT/PilU family type 4a pilus ATPase -
  MON37_RS00065 (MON37_00065) pilT 12951..13997 (-) 1047 WP_003759783.1 type IV pilus twitching motility protein PilT Machinery gene
  MON37_RS00070 (MON37_00070) - 14177..14884 (+) 708 WP_039406979.1 YggS family pyridoxal phosphate-dependent enzyme -
  MON37_RS00075 (MON37_00075) - 14881..15276 (+) 396 WP_003779405.1 hypothetical protein -
  MON37_RS00080 (MON37_00080) proC 15367..16164 (+) 798 WP_039406975.1 pyrroline-5-carboxylate reductase -
  MON37_RS00085 (MON37_00085) - 16167..16721 (+) 555 WP_003759775.1 YggT family protein -
  MON37_RS00090 (MON37_00090) dksA 16915..17331 (+) 417 WP_003767066.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38114.58 Da        Isoelectric Point: 7.3729

>NTDB_id=668327 MON37_RS00065 WP_003759783.1 12951..13997(-) (pilT) [Morococcus cerebrosus strain CIP 81.93]
MQITDLLAFGVKNKASDLHLSAGMSPMIRVHGDIRRINLPEMSAEEVGNMVTSVMNDHQRKLFQQDLEVDFSFELPNVAR
FRVNAFTSNRGPAAVFRTIPSAVLTLEDLRAPRIFQKIADNPRGLVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKALVNQRELHQHTHSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLRAVISQTLLKTRDGNGRVAAHEILISTPAVRNLIRENKIAQINSALQTGQSHGMQTLDQA
LQTLVRQGTISPDVARSKAQNSDSMTIV

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=668327 MON37_RS00065 WP_003759783.1 12951..13997(-) (pilT) [Morococcus cerebrosus strain CIP 81.93]
ATGCAGATTACCGACTTACTCGCTTTCGGCGTTAAAAACAAAGCTTCCGACCTTCACTTAAGCGCCGGAATGTCCCCCAT
GATCCGCGTTCACGGCGACATCCGCCGCATCAACCTGCCCGAAATGTCCGCCGAAGAAGTCGGCAACATGGTTACTTCGG
TCATGAACGACCACCAGCGCAAACTGTTCCAACAAGATTTGGAAGTGGACTTCTCCTTCGAGCTGCCCAACGTCGCCCGC
TTCCGTGTCAACGCATTTACCTCCAACCGCGGCCCTGCCGCCGTCTTCCGTACCATTCCGAGCGCCGTATTGACGCTGGA
AGACCTGCGCGCCCCGCGTATTTTCCAAAAAATCGCCGACAACCCGCGCGGCTTGGTATTGGTAACCGGCCCGACCGGTT
CCGGTAAATCCACCACGCTGGCGGCGATGATCAACTACATCAACGAAACCCAACCCGCACACATCCTCACCATCGAAGAC
CCGATCGAGTTCGTCCACCAAAGTAAAAAAGCCCTCGTCAACCAGCGCGAGCTGCACCAACACACCCACAGCTTCGCCAA
CGCCCTGCGTTCCGCATTGCGTGAAGACCCCGACGTGATCCTCGTGGGTGAGATGCGCGACCCCGAAACCATCGGCCTCG
CGCTGACTGCCGCCGAAACCGGCCACTTGGTATTCGGCACGCTGCACACCACTGGCGCCGCCAAAACCGTGGACCGTATC
GTCGACGTATTCCCTGCCGGCGAAAAAGAGATGGTGCGTTCGATGCTGTCCGAATCCCTGCGCGCGGTTATTTCGCAAAC
CCTGCTGAAAACCCGCGACGGCAACGGCCGTGTCGCTGCACACGAAATCCTCATTTCCACCCCCGCAGTGCGCAACCTGA
TCCGCGAAAACAAAATCGCCCAAATCAACTCCGCGCTGCAAACCGGTCAATCACACGGCATGCAAACCCTCGACCAGGCG
CTGCAAACCCTCGTCCGCCAAGGTACCATCAGCCCCGACGTAGCCCGCAGCAAAGCGCAAAACAGCGACAGCATGACCAT
CGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C1GQQ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

87.896

99.713

0.876
  pilT Neisseria gonorrhoeae MS11

87.32

99.713

0.871
  pilT Acinetobacter nosocomialis M2

71.014

99.138

0.704
  pilT Acinetobacter baumannii D1279779

71.014

99.138

0.704
  pilT Acinetobacter baumannii strain A118

71.014

99.138

0.704
  pilT Pseudomonas stutzeri DSM 10701

70.145

99.138

0.695
  pilT Pseudomonas aeruginosa PAK

70.294

97.701

0.687
  pilT Acinetobacter baylyi ADP1

68.406

99.138

0.678
  pilT Legionella pneumophila strain Lp02

66.569

97.989

0.652
  pilT Legionella pneumophila strain ERS1305867

66.569

97.989

0.652
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.706

97.701

0.632
  pilT Vibrio cholerae strain A1552

64.706

97.701

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.622

97.414

0.503
  pilU Pseudomonas stutzeri DSM 10701

41.954

100

0.42
  pilU Vibrio cholerae strain A1552

39.368

100

0.394
  pilU Acinetobacter baylyi ADP1

40.361

95.402

0.385

Multiple sequence alignment